Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2808 and GFF2809 are separated by 13 nucleotides GFF2809 and GFF2810 overlap by 4 nucleotides GFF2810 and GFF2811 overlap by 1 nucleotides GFF2811 and GFF2812 are separated by 15 nucleotides
GFF2808 - hypothetical protein, at 237,593 to 238,042
GFF2808
GFF2809 - Putative transcription regulator, at 238,056 to 238,505
GFF2809
GFF2810 - hypothetical protein, at 238,502 to 238,975
GFF2810
GFF2811 - Transcriptional regulator, YafY family, at 238,975 to 239,685
GFF2811
GFF2812 - Replicative DNA helicase (DnaB) (EC 3.6.4.12), at 239,701 to 241,107
GFF2812
Position (kb) 238
239
240 Strain fitness (log2 ratio) -2
-1
0
1
2 at 238.032 kb on + strand at 238.032 kb on + strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.033 kb on - strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.053 kb on + strand at 238.054 kb on - strand at 238.054 kb on - strand at 238.054 kb on - strand at 238.054 kb on - strand at 238.192 kb on - strand, within GFF2809 at 238.192 kb on - strand, within GFF2809 at 238.192 kb on - strand, within GFF2809 at 238.192 kb on - strand, within GFF2809 at 238.239 kb on + strand, within GFF2809 at 238.239 kb on + strand, within GFF2809 at 238.248 kb on + strand, within GFF2809 at 238.249 kb on - strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.338 kb on + strand, within GFF2809 at 238.339 kb on - strand, within GFF2809 at 238.339 kb on - strand, within GFF2809 at 238.339 kb on - strand, within GFF2809 at 238.339 kb on - strand, within GFF2809 at 238.339 kb on - strand, within GFF2809 at 238.339 kb on - strand, within GFF2809 at 238.339 kb on - strand, within GFF2809 at 238.407 kb on + strand, within GFF2809 at 238.408 kb on - strand, within GFF2809 at 238.408 kb on - strand, within GFF2809 at 238.440 kb on + strand, within GFF2809 at 238.441 kb on - strand, within GFF2809 at 238.461 kb on + strand at 238.461 kb on + strand at 238.461 kb on + strand at 238.461 kb on + strand at 238.462 kb on - strand at 238.462 kb on - strand at 238.462 kb on - strand at 238.462 kb on - strand at 238.462 kb on - strand at 238.462 kb on - strand at 238.462 kb on - strand at 238.462 kb on - strand at 238.480 kb on + strand at 238.480 kb on + strand at 238.480 kb on + strand at 238.480 kb on + strand at 238.481 kb on - strand at 238.481 kb on - strand at 238.541 kb on + strand at 238.541 kb on + strand at 238.541 kb on + strand at 238.541 kb on + strand at 238.542 kb on - strand at 238.542 kb on - strand at 238.542 kb on - strand at 238.542 kb on - strand at 238.542 kb on - strand at 238.542 kb on - strand at 238.789 kb on + strand, within GFF2810 at 238.789 kb on + strand, within GFF2810 at 238.790 kb on - strand, within GFF2810 at 238.857 kb on - strand, within GFF2810 at 238.931 kb on + strand at 238.932 kb on - strand at 238.932 kb on - strand at 238.932 kb on - strand at 238.932 kb on - strand at 238.932 kb on - strand at 239.083 kb on + strand, within GFF2811 at 239.084 kb on - strand, within GFF2811 at 239.084 kb on - strand, within GFF2811 at 239.341 kb on + strand, within GFF2811 at 239.341 kb on + strand, within GFF2811 at 239.341 kb on + strand, within GFF2811 at 239.341 kb on + strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.342 kb on - strand, within GFF2811 at 239.434 kb on + strand, within GFF2811 at 239.491 kb on + strand, within GFF2811 at 239.491 kb on + strand, within GFF2811 at 239.491 kb on + strand, within GFF2811 at 239.491 kb on + strand, within GFF2811 at 239.491 kb on + strand, within GFF2811 at 239.492 kb on - strand, within GFF2811 at 239.492 kb on - strand, within GFF2811 at 239.642 kb on - strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.683 kb on + strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.684 kb on - strand at 239.706 kb on - strand at 239.706 kb on - strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.723 kb on + strand at 239.724 kb on - strand at 239.724 kb on - strand at 239.724 kb on - strand at 239.724 kb on - strand at 239.724 kb on - strand at 239.