Strain Fitness in Variovorax sp. SCN45 around GFF3168

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3167 and GFF3168 are separated by 17 nucleotidesGFF3168 and GFF3169 are separated by 10 nucleotides GFF3167 - BUG/TctC family periplasmic protein, at 606,777 to 607,763 GFF3167 GFF3168 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 607,781 to 609,223 GFF3168 GFF3169 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 609,234 to 610,754 GFF3169 Position (kb) 607 608 609 610Strain fitness (log2 ratio) -1 0 1at 608.400 kb on - strand, within GFF3168at 608.904 kb on - strand, within GFF3168at 608.909 kb on + strand, within GFF3168at 609.528 kb on + strand, within GFF3169at 609.729 kb on + strand, within GFF3169at 609.729 kb on + strand, within GFF3169at 609.729 kb on + strand, within GFF3169at 609.730 kb on - strand, within GFF3169at 609.730 kb on - strand, within GFF3169at 609.799 kb on - strand, within GFF3169at 609.799 kb on - strand, within GFF3169at 609.861 kb on + strand, within GFF3169at 609.862 kb on - strand, within GFF3169at 610.060 kb on + strand, within GFF3169at 610.061 kb on - strand, within GFF3169

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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608,400 - GFF3168 0.43 +0.3
608,904 - GFF3168 0.78 +1.6
608,909 + GFF3168 0.78 +1.6
609,528 + GFF3169 0.19 -0.0
609,729 + GFF3169 0.33 -1.5
609,729 + GFF3169 0.33 -0.1
609,729 + GFF3169 0.33 +0.4
609,730 - GFF3169 0.33 -0.4
609,730 - GFF3169 0.33 +0.1
609,799 - GFF3169 0.37 -0.9
609,799 - GFF3169 0.37 -0.8
609,861 + GFF3169 0.41 -0.4
609,862 - GFF3169 0.41 -1.2
610,060 + GFF3169 0.54 -0.6
610,061 - GFF3169 0.54 +0.5

Or see this region's nucleotide sequence