Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3157 and GFF3158 are separated by 4 nucleotides GFF3158 and GFF3159 are separated by 110 nucleotides GFF3159 and GFF3160 are separated by 34 nucleotides
GFF3157 - Sulfite dehydrogenase (quinone), membrane-anchor subunit SoeC (EC 1.8.5.6), at 593,344 to 594,276
GFF3157
GFF3158 - Sulfite dehydrogenase (quinone), iron-sulfur subunit SoeB (EC 1.8.5.6), at 594,281 to 595,009
GFF3158
GFF3159 - Sulfite dehydrogenase (quinone), molybdopterin-containing subunit SoeA (EC 1.8.5.6), at 595,120 to 598,065
GFF3159
GFF3160 - Transcriptional regulator YeiE, LysR family, at 598,100 to 599,089
GFF3160
Position (kb) 595
596
597
598
599 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 594.325 kb on - strand at 594.325 kb on - strand at 594.499 kb on - strand, within GFF3158 at 594.612 kb on + strand, within GFF3158 at 594.753 kb on + strand, within GFF3158 at 594.798 kb on + strand, within GFF3158 at 594.956 kb on - strand at 595.145 kb on + strand at 595.212 kb on - strand at 595.212 kb on - strand at 595.470 kb on - strand, within GFF3159 at 595.470 kb on - strand, within GFF3159 at 595.571 kb on + strand, within GFF3159 at 595.571 kb on + strand, within GFF3159 at 595.572 kb on - strand, within GFF3159 at 595.572 kb on - strand, within GFF3159 at 595.625 kb on + strand, within GFF3159 at 595.625 kb on + strand, within GFF3159 at 595.626 kb on - strand, within GFF3159 at 595.626 kb on - strand, within GFF3159 at 595.727 kb on + strand, within GFF3159 at 595.728 kb on - strand, within GFF3159 at 595.821 kb on - strand, within GFF3159 at 595.884 kb on - strand, within GFF3159 at 595.907 kb on + strand, within GFF3159 at 595.907 kb on + strand, within GFF3159 at 595.907 kb on + strand, within GFF3159 at 595.908 kb on - strand, within GFF3159 at 595.908 kb on - strand, within GFF3159 at 595.931 kb on + strand, within GFF3159 at 595.932 kb on - strand, within GFF3159 at 595.958 kb on + strand, within GFF3159 at 595.958 kb on + strand, within GFF3159 at 595.958 kb on + strand, within GFF3159 at 596.078 kb on + strand, within GFF3159 at 596.087 kb on + strand, within GFF3159 at 596.087 kb on + strand, within GFF3159 at 596.277 kb on - strand, within GFF3159 at 597.056 kb on + strand, within GFF3159 at 597.283 kb on + strand, within GFF3159 at 597.284 kb on - strand, within GFF3159 at 597.554 kb on + strand, within GFF3159 at 597.555 kb on - strand, within GFF3159 at 597.605 kb on + strand, within GFF3159 at 597.606 kb on - strand, within GFF3159 at 597.974 kb on + strand at 597.974 kb on + strand at 597.974 kb on + strand at 598.137 kb on + strand at 598.137 kb on + strand at 598.356 kb on + strand, within GFF3160 at 598.490 kb on + strand, within GFF3160 at 598.491 kb on - strand, within GFF3160 at 598.972 kb on - strand, within GFF3160
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 594,325 - -0.9 594,325 - -0.0 594,499 - GFF3158 0.30 -2.1 594,612 + GFF3158 0.45 -0.6 594,753 + GFF3158 0.65 -3.3 594,798 + GFF3158 0.71 +0.3 594,956 - +0.3 595,145 + -0.4 595,212 - -0.2 595,212 - -1.0 595,470 - GFF3159 0.12 -1.8 595,470 - GFF3159 0.12 -1.6 595,571 + GFF3159 0.15 +0.3 595,571 + GFF3159 0.15 -1.4 595,572 - GFF3159 0.15 -0.6 595,572 - GFF3159 0.15 -1.0 595,625 + GFF3159 0.17 +0.2 595,625 + GFF3159 0.17 -0.3 595,626 - GFF3159 0.17 +0.2 595,626 - GFF3159 0.17 +0.2 595,727 + GFF3159 0.21 +0.2 595,728 - GFF3159 0.21 -0.8 595,821 - GFF3159 0.24 +0.7 595,884 - GFF3159 0.26 +0.5 595,907 + GFF3159 0.27 +0.2 595,907 + GFF3159 0.27 +0.1 595,907 + GFF3159 0.27 -1.0 595,908 - GFF3159 0.27 -1.1 595,908 - GFF3159 0.27 -1.2 595,931 + GFF3159 0.28 +0.2 595,932 - GFF3159 0.28 -0.1 595,958 + GFF3159 0.28 -0.4 595,958 + GFF3159 0.28 -1.0 595,958 + GFF3159 0.28 -0.3 596,078 + GFF3159 0.33 -1.1 596,087 + GFF3159 0.33 +0.2 596,087 + GFF3159 0.33 +0.3 596,277 - GFF3159 0.39 -0.3 597,056 + GFF3159 0.66 -0.7 597,283 + GFF3159 0.73 -2.0 597,284 - GFF3159 0.73 -0.5 597,554 + GFF3159 0.83 -0.1 597,555 - GFF3159 0.83 -1.8 597,605 + GFF3159 0.84 -1.0 597,606 - GFF3159 0.84 +0.3 597,974 + +0.5 597,974 + +0.8 597,974 + -0.4 598,137 + -0.2 598,137 + -1.0 598,356 + GFF3160 0.26 +0.5 598,490 + GFF3160 0.39 +0.6 598,491 - GFF3160 0.39 -0.1 598,972 - GFF3160 0.88 +0.0
Or see this region's nucleotide sequence