Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3207 and GFF3208 overlap by 4 nucleotides
GFF3207 - hypothetical protein, at 25,551 to 26,420
GFF3207
GFF3208 - Transposon Tn7 transposition protein tnsB, at 26,417 to 28,687
GFF3208
Position (kb)
25
26
27 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 24.567 kb on - strand at 24.567 kb on - strand at 24.601 kb on - strand at 24.632 kb on - strand at 24.755 kb on + strand at 24.756 kb on - strand at 24.756 kb on - strand at 24.756 kb on - strand at 24.875 kb on + strand at 24.875 kb on + strand at 24.876 kb on - strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.970 kb on + strand at 24.971 kb on - strand at 24.971 kb on - strand at 24.971 kb on - strand at 24.971 kb on - strand at 24.971 kb on - strand at 24.997 kb on + strand at 24.997 kb on + strand at 25.015 kb on - strand at 25.139 kb on + strand at 25.146 kb on + strand at 25.146 kb on + strand at 25.146 kb on + strand at 25.147 kb on - strand at 25.147 kb on - strand at 25.150 kb on + strand at 25.150 kb on + strand at 25.163 kb on - strand at 25.195 kb on + strand at 25.195 kb on + strand at 25.195 kb on + strand at 25.287 kb on - strand at 25.287 kb on - strand at 25.287 kb on - strand at 25.290 kb on + strand at 25.290 kb on + strand at 25.290 kb on + strand at 25.290 kb on + strand at 25.291 kb on - strand at 25.291 kb on - strand at 25.291 kb on - strand at 25.291 kb on - strand at 25.556 kb on + strand at 25.636 kb on - strand at 25.639 kb on + strand, within GFF3207 at 25.640 kb on - strand, within GFF3207 at 25.710 kb on - strand, within GFF3207 at 25.710 kb on - strand, within GFF3207 at 25.723 kb on + strand, within GFF3207 at 25.723 kb on + strand, within GFF3207 at 25.724 kb on - strand, within GFF3207 at 25.724 kb on - strand, within GFF3207 at 25.747 kb on + strand, within GFF3207 at 25.773 kb on + strand, within GFF3207 at 25.952 kb on + strand, within GFF3207 at 25.953 kb on - strand, within GFF3207 at 25.953 kb on - strand, within GFF3207 at 25.953 kb on - strand, within GFF3207 at 25.953 kb on - strand, within GFF3207 at 26.098 kb on - strand, within GFF3207 at 26.142 kb on + strand, within GFF3207 at 26.142 kb on + strand, within GFF3207 at 26.142 kb on + strand, within GFF3207 at 26.143 kb on - strand, within GFF3207 at 26.568 kb on + strand at 26.568 kb on + strand at 26.569 kb on - strand at 26.569 kb on - strand at 26.569 kb on - strand at 26.753 kb on - strand, within GFF3208 at 26.843 kb on - strand, within GFF3208 at 26.864 kb on + strand, within GFF3208 at 26.865 kb on - strand, within GFF3208 at 26.883 kb on - strand, within GFF3208 at 26.918 kb on + strand, within GFF3208 at 26.997 kb on + strand, within GFF3208 at 26.997 kb on + strand, within GFF3208 at 26.997 kb on + strand, within GFF3208 at 26.997 kb on + strand, within GFF3208 at 26.998 kb on - strand, within GFF3208 at 26.998 kb on - strand, within GFF3208 at 26.998 kb on - strand, within GFF3208 at 26.999 kb on + strand, within GFF3208 at 26.999 kb on + strand, within GFF3208 at 26.999 kb on + strand, within GFF3208 at 26.999 kb on + strand, within GFF3208 at 