Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3589 and GFF3590 are separated by 28 nucleotides GFF3590 and GFF3591 are separated by 0 nucleotides GFF3591 and GFF3592 are separated by 82 nucleotides
GFF3589 - Transcriptional regulator, LysR family, at 100,250 to 101,164
GFF3589
GFF3590 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 101,193 to 102,107
GFF3590
GFF3591 - Glutathione reductase (EC 1.8.1.7), at 102,108 to 103,466
GFF3591
GFF3592 - hypothetical protein, at 103,549 to 104,856
GFF3592
Position (kb) 102
103
104 Strain fitness (log2 ratio) -2
-1
0
1
2 at 101.232 kb on + strand at 101.232 kb on + strand at 101.232 kb on + strand at 101.232 kb on + strand at 101.232 kb on + strand at 101.233 kb on - strand at 101.233 kb on - strand at 101.235 kb on + strand at 101.235 kb on + strand at 101.568 kb on + strand, within GFF3590 at 101.568 kb on + strand, within GFF3590 at 101.568 kb on + strand, within GFF3590 at 101.568 kb on + strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.751 kb on + strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.753 kb on + strand, within GFF3590 at 101.934 kb on + strand, within GFF3590 at 101.934 kb on + strand, within GFF3590 at 101.935 kb on - strand, within GFF3590 at 102.409 kb on + strand, within GFF3591 at 102.409 kb on + strand, within GFF3591 at 102.463 kb on + strand, within GFF3591 at 102.571 kb on + strand, within GFF3591 at 102.571 kb on + strand, within GFF3591 at 102.572 kb on - strand, within GFF3591 at 102.572 kb on - strand, within GFF3591 at 102.935 kb on - strand, within GFF3591 at 103.168 kb on + strand, within GFF3591 at 103.168 kb on + strand, within GFF3591 at 103.168 kb on + strand, within GFF3591 at 103.168 kb on + strand, within GFF3591 at 103.168 kb on + strand, within GFF3591 at 103.169 kb on + strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.169 kb on - strand, within GFF3591 at 103.258 kb on + strand, within GFF3591 at 103.259 kb on - strand, within GFF3591 at 103.300 kb on + strand, within GFF3591 at 103.300 kb on + strand, within GFF3591 at 103.300 kb on + strand, within GFF3591 at 103.300 kb on + strand, within GFF3591 at 103.300 kb on + strand, within GFF3591 at 103.300 kb on + strand, within GFF3591 at 103.300 kb on + strand, within GFF3591 at 103.301 kb on - strand, within GFF3591 at 103.301 kb on - strand, within GFF3591 at 103.306 kb on + strand, within GFF3591 at 103.306 kb on + strand, within GFF3591 at 103.306 kb on + strand, within GFF3591 at 103.307 kb on - strand, within GFF3591 at 103.320 kb on + strand, within GFF3591 at 103.321 kb on - strand, within GFF3591 at 103.321 kb on - strand, within GFF3591 at 104.120 kb on + strand, within GFF3592 at 104.120 kb on + strand, within GFF3592 at 104.120 kb on + strand, within GFF3592 at 104.184 kb on - strand, within GFF3592 at 104.345 kb on + strand, within GFF3592 at 104.346 kb on - strand, within GFF3592 at 104.346 kb on - strand, within GFF3592 at 104.346 kb on - strand, within GFF3592 at 104.346 kb on - strand, within GFF3592
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 101,232 + -0.4 101,232 + +0.7 101,232 + +0.6 101,232 + -0.1 101,232 + +0.1 101,233 - -0.5 101,233 - +0.3 101,235 + +0.5 101,235 + -1.1 101,568 + GFF3590 0.41 -1.0 101,568 + GFF3590 0.41 +1.2 101,568 + GFF3590 0.41 -0.5 101,568 + GFF3590 0.41 -0.2 101,569 - GFF3590 0.41 -0.9 101,569 - GFF3590 0.41 +0.2 101,569 - GFF3590 0.41 -1.3 101,569 - GFF3590 0.41 +0.1 101,751 + GFF3590 0.61 -0.9 101,752 - GFF3590 0.61 -0.1 101,752 - GFF3590 0.61 -2.0 101,752 - GFF3590 0.61 +0.7 101,752 - GFF3590 0.61 -0.1 101,752 - GFF3590 0.61 -0.1 101,752 - GFF3590 0.61 +0.5 101,753 + GFF3590 0.61 -1.4 101,934 + GFF3590 0.81 -0.1 101,934 + GFF3590 0.81 -1.0 101,935 - GFF3590 0.81 -0.3 102,409 + GFF3591 0.22 -0.1 102,409 + GFF3591 0.22 +1.2 102,463 + GFF3591 0.26 -1.1 102,571 + GFF3591 0.34 +0.0 102,571 + GFF3591 0.34 +0.2 102,572 - GFF3591 0.34 +0.3 102,572 - GFF3591 0.34 -1.3 102,935 - GFF3591 0.61 -0.5 103,168 + GFF3591 0.78 -0.1 103,168 + GFF3591 0.78 +0.6 103,168 + GFF3591 0.78 -1.1 103,168 + GFF3591 0.78 -0.7 103,168 + GFF3591 0.78 +0.3 103,169 + GFF3591 0.78 -0.5 103,169 - GFF3591 0.78 +0.4 103,169 - GFF3591 0.78 -0.1 103,169 - GFF3591 0.78 -1.4 103,169 - GFF3591 0.78 +0.6 103,169 - GFF3591 0.78 -0.3 103,169 - GFF3591 0.78 -1.1 103,169 - GFF3591 0.78 -0.6 103,169 - GFF3591 0.78 -1.7 103,258 + GFF3591 0.85 +0.4 103,259 - GFF3591 0.85 +0.9 103,300 + GFF3591 0.88 +1.1 103,300 + GFF3591 0.88 -0.8 103,300 + GFF3591 0.88 +1.5 103,300 + GFF3591 0.88 +0.5 103,300 + GFF3591 0.88 +0.3 103,300 + GFF3591 0.88 +2.3 103,300 + GFF3591 0.88 +0.6 103,301 - GFF3591 0.88 +0.9 103,301 - GFF3591 0.88 +0.3 103,306 + GFF3591 0.88 +0.9 103,306 + GFF3591 0.88 -1.3 103,306 + GFF3591 0.88 -0.5 103,307 - GFF3591 0.88 +0.2 103,320 + GFF3591 0.89 +0.6 103,321 - GFF3591 0.89 +0.9 103,321 - GFF3591 0.89 -0.9 104,120 + GFF3592 0.44 +0.5 104,120 + GFF3592 0.44 -0.7 104,120 + GFF3592 0.44 -0.4 104,184 - GFF3592 0.49 -0.8 104,345 + GFF3592 0.61 +0.4 104,346 - GFF3592 0.61 -0.9 104,346 - GFF3592 0.61 +0.2 104,346 - GFF3592 0.61 +0.4 104,346 - GFF3592 0.61 +0.5
Or see this region's nucleotide sequence