Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2994 and GFF2995 are separated by 6 nucleotides GFF2995 and GFF2996 are separated by 14 nucleotides GFF2996 and GFF2997 are separated by 113 nucleotides GFF2997 and GFF2998 are separated by 2 nucleotides
GFF2994 - Transcriptional regulator, LysR family, at 425,660 to 426,610
GFF2994
GFF2995 - hypothetical protein, at 426,617 to 427,279
GFF2995
GFF2996 - Thiol-disulfide isomerase and thioredoxins, at 427,294 to 427,677
GFF2996
GFF2997 - FIG027190: Putative transmembrane protein, at 427,791 to 428,129
GFF2997
GFF2998 - Virulence factor BrkB, at 428,132 to 429,343
GFF2998
Position (kb) 426
427
428 Strain fitness (log2 ratio) -2
-1
0
1
2 at 425.625 kb on + strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.748 kb on - strand at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.141 kb on + strand, within GFF2994 at 426.142 kb on - strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.315 kb on - strand, within GFF2994 at 426.784 kb on - strand, within GFF2995 at 426.784 kb on - strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.015 kb on - strand, within GFF2995 at 427.015 kb on - strand, within GFF2995 at 427.015 kb on - strand, within GFF2995 at 427.029 kb on + strand, within GFF2995 at 427.029 kb on + strand, within GFF2995 at 427.030 kb on - strand, within GFF2995 at 427.030 kb on - strand, within GFF2995 at 427.030 kb on - strand, within GFF2995 at 427.116 kb on + strand, within GFF2995 at 427.116 kb on + strand, within GFF2995 at 427.117 kb on - strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.185 kb on - strand, within GFF2995 at 427.736 kb on + strand at 427.736 kb on + strand at 427.736 kb on + strand at 427.736 kb on + strand at 427.736 kb on + strand at 427.737 kb on - strand at 427.857 kb on + strand, within GFF2997 at 427.857 kb on + strand, within GFF2997 at 427.857 kb on + strand, within GFF2997 at 427.857 kb on + strand, within GFF2997 at 427.858 kb on - strand, within GFF2997 at 427.858 kb on - strand, within GFF2997 at 427.866 kb on + strand, within GFF2997 at 427.866 kb on + strand, within GFF2997 at 427.867 kb on - strand, within GFF2997 at 428.067 kb on + strand, within GFF2997 at 428.068 kb on - strand, within GFF2997 at 428.068 kb on - strand, within GFF2997 at 428.068 kb on - strand, within GFF2997 at 428.094 kb on + strand, within GFF2997 at 428.094 kb on + strand, within GFF2997 at 428.094 kb on + strand, within GFF2997 at 428.127 kb on + strand at 428.127 kb on + strand at 428.127 kb on + strand at 428.127 kb on + strand at 428.127 kb on + strand at 428.128 kb on - strand at 428.128 kb on - strand at 428.128 kb on - strand at 428.128 kb on - strand at 428.134 kb on - strand at 428.134 kb on - strand at 428.134 kb on - strand at 428.271 kb on + strand, within GFF2998 at 428.271 kb on + strand, within GFF2998 at 428.271 kb on + strand, within GFF2998 at 428.271 kb on + strand, within GFF2998 at 428.272 kb on - strand, within GFF2998 at 428.272 kb on - strand, within GFF2998
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 425,625 + +1.6 425,626 - -1.2 425,626 - -0.4 425,626 - -0.7 425,626 - +0.6 425,747 + -0.1 425,747 + +1.6 425,747 + +0.2 425,747 + +0.2 425,747 + -0.1 425,747 + +1.0 425,747 + +1.2 425,747 + -2.1 425,747 + -0.2 425,747 + -1.9 425,748 - +0.0 426,110 + GFF2994 0.47 -0.8 426,110 + GFF2994 0.47 +0.0 426,110 + GFF2994 0.47 -0.2 426,110 + GFF2994 0.47 -1.8 426,110 + GFF2994 0.47 +1.1 426,111 - GFF2994 0.47 -1.2 426,111 - GFF2994 0.47 -0.5 426,111 - GFF2994 0.47 -0.7 426,111 - GFF2994 0.47 +0.6 426,111 - GFF2994 0.47 -1.0 426,111 - GFF2994 0.47 -0.2 426,141 + GFF2994 0.51 +0.2 426,142 - GFF2994 0.51 +0.9 426,167 + GFF2994 0.53 +0.4 426,167 + GFF2994 0.53 -0.1 426,167 + GFF2994 0.53 -0.7 426,168 - GFF2994 0.53 -0.7 426,168 - GFF2994 0.53 -0.7 426,168 - GFF2994 0.53 -0.6 426,315 - GFF2994 0.69 +0.1 426,784 - GFF2995 0.25 +1.5 426,784 - GFF2995 0.25 -0.7 426,843 + GFF2995 0.34 -0.1 426,843 + GFF2995 0.34 -1.7 426,843 + GFF2995 0.34 +0.1 426,843 + GFF2995 0.34 +0.1 426,843 + GFF2995 0.34 -0.4 426,843 + GFF2995 0.34 +0.6 426,843 + GFF2995 0.34 -0.9 426,843 + GFF2995 0.34 +1.0 426,843 + GFF2995 0.34 -0.6 426,843 + GFF2995 0.34 +0.2 426,844 - GFF2995 0.34 -2.2 426,844 - GFF2995 0.34 +0.8 426,844 - GFF2995 0.34 +0.2 426,844 - GFF2995 0.34 -0.4 426,844 - GFF2995 0.34 +0.3 426,844 - GFF2995 0.34 -1.6 426,844 - GFF2995 0.34 -0.6 426,844 - GFF2995 0.34 -1.0 426,844 - GFF2995 0.34 -0.3 426,951 + GFF2995 0.50 -0.2 426,951 + GFF2995 0.50 -0.8 426,951 + GFF2995 0.50 -0.5 426,951 + GFF2995 0.50 -1.8 426,951 + GFF2995 0.50 +2.2 426,951 + GFF2995 0.50 -0.7 426,951 + GFF2995 0.50 -0.3 426,951 + GFF2995 0.50 -0.8 426,952 - GFF2995 0.51 -1.2 426,952 - GFF2995 0.51 -0.4 426,952 - GFF2995 0.51 -1.0 426,952 - GFF2995 0.51 -0.9 426,952 - GFF2995 0.51 -0.0 426,952 - GFF2995 0.51 -0.6 426,952 - GFF2995 0.51 +1.3 426,952 - GFF2995 0.51 -1.0 427,014 + GFF2995 0.60 +0.5 427,014 + GFF2995 0.60 -0.4 427,014 + GFF2995 0.60 +1.3 427,014 + GFF2995 0.60 -0.6 427,014 + GFF2995 0.60 -0.1 427,015 - GFF2995 0.60 -0.1 427,015 - GFF2995 0.60 +0.6 427,015 - GFF2995 0.60 -1.1 427,029 + GFF2995 0.62 -0.4 427,029 + GFF2995 0.62 -1.0 427,030 - GFF2995 0.62 -0.1 427,030 - GFF2995 0.62 -0.8 427,030 - GFF2995 0.62 -0.0 427,116 + GFF2995 0.75 -1.8 427,116 + GFF2995 0.75 -0.9 427,117 - GFF2995 0.75 -0.9 427,122 + GFF2995 0.76 -0.2 427,122 + GFF2995 0.76 +0.4 427,122 + GFF2995 0.76 -0.6 427,122 + GFF2995 0.76 +0.8 427,122 + GFF2995 0.76 -0.7 427,122 + GFF2995 0.76 -0.3 427,122 + GFF2995 0.76 -1.5 427,122 + GFF2995 0.76 -0.6 427,122 + GFF2995 0.76 -0.7 427,122 + GFF2995 0.76 -0.8 427,122 + GFF2995 0.76 -1.0 427,122 + GFF2995 0.76 +0.3 427,122 + GFF2995 0.76 -0.7 427,122 + GFF2995 0.76 +0.6 427,122 + GFF2995 0.76 +1.0 427,122 + GFF2995 0.76 +0.1 427,122 + GFF2995 0.76 +0.8 427,122 + GFF2995 0.76 +0.8 427,122 + GFF2995 0.76 -0.8 427,122 + GFF2995 0.76 -0.7 427,123 - GFF2995 0.76 -0.7 427,123 - GFF2995 0.76 -0.1 427,123 - GFF2995 0.76 -0.4 427,123 - GFF2995 0.76 +0.6 427,123 - GFF2995 0.76 +0.8 427,123 - GFF2995 0.76 +0.4 427,123 - GFF2995 0.76 -0.8 427,123 - GFF2995 0.76 -0.2 427,123 - GFF2995 0.76 +0.3 427,123 - GFF2995 0.76 +0.0 427,123 - GFF2995 0.76 -0.9 427,123 - GFF2995 0.76 -0.5 427,123 - GFF2995 0.76 -0.2 427,123 - GFF2995 0.76 -1.0 427,123 - GFF2995 0.76 +0.3 427,123 - GFF2995 0.76 -0.6 427,123 - GFF2995 0.76 +0.0 427,184 + GFF2995 0.86 +0.0 427,184 + GFF2995 0.86 +0.0 427,184 + GFF2995 0.86 +0.2 427,185 - GFF2995 0.86 -1.5 427,736 + -0.1 427,736 + -1.2 427,736 + +1.3 427,736 + -0.8 427,736 + +0.3 427,737 - +0.8 427,857 + GFF2997 0.19 +0.6 427,857 + GFF2997 0.19 +1.9 427,857 + GFF2997 0.19 -2.7 427,857 + GFF2997 0.19 -0.2 427,858 - GFF2997 0.20 +0.1 427,858 - GFF2997 0.20 -1.0 427,866 + GFF2997 0.22 -0.4 427,866 + GFF2997 0.22 -1.0 427,867 - GFF2997 0.22 +0.3 428,067 + GFF2997 0.81 +0.5 428,068 - GFF2997 0.82 -0.4 428,068 - GFF2997 0.82 -0.5 428,068 - GFF2997 0.82 +0.5 428,094 + GFF2997 0.89 -0.4 428,094 + GFF2997 0.89 +0.2 428,094 + GFF2997 0.89 -0.1 428,127 + +0.8 428,127 + -0.4 428,127 + -2.5 428,127 + -1.2 428,127 + +0.1 428,128 - -0.7 428,128 - +0.3 428,128 - -1.2 428,128 - +0.0 428,134 - -0.1 428,134 - -0.1 428,134 - -0.1 428,271 + GFF2998 0.11 -0.4 428,271 + GFF2998 0.11 -0.0 428,271 + GFF2998 0.11 +0.1 428,271 + GFF2998 0.11 -0.8 428,272 - GFF2998 0.12 -0.3 428,272 - GFF2998 0.12 -1.8
Or see this region's nucleotide sequence