26.999 kb on + strand, within GFF3208 at 26.999 kb on + strand, within GFF3208 at 27.000 kb on - strand, within GFF3208 at 27.000 kb on - strand, within GFF3208 at 27.000 kb on - strand, within GFF3208 at 27.000 kb on - strand, within GFF3208 at 27.000 kb on - strand, within GFF3208 at 27.088 kb on + strand, within GFF3208 at 27.105 kb on - strand, within GFF3208 at 27.217 kb on - strand, within GFF3208 at 27.270 kb on - strand, within GFF3208 at 27.378 kb on + strand, within GFF3208 at 27.378 kb on + strand, within GFF3208 at 27.379 kb on - strand, within GFF3208 at 27.379 kb on - strand, within GFF3208 at 27.398 kb on + strand, within GFF3208
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 24,567 - +0.1 24,567 - +0.8 24,601 - +0.8 24,632 - +0.7 24,755 + -0.9 24,756 - +0.1 24,756 - +1.6 24,756 - -0.9 24,875 + -0.8 24,875 + -0.4 24,876 - -2.3 24,970 + -0.5 24,970 + -0.1 24,970 + -0.9 24,970 + -0.8 24,970 + -0.8 24,970 + -0.3 24,970 + -0.6 24,970 + +0.9 24,970 + -0.9 24,970 + -0.1 24,970 + -0.6 24,970 + -0.5 24,970 + -0.6 24,970 + +1.3 24,970 + +0.1 24,970 + -0.1 24,970 + +0.5 24,970 + +1.3 24,970 + -0.4 24,971 - -0.1 24,971 - -0.2 24,971 - -0.0 24,971 - +0.0 24,971 - +0.3 24,997 + -0.7 24,997 + -0.2 25,015 - -0.3 25,139 + -0.7 25,146 + -1.6 25,146 + -0.6 25,146 + -0.8 25,147 - -0.4 25,147 - -1.8 25,150 + -0.2 25,150 + -0.0 25,163 - -0.3 25,195 + -0.1 25,195 + -0.5 25,195 + -0.5 25,287 - +0.2 25,287 - -0.4 25,287 - +1.9 25,290 + +0.5 25,290 + -1.1 25,290 + -1.3 25,290 + -0.2 25,291 - -0.9 25,291 - -1.2 25,291 - -0.1 25,291 - -0.8 25,556 + +0.2 25,636 - +0.7 25,639 + GFF3207 0.10 -0.8 25,640 - GFF3207 0.10 -1.7 25,710 - GFF3207 0.18 -0.3 25,710 - GFF3207 0.18 +0.6 25,723 + GFF3207 0.20 -0.1 25,723 + GFF3207 0.20 +0.5 25,724 - GFF3207 0.20 -0.4 25,724 - GFF3207 0.20 +0.0 25,747 + GFF3207 0.23 +1.3 25,773 + GFF3207 0.26 -0.6 25,952 + GFF3207 0.46 -0.8 25,953 - GFF3207 0.46 -0.6 25,953 - GFF3207 0.46 -1.0 25,953 - GFF3207 0.46 -0.3 25,953 - GFF3207 0.46 -0.9 26,098 - GFF3207 0.63 -0.1 26,142 + GFF3207 0.68 -1.0 26,142 + GFF3207 0.68 -1.2 26,142 + GFF3207 0.68 +0.3 26,143 - GFF3207 0.68 +0.4 26,568 + +0.3 26,568 + +1.9 26,569 - -0.4 26,569 - +0.4 26,569 - +0.0 26,753 - GFF3208 0.15 -0.1 26,843 - GFF3208 0.19 -0.6 26,864 + GFF3208 0.20 +0.6 26,865 - GFF3208 0.20 -0.2 26,883 - GFF3208 0.21 -0.5 26,918 + GFF3208 0.22 +1.7 26,997 + GFF3208 0.26 -0.3 26,997 + GFF3208 0.26 -0.1 26,997 + GFF3208 0.26 -0.4 26,997 + GFF3208 0.26 -0.8 26,998 - GFF3208 0.26 -0.6 26,998 - GFF3208 0.26 -0.7 26,998 - GFF3208 0.26 -0.2 26,999 + GFF3208 0.26 -0.2 26,999 + GFF3208 0.26 -0.5 26,999 + GFF3208 0.26 -2.1 26,999 + GFF3208 0.26 +2.9 26,999 + GFF3208 0.26 +0.7 26,999 + GFF3208 0.26 +0.7 27,000 - GFF3208 0.26 -0.2 27,000 - GFF3208 0.26 +1.2 27,000 - GFF3208 0.26 -0.7 27,000 - GFF3208 0.26 -0.2 27,000 - GFF3208 0.26 +0.2 27,088 + GFF3208 0.30 +0.2 27,105 - GFF3208 0.30 +0.1 27,217 - GFF3208 0.35 +0.5 27,270 - GFF3208 0.38 +0.7 27,378 + GFF3208 0.42 -0.3 27,378 + GFF3208 0.42 -0.1 27,379 - GFF3208 0.42 +0.2 27,379 - GFF3208 0.42 -0.8 27,398 + GFF3208 0.43 -0.9
Or see this region's nucleotide sequence