Strain Fitness in Variovorax sp. SCN45 around GFF3142

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3141 and GFF3142 are separated by 9 nucleotidesGFF3142 and GFF3143 are separated by 128 nucleotides GFF3141 - Phosphate acetyltransferase (EC 2.3.1.8), at 572,244 to 573,197 GFF3141 GFF3142 - Indolepyruvate ferredoxin oxidoreductase, alpha and beta subunits, at 573,207 to 576,818 GFF3142 GFF3143 - hypothetical protein, at 576,947 to 577,816 GFF3143 Position (kb) 573 574 575 576 577Strain fitness (log2 ratio) -2 -1 0 1 2at 572.212 kb on - strandat 572.277 kb on + strandat 572.277 kb on + strandat 572.278 kb on - strandat 573.704 kb on - strand, within GFF3142at 573.733 kb on + strand, within GFF3142at 573.734 kb on - strand, within GFF3142at 573.734 kb on - strand, within GFF3142at 574.111 kb on + strand, within GFF3142at 574.228 kb on - strand, within GFF3142at 574.588 kb on + strand, within GFF3142at 574.589 kb on - strand, within GFF3142at 574.589 kb on - strand, within GFF3142at 574.589 kb on - strand, within GFF3142at 574.741 kb on + strand, within GFF3142at 574.742 kb on - strand, within GFF3142at 574.945 kb on + strand, within GFF3142at 574.945 kb on + strand, within GFF3142at 574.946 kb on - strand, within GFF3142at 574.946 kb on - strand, within GFF3142at 575.056 kb on + strand, within GFF3142at 575.198 kb on - strand, within GFF3142at 575.198 kb on - strand, within GFF3142at 575.198 kb on - strand, within GFF3142at 575.254 kb on + strand, within GFF3142at 575.254 kb on + strand, within GFF3142at 575.255 kb on - strand, within GFF3142at 575.255 kb on - strand, within GFF3142at 575.257 kb on + strand, within GFF3142at 575.257 kb on + strand, within GFF3142at 575.257 kb on + strand, within GFF3142at 575.258 kb on - strand, within GFF3142at 575.258 kb on - strand, within GFF3142at 575.258 kb on - strand, within GFF3142at 575.467 kb on + strand, within GFF3142at 575.468 kb on - strand, within GFF3142at 575.704 kb on + strand, within GFF3142at 575.793 kb on - strand, within GFF3142at 575.830 kb on + strand, within GFF3142at 576.232 kb on + strand, within GFF3142at 576.232 kb on + strand, within GFF3142at 576.232 kb on + strand, within GFF3142at 576.232 kb on + strand, within GFF3142at 576.233 kb on - strand, within GFF3142at 576.256 kb on + strand, within GFF3142at 576.256 kb on + strand, within GFF3142at 576.454 kb on + strand, within GFF3142at 576.913 kb on + strandat 576.914 kb on - strandat 577.021 kb on - strandat 577.038 kb on + strand, within GFF3143at 577.101 kb on + strand, within GFF3143at 577.102 kb on - strand, within GFF3143at 577.102 kb on - strand, within GFF3143at 577.147 kb on - strand, within GFF3143at 577.218 kb on + strand, within GFF3143at 577.219 kb on - strand, within GFF3143at 577.263 kb on + strand, within GFF3143at 577.263 kb on + strand, within GFF3143at 577.264 kb on - strand, within GFF3143at 577.324 kb on - strand, within GFF3143at 577.324 kb on - strand, within GFF3143at 577.443 kb on + strand, within GFF3143at 577.519 kb on + strand, within GFF3143at 577.519 kb on + strand, within GFF3143at 577.519 kb on + strand, within GFF3143at 577.602 kb on + strand, within GFF3143at 577.602 kb on + strand, within GFF3143at 577.602 kb on + strand, within GFF3143at 577.602 kb on + strand, within GFF3143at 577.603 kb on - strand, within GFF3143at 577.603 kb on - strand, within GFF3143at 577.603 kb on - strand, within GFF3143at 577.603 kb on - strand, within GFF3143at 577.603 kb on - strand, within GFF3143at 577.603 kb on - strand, within GFF3143at 577.603 kb on - strand, within GFF3143at 577.691 kb on + strand, within GFF3143at 577.691 kb on + strand, within GFF3143

