Strain Fitness in Variovorax sp. SCN45 around GFF3362

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3360 and GFF3361 are separated by 22 nucleotidesGFF3361 and GFF3362 are separated by 9 nucleotidesGFF3362 and GFF3363 are separated by 151 nucleotides GFF3360 - no description, at 180,855 to 180,968 GFF3360 GFF3361 - Hydrolase, at 180,991 to 181,917 GFF3361 GFF3362 - Fatty acid desaturase, at 181,927 to 182,868 GFF3362 GFF3363 - BUG/TctC family periplasmic protein, at 183,020 to 184,000 GFF3363 Position (kb) 181 182 183Strain fitness (log2 ratio) -2 -1 0 1 2at 181.045 kb on + strandat 181.045 kb on + strandat 181.045 kb on + strandat 181.046 kb on - strandat 181.046 kb on - strandat 181.046 kb on - strandat 181.136 kb on - strand, within GFF3361at 181.174 kb on + strand, within GFF3361at 181.174 kb on + strand, within GFF3361at 181.246 kb on + strand, within GFF3361at 181.246 kb on + strand, within GFF3361at 181.247 kb on - strand, within GFF3361at 181.247 kb on - strand, within GFF3361at 181.247 kb on - strand, within GFF3361at 181.247 kb on - strand, within GFF3361at 181.247 kb on - strand, within GFF3361at 181.247 kb on - strand, within GFF3361at 181.247 kb on - strand, within GFF3361at 181.369 kb on - strand, within GFF3361at 181.453 kb on + strand, within GFF3361at 181.453 kb on + strand, within GFF3361at 181.454 kb on - strand, within GFF3361at 181.454 kb on - strand, within GFF3361at 181.454 kb on - strand, within GFF3361at 181.454 kb on - strand, within GFF3361at 181.603 kb on + strand, within GFF3361at 181.612 kb on + strand, within GFF3361at 181.928 kb on + strandat 182.051 kb on + strand, within GFF3362at 182.120 kb on + strand, within GFF3362at 182.120 kb on + strand, within GFF3362at 182.120 kb on + strand, within GFF3362at 182.120 kb on + strand, within GFF3362at 182.121 kb on - strand, within GFF3362at 182.121 kb on - strand, within GFF3362at 182.121 kb on - strand, within GFF3362at 182.231 kb on + strand, within GFF3362at 182.240 kb on + strand, within GFF3362at 182.240 kb on + strand, within GFF3362at 182.250 kb on - strand, within GFF3362at 182.285 kb on + strand, within GFF3362at 182.285 kb on + strand, within GFF3362at 182.285 kb on + strand, within GFF3362at 182.286 kb on - strand, within GFF3362at 182.286 kb on - strand, within GFF3362at 182.286 kb on - strand, within GFF3362at 182.288 kb on + strand, within GFF3362at 182.288 kb on + strand, within GFF3362at 182.289 kb on - strand, within GFF3362at 182.289 kb on - strand, within GFF3362at 182.289 kb on - strand, within GFF3362at 182.289 kb on - strand, within GFF3362at 182.444 kb on + strand, within GFF3362at 182.444 kb on + strand, within GFF3362at 182.444 kb on + strand, within GFF3362at 182.444 kb on + strand, within GFF3362at 182.444 kb on + strand, within GFF3362at 182.445 kb on - strand, within GFF3362at 182.445 kb on - strand, within GFF3362at 182.445 kb on - strand, within GFF3362at 182.495 kb on + strand, within GFF3362at 182.534 kb on + strand, within GFF3362at 182.534 kb on + strand, within GFF3362at 182.535 kb on - strand, within GFF3362at 182.535 kb on - strand, within GFF3362at 182.567 kb on + strand, within GFF3362at 182.567 kb on + strand, within GFF3362at 182.762 kb on + strand, within GFF3362at 182.798 kb on + strandat 182.799 kb on - strandat 182.889 kb on + strandat 182.889 kb on + strandat 182.889 kb on + strandat 182.889 kb on + strandat 182.889 kb on + strandat 182.890 kb on - strandat 182.890 kb on - strandat 182.927 kb on + strandat 182.928 kb on - strandat 182.972 kb on + strandat 182.972 kb on + strandat 182.973 kb on - strandat 182.973 kb on - strandat 182.973 kb on - strandat 182.973 kb on - strandat 183.107 kb on + strandat 183.263 kb on + strand, within GFF3363at 183.290 kb on + strand, within GFF3363at 183.290 kb on + strand, within GFF3363at 183.356 kb on - strand, within GFF3363at 183.357 kb on - strand, within GFF3363at 183.357 kb on - strand, within GFF3363at 183.357 kb on - strand, within GFF3363at 183.494 kb on + strand, within GFF3363at 183.494 kb on + strand, within GFF3363at 183.494 kb on + strand, within GFF3363at 183.495 kb on - strand, within GFF3363at 183.515 kb on + strand, within GFF3363at 183.701 kb on + strand, within GFF3363at 183.701 kb on + strand, within GFF3363at 183.701 kb on + strand, within GFF3363at 183.701 kb on + strand, within GFF3363at 183.702 kb on - strand, within GFF3363at 183.783 kb on - strand, within GFF3363

