Strain Fitness in Variovorax sp. SCN45 around GFF2728

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
161,152	+		GFF2727	0.33	+0.3
161,152	+		GFF2727	0.33	-1.5
161,152	+		GFF2727	0.33	+0.4
161,153	-		GFF2727	0.33	-0.9
161,153	-		GFF2727	0.33	-0.1
161,153	-		GFF2727	0.33	-1.1
161,153	-		GFF2727	0.33	-1.2
161,287	+		GFF2727	0.45	-0.8
161,288	-		GFF2727	0.45	+0.5
161,288	-		GFF2727	0.45	+0.1
161,317	+		GFF2727	0.48	+0.4
161,317	+		GFF2727	0.48	+0.0
161,317	+		GFF2727	0.48	+0.3
161,317	+		GFF2727	0.48	+2.2
161,317	+		GFF2727	0.48	-0.2
161,318	-		GFF2727	0.48	+0.7
161,318	-		GFF2727	0.48	+0.2
161,318	-		GFF2727	0.48	+1.0
161,318	-		GFF2727	0.48	+0.4
161,318	-		GFF2727	0.48	-2.5
161,341	+		GFF2727	0.50	-0.7
161,554	+		GFF2727	0.69	-0.1
161,554	+		GFF2727	0.69	-0.1
161,554	+		GFF2727	0.69	-0.3
161,554	+		GFF2727	0.69	-0.6
161,555	-		GFF2727	0.69	+0.4
161,555	-		GFF2727	0.69	-0.6
161,645	-		GFF2727	0.77	-1.0
161,875	-				+0.2
161,875	-				-0.9
161,875	-				-0.1
161,937	+				-0.5
161,937	+				-0.3
161,937	+				-1.8
161,937	+				-0.7
161,938	-				+1.0
161,938	-				+0.2
161,938	-				-0.4
161,938	-				+0.3
161,938	-				-0.3
161,938	-				+0.3
161,938	-				-1.3
161,939	+				-0.4
161,939	+				+0.6
161,939	+				-0.4
161,939	+				+0.5
161,939	+				-0.4
161,939	+				+1.9
161,939	+				-0.3
161,939	+				-0.6
161,939	+				+0.4
161,939	+				+1.2
161,939	+				+0.0
161,939	+				-0.6
161,939	+				-0.5
161,940	-				-0.4
161,940	-				-0.8
161,940	-				+0.3
161,940	-				+1.6
161,940	-				+0.5
161,940	-				-1.1
161,940	-				+0.2
161,940	-				-0.1
161,940	-				+0.9
161,940	-				+0.2
161,940	-				+0.0
161,940	-				-0.2
161,940	-				-0.5
161,940	-				-0.5
161,940	-				-1.2
161,973	+				+0.3
161,973	+				+0.0
161,973	+				-1.4
161,973	+				+0.2
161,973	+				+1.0
161,974	-				+0.1
161,974	-				-0.7
161,974	-				+1.0
161,974	-				-1.9
161,974	-				-2.0
162,017	+				-1.4
162,017	+				-0.3
162,017	+				-0.7
162,196	+		GFF2728	0.34	-1.7
162,196	+		GFF2728	0.34	-0.3
162,197	-		GFF2728	0.34	+0.3
162,197	-		GFF2728	0.34	-0.4
162,197	-		GFF2728	0.34	+1.1
162,197	-		GFF2728	0.34	+1.6
162,197	-		GFF2728	0.34	+0.2
162,537	+				-0.1
162,666	+				+1.3
162,666	+				-1.1
162,667	-				-0.6
162,667	-				-0.7
162,667	-				-1.1
162,667	-				+0.2
162,667	-				+0.7
162,667	-				-1.3
162,667	-				-1.6
162,726	+				-0.4
162,761	+				+0.0
162,761	+				+0.0
162,761	+				-0.3
162,761	+				+0.1
162,761	+				-1.2
162,762	-				-0.5
162,765	+				-0.6
162,765	+				-0.1
162,765	+				+0.0
162,765	+				+0.0
162,765	+				-0.2
162,765	+				-0.6
162,765	+				+0.1
162,765	+				+0.6
162,765	+				-0.5
162,765	+				+0.6
162,765	+				+0.5
162,765	+				-0.4
162,765	+				-0.7
162,765	+				+0.0
162,765	+				-0.4
162,765	+				-1.0
162,765	+				-0.4
162,766	-				+0.6
162,766	-				-1.3
162,766	-				-0.4
162,766	-				+1.8
162,766	-				-2.4
162,766	-				+0.2
162,766	-				-1.2
162,766	-				+0.0
162,766	-				-0.6
162,766	-				-1.1
162,766	-				-0.1
162,766	-				-0.2
162,766	-				-0.3
162,766	-				+0.3
162,766	-				+0.0
162,766	-				+0.4
162,766	-				-1.1
162,792	+				-0.8
162,792	+				-0.3
162,792	+				+0.4
162,793	-				-0.4
162,793	-				+0.4
162,793	-				-1.0
162,862	-				+0.5
162,862	-				-0.3
162,870	+				-1.1
162,870	+				-0.7
162,870	+				-0.4
162,870	+				-0.5
162,870	+				+1.6
162,870	+				+1.6
162,870	+				-0.0
162,871	-				-0.4
162,871	-				+0.3
162,871	-				-0.4
162,871	-				-0.7
162,871	-				-0.7
162,871	-				-0.2
162,871	-				+0.5
162,871	-				+0.3
162,871	-				+1.0
162,918	+				+0.6
162,918	+				+2.2
162,918	+				+0.1
162,918	+				-0.2
162,919	-				+1.9
162,919	-				+1.9
162,933	+				-1.1
162,933	+				-1.0
162,951	+				-1.5
162,952	-				-0.4
162,952	-				+0.9
163,132	+				-1.0
163,133	-				+0.1
163,204	+				+1.4
163,205	-				+0.5
163,254	+				+0.1
163,254	+				-0.8
163,255	-				-0.7
163,286	+				+0.1
163,286	+				+0.1
163,374	+				-1.1
163,374	+				+1.0
163,374	+				-0.1
163,374	+				+0.4
163,374	+				+0.8
163,375	-				-0.3
163,375	-				+3.2
163,375	-				+0.5
163,560	+				-0.4
163,560	+				-0.4
163,561	-				-0.3