Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3447 and GFF3448 overlap by 4 nucleotides GFF3448 and GFF3449 are separated by 19 nucleotides GFF3449 and GFF3450 are separated by 130 nucleotides
GFF3447 - FIG00555764: hypothetical protein, at 269,842 to 270,732
GFF3447
GFF3448 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 270,729 to 271,493
GFF3448
GFF3449 - Intradiol ring-cleavage dioxygenase:Catechol dioxygenase, N-terminal, at 271,513 to 272,379
GFF3449
GFF3450 - Transcriptional regulator, LacI family, at 272,510 to 273,544
GFF3450
Position (kb) 271
272
273 Strain fitness (log2 ratio) -1
0
1 at 270.537 kb on - strand, within GFF3447 at 271.003 kb on + strand, within GFF3448 at 271.003 kb on + strand, within GFF3448 at 271.003 kb on + strand, within GFF3448 at 271.003 kb on + strand, within GFF3448 at 271.004 kb on - strand, within GFF3448 at 271.004 kb on - strand, within GFF3448 at 271.004 kb on - strand, within GFF3448 at 271.420 kb on + strand at 271.493 kb on + strand at 271.493 kb on + strand at 271.493 kb on + strand at 271.493 kb on + strand at 271.493 kb on + strand at 271.493 kb on + strand at 271.494 kb on - strand at 271.494 kb on - strand at 271.494 kb on - strand at 271.494 kb on - strand at 271.494 kb on - strand at 271.500 kb on - strand at 271.930 kb on + strand, within GFF3449 at 271.930 kb on + strand, within GFF3449 at 271.930 kb on + strand, within GFF3449 at 271.931 kb on - strand, within GFF3449 at 271.931 kb on - strand, within GFF3449 at 271.931 kb on - strand, within GFF3449 at 271.931 kb on - strand, within GFF3449 at 271.931 kb on - strand, within GFF3449 at 272.410 kb on + strand at 272.410 kb on + strand at 272.416 kb on + strand at 272.416 kb on + strand at 272.682 kb on - strand, within GFF3450 at 272.816 kb on + strand, within GFF3450 at 273.197 kb on + strand, within GFF3450 at 273.197 kb on + strand, within GFF3450 at 273.197 kb on + strand, within GFF3450 at 273.198 kb on - strand, within GFF3450 at 273.299 kb on + strand, within GFF3450
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 270,537 - GFF3447 0.78 +1.6 271,003 + GFF3448 0.36 -1.4 271,003 + GFF3448 0.36 -1.7 271,003 + GFF3448 0.36 -1.4 271,003 + GFF3448 0.36 -0.8 271,004 - GFF3448 0.36 +0.2 271,004 - GFF3448 0.36 -0.6 271,004 - GFF3448 0.36 -1.3 271,420 + -0.2 271,493 + -0.5 271,493 + -0.9 271,493 + +0.1 271,493 + +0.6 271,493 + -0.3 271,493 + +0.2 271,494 - -0.3 271,494 - +0.3 271,494 - -0.2 271,494 - -0.1 271,494 - -1.0 271,500 - -0.8 271,930 + GFF3449 0.48 -0.2 271,930 + GFF3449 0.48 -0.7 271,930 + GFF3449 0.48 -1.5 271,931 - GFF3449 0.48 -0.7 271,931 - GFF3449 0.48 -1.3 271,931 - GFF3449 0.48 -1.1 271,931 - GFF3449 0.48 -0.7 271,931 - GFF3449 0.48 +0.7 272,410 + -0.6 272,410 + -0.8 272,416 + -0.8 272,416 + +1.2 272,682 - GFF3450 0.17 -1.0 272,816 + GFF3450 0.30 +0.3 273,197 + GFF3450 0.66 -1.1 273,197 + GFF3450 0.66 -1.0 273,197 + GFF3450 0.66 +1.3 273,198 - GFF3450 0.66 -0.9 273,299 + GFF3450 0.76 -0.1
Or see this region's nucleotide sequence