Strain Fitness in Variovorax sp. SCN45 around GFF3245

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3244 and GFF3245 overlap by 4 nucleotidesGFF3245 and GFF3246 are separated by 57 nucleotides GFF3244 - Spermidine synthase N-terminal extension / Spermidine synthase (EC 2.5.1.16), at 58,862 to 60,505 GFF3244 GFF3245 - hypothetical protein, at 60,502 to 60,720 GFF3245 GFF3246 - hypothetical protein, at 60,778 to 62,268 GFF3246 Position (kb) 60 61Strain fitness (log2 ratio) -2 -1 0 1 2 3at 59.556 kb on + strand, within GFF3244at 59.556 kb on + strand, within GFF3244at 59.556 kb on + strand, within GFF3244at 59.556 kb on + strand, within GFF3244at 59.557 kb on - strand, within GFF3244at 59.557 kb on - strand, within GFF3244at 59.662 kb on - strand, within GFF3244at 59.752 kb on - strand, within GFF3244at 59.787 kb on + strand, within GFF3244at 59.788 kb on - strand, within GFF3244at 59.788 kb on - strand, within GFF3244at 60.033 kb on + strand, within GFF3244at 60.033 kb on + strand, within GFF3244at 60.033 kb on + strand, within GFF3244at 60.034 kb on - strand, within GFF3244at 60.180 kb on + strand, within GFF3244at 60.181 kb on - strand, within GFF3244at 60.280 kb on - strand, within GFF3244at 60.318 kb on + strand, within GFF3244at 60.318 kb on + strand, within GFF3244at 60.319 kb on - strand, within GFF3244at 60.319 kb on - strand, within GFF3244at 60.319 kb on - strand, within GFF3244at 60.319 kb on - strand, within GFF3244at 60.319 kb on - strand, within GFF3244at 60.366 kb on + strandat 60.366 kb on + strandat 60.366 kb on + strandat 60.367 kb on - strandat 60.367 kb on - strandat 60.396 kb on + strandat 60.397 kb on - strandat 60.608 kb on + strand, within GFF3245at 60.609 kb on - strand, within GFF3245at 60.609 kb on - strand, within GFF3245at 60.609 kb on - strand, within GFF3245at 60.609 kb on - strand, within GFF3245at 60.609 kb on - strand, within GFF3245at 60.671 kb on + strand, within GFF3245at 60.671 kb on + strand, within GFF3245at 60.672 kb on - strand, within GFF3245at 60.736 kb on + strandat 60.736 kb on + strandat 60.736 kb on + strandat 60.736 kb on + strandat 60.737 kb on - strandat 60.737 kb on - strandat 60.737 kb on - strandat 60.739 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.747 kb on + strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.748 kb on - strandat 60.803 kb on + strandat 60.804 kb on - strandat 61.100 kb on + strand, within GFF3246at 61.100 kb on + strand, within GFF3246at 61.101 kb on - strand, within GFF3246at 61.142 kb on + strand, within GFF3246at 61.143 kb on - strand, within GFF3246at 61.143 kb on - strand, within GFF3246at 61.143 kb on - strand, within GFF3246at 61.160 kb on + strand, within GFF3246at 61.160 kb on + strand, within GFF3246at 61.160 kb on + strand, within GFF3246at 61.178 kb on + strand, within GFF3246at 61.179 kb on - strand, within GFF3246at 61.179 kb on - strand, within GFF3246at 61.179 kb on - strand, within GFF3246at 61.179 kb on - strand, within GFF3246at 61.184 kb on + strand, within GFF3246at 61.184 kb on + strand, within GFF3246at 61.184 kb on + strand, within GFF3246at 61.184 kb on + strand, within GFF3246at 61.185 kb on - strand, within GFF3246at 61.185 kb on - strand, within GFF3246at 61.185 kb on - strand, within GFF3246at 61.185 kb on - strand, within GFF3246at 61.185 kb on - strand, within GFF3246at 61.185 kb on - strand, within GFF3246at 61.211 kb on + strand, within GFF3246at 61.211 kb on + strand, within GFF3246at 61.211 kb on + strand, within GFF3246at 61.211 kb on + strand, within GFF3246at 61.211 kb on + strand, within GFF3246at 61.211 kb on + strand, within GFF3246at 61.212 kb on - strand, within GFF3246at 61.322 kb on + strand, within GFF3246at 61.322 kb on + strand, within GFF3246at 61.322 kb on + strand, within GFF3246at 61.323 kb on - strand, within GFF3246at 61.323 kb on - strand, within GFF3246at 61.323 kb on - strand, within GFF3246at 61.323 kb on - strand, within GFF3246at 61.323 kb on - strand, within GFF3246at 61.323 kb on - strand, within GFF3246at 61.331 kb on + strand, within GFF3246at 61.332 kb on - strand, within GFF3246at 61.332 kb on - strand, within GFF3246at 61.332 kb on - strand, within GFF3246at 61.679 kb on + strand, within GFF3246at 61.703 kb on + strand, within GFF3246at 61.704 kb on - strand, within GFF3246

