Strain Fitness in Variovorax sp. SCN45 around GFF2979

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2976 and GFF2977 overlap by 4 nucleotidesGFF2977 and GFF2978 are separated by 7 nucleotidesGFF2978 and GFF2979 are separated by 0 nucleotidesGFF2979 and GFF2980 are separated by 81 nucleotides GFF2976 - hypothetical protein, at 407,552 to 408,604 GFF2976 GFF2977 - hypothetical protein, at 408,601 to 409,242 GFF2977 GFF2978 - Aromatic ring-cleaving dioxygenase, at 409,250 to 409,597 GFF2978 GFF2979 - Glutathione S-transferase (EC 2.5.1.18), at 409,598 to 410,254 GFF2979 GFF2980 - Intracellular PHB depolymerase PhaZ2, at 410,336 to 411,556 GFF2980 Position (kb) 409 410 411Strain fitness (log2 ratio) -2 -1 0 1at 408.874 kb on + strand, within GFF2977at 408.970 kb on + strand, within GFF2977at 408.971 kb on - strand, within GFF2977at 408.971 kb on - strand, within GFF2977at 409.046 kb on - strand, within GFF2977at 409.108 kb on + strand, within GFF2977at 409.108 kb on + strand, within GFF2977at 409.108 kb on + strand, within GFF2977at 409.109 kb on - strand, within GFF2977at 409.275 kb on - strandat 409.275 kb on - strandat 409.283 kb on + strandat 409.298 kb on + strand, within GFF2978at 409.298 kb on + strand, within GFF2978at 409.299 kb on - strand, within GFF2978at 409.299 kb on - strand, within GFF2978at 409.299 kb on - strand, within GFF2978at 409.595 kb on + strandat 409.599 kb on + strandat 409.599 kb on + strandat 409.599 kb on + strandat 409.599 kb on + strandat 409.600 kb on - strandat 409.600 kb on - strandat 409.600 kb on - strandat 409.695 kb on + strand, within GFF2979at 409.728 kb on + strand, within GFF2979at 409.729 kb on - strand, within GFF2979at 409.729 kb on - strand, within GFF2979at 409.758 kb on + strand, within GFF2979at 409.758 kb on + strand, within GFF2979at 409.758 kb on + strand, within GFF2979at 409.759 kb on - strand, within GFF2979at 409.759 kb on - strand, within GFF2979at 409.809 kb on + strand, within GFF2979at 409.809 kb on + strand, within GFF2979at 409.810 kb on - strand, within GFF2979at 409.810 kb on - strand, within GFF2979at 410.031 kb on + strand, within GFF2979at 410.217 kb on + strandat 410.217 kb on + strandat 410.217 kb on + strandat 410.218 kb on - strandat 410.218 kb on - strandat 410.351 kb on + strandat 410.399 kb on + strandat 410.399 kb on + strandat 410.399 kb on + strandat 410.399 kb on + strandat 410.400 kb on - strandat 410.400 kb on - strandat 410.717 kb on + strand, within GFF2980at 410.717 kb on + strand, within GFF2980at 410.783 kb on + strand, within GFF2980at 410.783 kb on + strand, within GFF2980at 410.784 kb on - strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.140 kb on + strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.141 kb on - strand, within GFF2980at 411.194 kb on + strand, within GFF2980at 411.195 kb on - strand, within GFF2980at 411.195 kb on - strand, within GFF2980at 411.195 kb on - strand, within GFF2980at 411.195 kb on - strand, within GFF2980at 411.203 kb on + strand, within GFF2980at 411.203 kb on + strand, within GFF2980at 411.204 kb on - strand, within GFF2980at 411.204 kb on - strand, within GFF2980at 411.236 kb on + strand, within GFF2980at 411.236 kb on + strand, within GFF2980at 411.236 kb on + strand, within GFF2980at 411.237 kb on - strand, within GFF2980at 411.237 kb on - strand, within GFF2980

