Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3479 and GFF3480 are separated by 105 nucleotides GFF3480 and GFF3481 overlap by 8 nucleotides GFF3481 and GFF3482 overlap by 4 nucleotides GFF3482 and GFF3483 overlap by 4 nucleotides
GFF3479 - no description, at 301,863 to 303,020
GFF3479
GFF3480 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 303,126 to 303,830
GFF3480
GFF3481 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 303,823 to 304,593
GFF3481
GFF3482 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 304,590 to 305,552
GFF3482
GFF3483 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 305,549 to 306,409
GFF3483
Position (kb) 303
304
305 Strain fitness (log2 ratio) -2
-1
0
1
2 at 302.943 kb on + strand at 302.943 kb on + strand at 302.943 kb on + strand at 302.943 kb on + strand at 302.944 kb on - strand at 302.944 kb on - strand at 302.944 kb on - strand at 303.018 kb on + strand at 303.018 kb on + strand at 303.018 kb on + strand at 303.018 kb on + strand at 303.018 kb on + strand at 303.018 kb on + strand at 303.019 kb on - strand at 303.019 kb on - strand at 303.019 kb on - strand at 303.019 kb on - strand at 303.019 kb on - strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.157 kb on - strand at 303.157 kb on - strand at 303.157 kb on - strand at 303.157 kb on - strand at 303.448 kb on + strand, within GFF3480 at 303.448 kb on + strand, within GFF3480 at 303.449 kb on - strand, within GFF3480 at 303.449 kb on - strand, within GFF3480 at 303.449 kb on - strand, within GFF3480 at 303.487 kb on + strand, within GFF3480 at 303.488 kb on - strand, within GFF3480 at 303.851 kb on + strand at 303.851 kb on + strand at 303.851 kb on + strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 304.177 kb on + strand, within GFF3481 at 304.177 kb on + strand, within GFF3481 at 304.177 kb on + strand, within GFF3481 at 304.178 kb on - strand, within GFF3481 at 304.178 kb on - strand, within GFF3481 at 304.692 kb on - strand, within GFF3482 at 304.697 kb on - strand, within GFF3482 at 304.697 kb on - strand, within GFF3482 at 304.738 kb on + strand, within GFF3482 at 304.738 kb on + strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.906 kb on + strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 305.053 kb on + strand, within GFF3482 at 305.240 kb on - strand, within GFF3482 at 305.240 kb on - strand, within GFF3482 at 305.240 kb on - strand, within GFF3482 at 305.251 kb on + strand, within GFF3482 at 305.252 kb on - strand, within GFF3482 at 305.314 kb on + strand, within GFF3482 at 305.314 kb on + strand, within GFF3482 at 305.314 kb on + strand, within GFF3482 at 305.315 kb on - strand, within GFF3482 at 305.315 kb on - strand, within GFF3482 at 305.339 kb on - strand, within GFF3482 at 305.389 kb on + strand, within GFF3482
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 302,943 + -0.2 302,943 + +1.2 302,943 + -0.9 302,943 + -0.6 302,944 - +0.3 302,944 - +1.0 302,944 - +0.3 303,018 + -0.9 303,018 + +0.6 303,018 + -2.5 303,018 + -0.2 303,018 + -0.5 303,018 + -0.2 303,019 - -1.0 303,019 - +0.5 303,019 - -0.3 303,019 - -0.6 303,019 - -0.1 303,028 - -0.3 303,028 - -0.2 303,157 - +0.6 303,157 - -0.9 303,157 - +1.4 303,157 - +1.7 303,448 + GFF3480 0.46 -0.1 303,448 + GFF3480 0.46 -0.2 303,449 - GFF3480 0.46 +0.3 303,449 - GFF3480 0.46 -0.7 303,449 - GFF3480 0.46 -0.7 303,487 + GFF3480 0.51 +0.3 303,488 - GFF3480 0.51 +0.2 303,851 + +0.2 303,851 + -0.2 303,851 + -1.3 303,852 - -1.1 303,852 - -0.6 303,852 - -0.5 303,852 - -1.4 303,852 - -0.5 303,852 - -1.2 304,177 + GFF3481 0.46 -0.6 304,177 + GFF3481 0.46 -2.5 304,177 + GFF3481 0.46 -0.9 304,178 - GFF3481 0.46 -0.3 304,178 - GFF3481 0.46 -2.5 304,692 - GFF3482 0.11 -0.4 304,697 - GFF3482 0.11 -0.1 304,697 - GFF3482 0.11 +0.2 304,738 + GFF3482 0.15 +1.9 304,738 + GFF3482 0.15 +1.1 304,739 - GFF3482 0.15 -1.0 304,739 - GFF3482 0.15 +1.1 304,739 - GFF3482 0.15 +1.5 304,739 - GFF3482 0.15 +1.3 304,739 - GFF3482 0.15 +1.9 304,739 - GFF3482 0.15 -1.5 304,906 + GFF3482 0.33 +0.7 304,907 - GFF3482 0.33 -0.2 304,907 - GFF3482 0.33 -0.7 304,907 - GFF3482 0.33 -1.5 304,907 - GFF3482 0.33 +0.5 305,053 + GFF3482 0.48 -0.4 305,240 - GFF3482 0.67 -0.4 305,240 - GFF3482 0.67 -1.2 305,240 - GFF3482 0.67 -0.7 305,251 + GFF3482 0.69 +1.1 305,252 - GFF3482 0.69 +1.7 305,314 + GFF3482 0.75 +0.3 305,314 + GFF3482 0.75 -0.7 305,314 + GFF3482 0.75 -0.7 305,315 - GFF3482 0.75 -0.7 305,315 - GFF3482 0.75 +1.9 305,339 - GFF3482 0.78 +0.6 305,389 + GFF3482 0.83 -0.7
Or see this region's nucleotide sequence