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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59,556 + GFF3244 0.42 -1.0
59,556 + GFF3244 0.42 +0.1
59,556 + GFF3244 0.42 +0.5
59,556 + GFF3244 0.42 -0.6
59,557 - GFF3244 0.42 -0.4
59,557 - GFF3244 0.42 +0.0
59,662 - GFF3244 0.49 -0.2
59,752 - GFF3244 0.54 -0.7
59,787 + GFF3244 0.56 -0.2
59,788 - GFF3244 0.56 +0.2
59,788 - GFF3244 0.56 -0.1
60,033 + GFF3244 0.71 -1.8
60,033 + GFF3244 0.71 -0.9
60,033 + GFF3244 0.71 +0.9
60,034 - GFF3244 0.71 +1.0
60,180 + GFF3244 0.80 -0.9
60,181 - GFF3244 0.80 -0.6
60,280 - GFF3244 0.86 -1.1
60,318 + GFF3244 0.89 -1.4
60,318 + GFF3244 0.89 +0.5
60,319 - GFF3244 0.89 -1.8
60,319 - GFF3244 0.89 -0.9
60,319 - GFF3244 0.89 +1.4
60,319 - GFF3244 0.89 -0.5
60,319 - GFF3244 0.89 -0.2
60,366 + -0.8
60,366 + -0.7
60,366 + -0.6
60,367 - -0.9
60,367 - +2.9
60,396 + -0.7
60,397 - -0.7
60,608 + GFF3245 0.48 +0.4
60,609 - GFF3245 0.49 +0.3
60,609 - GFF3245 0.49 -0.0
60,609 - GFF3245 0.49 -0.4
60,609 - GFF3245 0.49 +0.7
60,609 - GFF3245 0.49 +0.5
60,671 + GFF3245 0.77 +0.4
60,671 + GFF3245 0.77 +0.1
60,672 - GFF3245 0.78 -0.4
60,736 + -0.3
60,736 + -0.3
60,736 + +0.9
60,736 + +0.2
60,737 - -1.0
60,737 - +0.1
60,737 - +0.1
60,739 + -0.4
60,747 + -0.4
60,747 + +0.3
60,747 + -1.0
60,747 + +0.0
60,747 + +0.7
60,747 + -0.2
60,747 + -0.9
60,747 + -0.3
60,747 + +0.3
60,747 + -0.5
60,748 - +0.1
60,748 - -0.1
60,748 - -0.3
60,748 - +1.9
60,748 - -0.4
60,748 - +0.1
60,748 - -0.6
60,748 - +0.6
60,748 - +0.3
60,803 + +0.0
60,804 - -0.1
61,100 + GFF3246 0.22 +0.3
61,100 + GFF3246 0.22 +1.6
61,101 - GFF3246 0.22 +0.2
61,142 + GFF3246 0.24 -0.5
61,143 - GFF3246 0.24 -0.5
61,143 - GFF3246 0.24 -0.2
61,143 - GFF3246 0.24 +0.5
61,160 + GFF3246 0.26 +0.2
61,160 + GFF3246 0.26 -0.7
61,160 + GFF3246 0.26 +1.9
61,178 + GFF3246 0.27 +0.1
61,179 - GFF3246 0.27 +0.5
61,179 - GFF3246 0.27 +0.1
61,179 - GFF3246 0.27 -0.7
61,179 - GFF3246 0.27 -0.5
61,184 + GFF3246 0.27 +0.2
61,184 + GFF3246 0.27 +0.4
61,184 + GFF3246 0.27 -0.7
61,184 + GFF3246 0.27 -0.0
61,185 - GFF3246 0.27 -0.3
61,185 - GFF3246 0.27 +1.8
61,185 - GFF3246 0.27 +0.5
61,185 - GFF3246 0.27 -1.2
61,185 - GFF3246 0.27 -0.8
61,185 - GFF3246 0.27 +0.0
61,211 + GFF3246 0.29 -0.6
61,211 + GFF3246 0.29 +0.2
61,211 + GFF3246 0.29 -0.1
61,211 + GFF3246 0.29 -1.5
61,211 + GFF3246 0.29 -2.3
61,211 + GFF3246 0.29 -0.2
61,212 - GFF3246 0.29 -0.9
61,322 + GFF3246 0.36 -0.4
61,322 + GFF3246 0.36 -0.7
61,322 + GFF3246 0.36 +0.2
61,323 - GFF3246 0.37 -0.1
61,323 - GFF3246 0.37 -0.7
61,323 - GFF3246 0.37 -0.7
61,323 - GFF3246 0.37 +0.3
61,323 - GFF3246 0.37 +1.0
61,323 - GFF3246 0.37 +0.7
61,331 + GFF3246 0.37 +1.7
61,332 - GFF3246 0.37 -0.3
61,332 - GFF3246 0.37 +0.2
61,332 - GFF3246 0.37 -2.7
61,679 + GFF3246 0.60 +1.0
61,703 + GFF3246 0.62 -1.4
61,704 - GFF3246 0.62 -0.5

Or see this region's nucleotide sequence