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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408,874 + GFF2977 0.43 -1.1
408,970 + GFF2977 0.57 -0.6
408,971 - GFF2977 0.58 -1.8
408,971 - GFF2977 0.58 +0.1
409,046 - GFF2977 0.69 +0.4
409,108 + GFF2977 0.79 -0.2
409,108 + GFF2977 0.79 -0.5
409,108 + GFF2977 0.79 -0.7
409,109 - GFF2977 0.79 +0.1
409,275 - +0.5
409,275 - +0.3
409,283 + +0.4
409,298 + GFF2978 0.14 -0.9
409,298 + GFF2978 0.14 -1.8
409,299 - GFF2978 0.14 -0.4
409,299 - GFF2978 0.14 +0.1
409,299 - GFF2978 0.14 +0.4
409,595 + -0.9
409,599 + -1.0
409,599 + +0.0
409,599 + -0.2
409,599 + -0.7
409,600 - -0.6
409,600 - -1.4
409,600 - -0.2
409,695 + GFF2979 0.15 -0.3
409,728 + GFF2979 0.20 -0.7
409,729 - GFF2979 0.20 -0.9
409,729 - GFF2979 0.20 +0.8
409,758 + GFF2979 0.24 -0.1
409,758 + GFF2979 0.24 -1.0
409,758 + GFF2979 0.24 -0.2
409,759 - GFF2979 0.25 -1.0
409,759 - GFF2979 0.25 -1.8
409,809 + GFF2979 0.32 +1.0
409,809 + GFF2979 0.32 -0.9
409,810 - GFF2979 0.32 -0.1
409,810 - GFF2979 0.32 +0.2
410,031 + GFF2979 0.66 +0.8
410,217 + +0.0
410,217 + -0.4
410,217 + -1.0
410,218 - -0.4
410,218 - -1.1
410,351 + -0.7
410,399 + +0.2
410,399 + -0.3
410,399 + +0.6
410,399 + -0.1
410,400 - +0.3
410,400 - +1.3
410,717 + GFF2980 0.31 -0.5
410,717 + GFF2980 0.31 +0.6
410,783 + GFF2980 0.37 +1.0
410,783 + GFF2980 0.37 +0.7
410,784 - GFF2980 0.37 -1.1
411,140 + GFF2980 0.66 +0.4
411,140 + GFF2980 0.66 -0.6
411,140 + GFF2980 0.66 -0.6
411,140 + GFF2980 0.66 -0.6
411,140 + GFF2980 0.66 -0.5
411,140 + GFF2980 0.66 -0.1
411,140 + GFF2980 0.66 -0.7
411,140 + GFF2980 0.66 +0.4
411,140 + GFF2980 0.66 -0.3
411,140 + GFF2980 0.66 -0.1
411,140 + GFF2980 0.66 +0.9
411,140 + GFF2980 0.66 -0.1
411,140 + GFF2980 0.66 -1.4
411,140 + GFF2980 0.66 -1.2
411,140 + GFF2980 0.66 -1.7
411,140 + GFF2980 0.66 -0.3
411,140 + GFF2980 0.66 -0.3
411,140 + GFF2980 0.66 -0.5
411,140 + GFF2980 0.66 -0.2
411,140 + GFF2980 0.66 -0.2
411,140 + GFF2980 0.66 -1.4
411,140 + GFF2980 0.66 -1.6
411,141 - GFF2980 0.66 -0.9
411,141 - GFF2980 0.66 -1.1
411,141 - GFF2980 0.66 +0.4
411,141 - GFF2980 0.66 +0.4
411,141 - GFF2980 0.66 -0.0
411,141 - GFF2980 0.66 -0.6
411,141 - GFF2980 0.66 +0.3
411,141 - GFF2980 0.66 -0.4
411,141 - GFF2980 0.66 -0.5
411,141 - GFF2980 0.66 -1.2
411,141 - GFF2980 0.66 -0.2
411,141 - GFF2980 0.66 -1.8
411,141 - GFF2980 0.66 -1.2
411,141 - GFF2980 0.66 -0.7
411,141 - GFF2980 0.66 -0.5
411,141 - GFF2980 0.66 +0.4
411,141 - GFF2980 0.66 -2.1
411,141 - GFF2980 0.66 +0.3
411,141 - GFF2980 0.66 -0.9
411,141 - GFF2980 0.66 -2.0
411,141 - GFF2980 0.66 -1.5
411,141 - GFF2980 0.66 +1.6
411,141 - GFF2980 0.66 -0.4
411,141 - GFF2980 0.66 -0.4
411,141 - GFF2980 0.66 -1.5
411,141 - GFF2980 0.66 -0.4
411,141 - GFF2980 0.66 -0.4
411,141 - GFF2980 0.66 -0.1
411,141 - GFF2980 0.66 +0.8
411,194 + GFF2980 0.70 +0.0
411,195 - GFF2980 0.70 -1.1
411,195 - GFF2980 0.70 -1.0
411,195 - GFF2980 0.70 +0.0
411,195 - GFF2980 0.70 -1.1
411,203 + GFF2980 0.71 -0.1
411,203 + GFF2980 0.71 -0.4
411,204 - GFF2980 0.71 +0.0
411,204 - GFF2980 0.71 +0.1
411,236 + GFF2980 0.74 +0.2
411,236 + GFF2980 0.74 -0.2
411,236 + GFF2980 0.74 +0.7
411,237 - GFF2980 0.74 -2.0
411,237 - GFF2980 0.74 +0.8

Or see this region's nucleotide sequence