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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181,045 + -0.7
181,045 + -0.5
181,045 + -0.9
181,046 - +1.7
181,046 - -0.8
181,046 - -0.5
181,136 - GFF3361 0.16 -0.5
181,174 + GFF3361 0.20 +0.4
181,174 + GFF3361 0.20 -0.4
181,246 + GFF3361 0.28 +0.9
181,246 + GFF3361 0.28 -0.1
181,247 - GFF3361 0.28 +0.3
181,247 - GFF3361 0.28 +0.3
181,247 - GFF3361 0.28 -0.3
181,247 - GFF3361 0.28 -0.3
181,247 - GFF3361 0.28 +2.2
181,247 - GFF3361 0.28 +0.5
181,247 - GFF3361 0.28 +0.3
181,369 - GFF3361 0.41 -0.1
181,453 + GFF3361 0.50 -0.7
181,453 + GFF3361 0.50 -0.1
181,454 - GFF3361 0.50 -0.9
181,454 - GFF3361 0.50 -0.6
181,454 - GFF3361 0.50 -0.1
181,454 - GFF3361 0.50 -1.7
181,603 + GFF3361 0.66 -0.1
181,612 + GFF3361 0.67 +0.2
181,928 + +0.6
182,051 + GFF3362 0.13 -0.3
182,120 + GFF3362 0.20 +0.7
182,120 + GFF3362 0.20 -0.7
182,120 + GFF3362 0.20 +0.1
182,120 + GFF3362 0.20 +0.3
182,121 - GFF3362 0.21 +1.1
182,121 - GFF3362 0.21 +0.7
182,121 - GFF3362 0.21 -0.1
182,231 + GFF3362 0.32 +0.2
182,240 + GFF3362 0.33 -0.2
182,240 + GFF3362 0.33 -0.6
182,250 - GFF3362 0.34 +0.3
182,285 + GFF3362 0.38 +0.6
182,285 + GFF3362 0.38 -0.9
182,285 + GFF3362 0.38 +0.7
182,286 - GFF3362 0.38 -0.3
182,286 - GFF3362 0.38 -0.2
182,286 - GFF3362 0.38 +0.1
182,288 + GFF3362 0.38 -1.7
182,288 + GFF3362 0.38 +0.5
182,289 - GFF3362 0.38 +0.9
182,289 - GFF3362 0.38 -0.9
182,289 - GFF3362 0.38 +0.8
182,289 - GFF3362 0.38 -2.3
182,444 + GFF3362 0.55 +0.1
182,444 + GFF3362 0.55 +0.5
182,444 + GFF3362 0.55 +0.4
182,444 + GFF3362 0.55 +0.3
182,444 + GFF3362 0.55 -0.7
182,445 - GFF3362 0.55 +0.1
182,445 - GFF3362 0.55 -0.1
182,445 - GFF3362 0.55 +0.3
182,495 + GFF3362 0.60 +0.1
182,534 + GFF3362 0.64 +0.2
182,534 + GFF3362 0.64 +0.7
182,535 - GFF3362 0.65 +1.5
182,535 - GFF3362 0.65 -0.7
182,567 + GFF3362 0.68 -0.9
182,567 + GFF3362 0.68 -0.7
182,762 + GFF3362 0.89 -1.7
182,798 + -1.0
182,799 - -0.5
182,889 + +0.3
182,889 + +1.9
182,889 + -0.1
182,889 + +1.2
182,889 + -0.6
182,890 - -0.3
182,890 - -0.6
182,927 + -1.5
182,928 - -0.8
182,972 + -0.2
182,972 + +0.6
182,973 - -1.2
182,973 - +1.9
182,973 - -0.1
182,973 - -2.2
183,107 + +0.8
183,263 + GFF3363 0.25 -1.2
183,290 + GFF3363 0.28 +0.1
183,290 + GFF3363 0.28 -0.7
183,356 - GFF3363 0.34 -0.7
183,357 - GFF3363 0.34 -1.1
183,357 - GFF3363 0.34 +0.3
183,357 - GFF3363 0.34 -0.4
183,494 + GFF3363 0.48 -0.7
183,494 + GFF3363 0.48 -0.2
183,494 + GFF3363 0.48 -0.5
183,495 - GFF3363 0.48 +0.4
183,515 + GFF3363 0.50 -0.4
183,701 + GFF3363 0.69 +0.7
183,701 + GFF3363 0.69 +1.5
183,701 + GFF3363 0.69 -1.7
183,701 + GFF3363 0.69 -0.7
183,702 - GFF3363 0.70 -0.5
183,783 - GFF3363 0.78 -0.3

Or see this region's nucleotide sequence