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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572,212 - -0.8
572,277 + -0.2
572,277 + -0.7
572,278 - -1.1
573,704 - GFF3142 0.14 -0.4
573,733 + GFF3142 0.15 -0.4
573,734 - GFF3142 0.15 +0.4
573,734 - GFF3142 0.15 +0.3
574,111 + GFF3142 0.25 -1.3
574,228 - GFF3142 0.28 -0.2
574,588 + GFF3142 0.38 -0.5
574,589 - GFF3142 0.38 +0.1
574,589 - GFF3142 0.38 -0.2
574,589 - GFF3142 0.38 +0.4
574,741 + GFF3142 0.42 +0.5
574,742 - GFF3142 0.42 +0.3
574,945 + GFF3142 0.48 -1.2
574,945 + GFF3142 0.48 +0.0
574,946 - GFF3142 0.48 +0.9
574,946 - GFF3142 0.48 -0.3
575,056 + GFF3142 0.51 -0.5
575,198 - GFF3142 0.55 +1.2
575,198 - GFF3142 0.55 -1.4
575,198 - GFF3142 0.55 +0.3
575,254 + GFF3142 0.57 +0.4
575,254 + GFF3142 0.57 -0.7
575,255 - GFF3142 0.57 -0.6
575,255 - GFF3142 0.57 +0.8
575,257 + GFF3142 0.57 -0.5
575,257 + GFF3142 0.57 +1.3
575,257 + GFF3142 0.57 -0.5
575,258 - GFF3142 0.57 -0.2
575,258 - GFF3142 0.57 +0.2
575,258 - GFF3142 0.57 +1.0
575,467 + GFF3142 0.63 +0.1
575,468 - GFF3142 0.63 -1.2
575,704 + GFF3142 0.69 +0.9
575,793 - GFF3142 0.72 -0.2
575,830 + GFF3142 0.73 -0.5
576,232 + GFF3142 0.84 +0.3
576,232 + GFF3142 0.84 -1.5
576,232 + GFF3142 0.84 +0.4
576,232 + GFF3142 0.84 +0.2
576,233 - GFF3142 0.84 -1.4
576,256 + GFF3142 0.84 +0.2
576,256 + GFF3142 0.84 -0.6
576,454 + GFF3142 0.90 +0.0
576,913 + +0.0
576,914 - -1.7
577,021 - -1.0
577,038 + GFF3143 0.10 +0.6
577,101 + GFF3143 0.18 +0.6
577,102 - GFF3143 0.18 +0.2
577,102 - GFF3143 0.18 -1.3
577,147 - GFF3143 0.23 +0.3
577,218 + GFF3143 0.31 -0.9
577,219 - GFF3143 0.31 +1.0
577,263 + GFF3143 0.36 -0.4
577,263 + GFF3143 0.36 -1.0
577,264 - GFF3143 0.36 +2.4
577,324 - GFF3143 0.43 -1.4
577,324 - GFF3143 0.43 -0.3
577,443 + GFF3143 0.57 -0.4
577,519 + GFF3143 0.66 +0.6
577,519 + GFF3143 0.66 -0.2
577,519 + GFF3143 0.66 -0.3
577,602 + GFF3143 0.75 +0.4
577,602 + GFF3143 0.75 -0.1
577,602 + GFF3143 0.75 -0.4
577,602 + GFF3143 0.75 -0.7
577,603 - GFF3143 0.75 -0.2
577,603 - GFF3143 0.75 -0.1
577,603 - GFF3143 0.75 +0.3
577,603 - GFF3143 0.75 -1.9
577,603 - GFF3143 0.75 -1.2
577,603 - GFF3143 0.75 -0.9
577,603 - GFF3143 0.75 +1.2
577,691 + GFF3143 0.86 -0.9
577,691 + GFF3143 0.86 +0.4

Or see this region's nucleotide sequence