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 238,032 + +0.2 238,032 + -0.5 238,033 - +0.3 238,033 - +1.4 238,033 - -0.7 238,033 - -1.7 238,033 - +1.2 238,033 - +0.4 238,033 - +0.2 238,033 - -0.6 238,033 - +0.0 238,053 + -0.4 238,053 + +0.0 238,053 + +0.1 238,053 + -0.6 238,053 + -0.2 238,053 + +0.3 238,053 + -1.0 238,053 + +0.0 238,053 + -0.1 238,053 + +0.1 238,053 + +0.6 238,053 + -0.4 238,054 - +0.8 238,054 - +0.6 238,054 - +0.2 238,054 - -1.6 238,192 - GFF2809 0.30 +0.6 238,192 - GFF2809 0.30 +2.0 238,192 - GFF2809 0.30 -0.3 238,192 - GFF2809 0.30 -1.1 238,239 + GFF2809 0.41 -0.2 238,239 + GFF2809 0.41 +1.4 238,248 + GFF2809 0.43 +0.8 238,249 - GFF2809 0.43 +1.6 238,338 + GFF2809 0.63 -0.4 238,338 + GFF2809 0.63 -1.5 238,338 + GFF2809 0.63 +0.3 238,338 + GFF2809 0.63 +0.5 238,338 + GFF2809 0.63 -1.2 238,338 + GFF2809 0.63 -1.4 238,338 + GFF2809 0.63 +0.2 238,338 + GFF2809 0.63 -0.1 238,339 - GFF2809 0.63 +0.1 238,339 - GFF2809 0.63 -0.3 238,339 - GFF2809 0.63 +0.6 238,339 - GFF2809 0.63 -1.6 238,339 - GFF2809 0.63 +0.6 238,339 - GFF2809 0.63 -0.4 238,339 - GFF2809 0.63 -0.3 238,407 + GFF2809 0.78 -1.2 238,408 - GFF2809 0.78 -0.2 238,408 - GFF2809 0.78 -0.7 238,440 + GFF2809 0.85 -0.5 238,441 - GFF2809 0.86 +0.0 238,461 + -0.2 238,461 + -1.0 238,461 + -0.4 238,461 + -0.0 238,462 - +0.6 238,462 - +0.7 238,462 - +0.0 238,462 - -0.2 238,462 - +0.3 238,462 - -1.0 238,462 - +0.5 238,462 - +0.2 238,480 + -1.4 238,480 + +0.0 238,480 + -0.3 238,480 + +0.6 238,481 - +0.0 238,481 - -0.9 238,541 + -1.7 238,541 + -0.0 238,541 + -1.3 238,541 + +2.6 238,542 - +0.1 238,542 - +0.1 238,542 - -0.3 238,542 - -0.4 238,542 - +0.2 238,542 - +0.2 238,789 + GFF2810 0.61 -0.1 238,789 + GFF2810 0.61 +0.8 238,790 - GFF2810 0.61 +0.1 238,857 - GFF2810 0.75 +2.0 238,931 + -0.9 238,932 - +0.6 238,932 - -0.1 238,932 - -0.5 238,932 - -0.3 238,932 - +0.3 239,083 + GFF2811 0.15 -0.1 239,084 - GFF2811 0.15 +0.1 239,084 - GFF2811 0.15 -1.7 239,341 + GFF2811 0.51 -2.1 239,341 + GFF2811 0.51 +0.6 239,341 + GFF2811 0.51 -1.0 239,341 + GFF2811 0.51 +1.1 239,342 - GFF2811 0.52 +0.6 239,342 - GFF2811 0.52 -0.2 239,342 - GFF2811 0.52 +2.3 239,342 - GFF2811 0.52 +0.1 239,342 - GFF2811 0.52 +0.1 239,342 - GFF2811 0.52 -0.6 239,342 - GFF2811 0.52 +1.4 239,342 - GFF2811 0.52 +0.4 239,434 + GFF2811 0.65 -0.4 239,491 + GFF2811 0.73 -0.6 239,491 + GFF2811 0.73 +0.9 239,491 + GFF2811 0.73 -0.4 239,491 + GFF2811 0.73 -1.6 239,491 + GFF2811 0.73 +1.2 239,492 - GFF2811 0.73 +1.0 239,492 - GFF2811 0.73 -0.3 239,642 - -0.7 239,683 + +0.6 239,683 + +0.2 239,683 + +0.3 239,683 + -0.1 239,683 + +0.3 239,683 + -0.7 239,683 + -2.6 239,683 + +0.0 239,683 + +0.4 239,683 + -0.6 239,684 - -0.1 239,684 - -0.8 239,684 - +1.4 239,684 - -0.3 239,684 - +0.0 239,684 - -0.8 239,684 - -0.7 239,684 - +0.3 239,684 - +1.6 239,684 - +0.5 239,684 - -0.2 239,706 - -0.3 239,706 - +1.7 239,723 + +0.1 239,723 + +0.4 239,723 + +0.4 239,723 + -0.8 239,723 + +0.7 239,723 + -0.1 239,723 + -1.0 239,723 + -1.4 239,723 + +2.2 239,724 - -0.3 239,724 - -0.0 239,724 - +1.9 239,724 - -0.9 239,724 - +0.1 239,724 - +1.6
Or see this region's nucleotide sequence