Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3063 and GFF3064 are separated by 137 nucleotides GFF3064 and GFF3065 are separated by 4 nucleotides GFF3065 and GFF3066 are separated by 46 nucleotides
GFF3063 - mandelate racemase/muconate lactonizing enzyme family protein, at 496,691 to 497,857
GFF3063
GFF3064 - Exodeoxyribonuclease III (EC 3.1.11.2), at 497,995 to 498,780
GFF3064
GFF3065 - hypothetical protein, at 498,785 to 499,336
GFF3065
GFF3066 - Nitrogen regulation protein NR(I), GlnG (=NtrC), at 499,383 to 500,918
GFF3066
Position (kb) 498
499
500 Strain fitness (log2 ratio) -2
-1
0
1 at 497.856 kb on - strand at 497.856 kb on - strand at 497.892 kb on + strand at 497.892 kb on + strand at 497.893 kb on - strand at 497.893 kb on - strand at 497.942 kb on + strand at 497.942 kb on + strand at 497.943 kb on - strand at 497.943 kb on - strand at 497.943 kb on - strand at 497.943 kb on - strand at 498.002 kb on + strand at 498.002 kb on + strand at 498.002 kb on + strand at 498.002 kb on + strand at 498.002 kb on + strand at 498.003 kb on - strand at 498.003 kb on - strand at 498.006 kb on + strand at 498.006 kb on + strand at 498.006 kb on + strand at 498.007 kb on - strand at 498.007 kb on - strand at 498.007 kb on - strand at 498.007 kb on - strand at 498.007 kb on - strand at 498.007 kb on - strand at 498.358 kb on + strand, within GFF3064 at 498.359 kb on - strand, within GFF3064 at 498.359 kb on - strand, within GFF3064 at 498.610 kb on + strand, within GFF3064 at 498.610 kb on + strand, within GFF3064 at 498.611 kb on - strand, within GFF3064 at 498.611 kb on - strand, within GFF3064 at 499.043 kb on + strand, within GFF3065 at 499.043 kb on + strand, within GFF3065 at 499.043 kb on + strand, within GFF3065 at 499.043 kb on + strand, within GFF3065 at 499.044 kb on - strand, within GFF3065 at 499.044 kb on - strand, within GFF3065 at 499.044 kb on - strand, within GFF3065 at 499.052 kb on + strand, within GFF3065 at 499.053 kb on - strand, within GFF3065 at 499.053 kb on - strand, within GFF3065 at 499.067 kb on + strand, within GFF3065 at 499.067 kb on + strand, within GFF3065 at 499.067 kb on + strand, within GFF3065 at 499.067 kb on + strand, within GFF3065 at 499.068 kb on - strand, within GFF3065 at 499.068 kb on - strand, within GFF3065 at 499.068 kb on - strand, within GFF3065 at 499.094 kb on + strand, within GFF3065 at 499.094 kb on + strand, within GFF3065 at 499.095 kb on - strand, within GFF3065 at 499.095 kb on - strand, within GFF3065 at 499.259 kb on + strand, within GFF3065 at 499.259 kb on + strand, within GFF3065 at 499.259 kb on + strand, within GFF3065 at 499.259 kb on + strand, within GFF3065 at 499.259 kb on + strand, within GFF3065 at 499.260 kb on - strand, within GFF3065 at 499.260 kb on - strand, within GFF3065 at 499.260 kb on - strand, within GFF3065 at 499.260 kb on - strand, within GFF3065 at 499.260 kb on - strand, within GFF3065 at 499.335 kb on - strand at 500.128 kb on + strand, within GFF3066
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 497,856 - +0.7 497,856 - -0.8 497,892 + -0.7 497,892 + +0.4 497,893 - +0.4 497,893 - +0.2 497,942 + +0.1 497,942 + +0.3 497,943 - +0.1 497,943 - -1.2 497,943 - -0.6 497,943 - -0.8 498,002 + -0.8 498,002 + -0.3 498,002 + +0.3 498,002 + +0.2 498,002 + -0.3 498,003 - +0.0 498,003 - +0.2 498,006 + +1.6 498,006 + -0.9 498,006 + +0.1 498,007 - -0.5 498,007 - -0.0 498,007 - -2.2 498,007 - +0.1 498,007 - +0.8 498,007 - -0.9 498,358 + GFF3064 0.46 -1.0 498,359 - GFF3064 0.46 -0.5 498,359 - GFF3064 0.46 +0.6 498,610 + GFF3064 0.78 +1.6 498,610 + GFF3064 0.78 -0.3 498,611 - GFF3064 0.78 -2.1 498,611 - GFF3064 0.78 -1.3 499,043 + GFF3065 0.47 -0.8 499,043 + GFF3065 0.47 +0.0 499,043 + GFF3065 0.47 +0.2 499,043 + GFF3065 0.47 -1.3 499,044 - GFF3065 0.47 +0.2 499,044 - GFF3065 0.47 -1.0 499,044 - GFF3065 0.47 -2.1 499,052 + GFF3065 0.48 -0.8 499,053 - GFF3065 0.49 +0.5 499,053 - GFF3065 0.49 +0.3 499,067 + GFF3065 0.51 -0.3 499,067 + GFF3065 0.51 +0.2 499,067 + GFF3065 0.51 -0.6 499,067 + GFF3065 0.51 -1.9 499,068 - GFF3065 0.51 +0.5 499,068 - GFF3065 0.51 -0.5 499,068 - GFF3065 0.51 +0.0 499,094 + GFF3065 0.56 +0.3 499,094 + GFF3065 0.56 -0.1 499,095 - GFF3065 0.56 +0.0 499,095 - GFF3065 0.56 -1.2 499,259 + GFF3065 0.86 -0.7 499,259 + GFF3065 0.86 +0.0 499,259 + GFF3065 0.86 -1.4 499,259 + GFF3065 0.86 +1.0 499,259 + GFF3065 0.86 -0.9 499,260 - GFF3065 0.86 +0.2 499,260 - GFF3065 0.86 -1.0 499,260 - GFF3065 0.86 -1.5 499,260 - GFF3065 0.86 -2.3 499,260 - GFF3065 0.86 -0.9 499,335 - +0.1 500,128 + GFF3066 0.49 +0.3
Or see this region's nucleotide sequence