Strain Fitness in Variovorax sp. SCN45 around GFF2661

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2659 and GFF2660 are separated by 2 nucleotidesGFF2660 and GFF2661 are separated by 136 nucleotidesGFF2661 and GFF2662 are separated by 39 nucleotides GFF2659 - Electron transfer flavoprotein, alpha subunit, at 81,412 to 82,344 GFF2659 GFF2660 - Electron transfer flavoprotein, beta subunit, at 82,347 to 83,096 GFF2660 GFF2661 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 83,233 to 84,513 GFF2661 GFF2662 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 84,553 to 85,506 GFF2662 Position (kb) 83 84 85Strain fitness (log2 ratio) -2 -1 0 1at 83.151 kb on + strandat 83.152 kb on - strandat 83.152 kb on - strandat 83.152 kb on - strandat 83.152 kb on - strandat 83.234 kb on + strandat 83.234 kb on + strandat 83.234 kb on + strandat 83.236 kb on + strandat 83.236 kb on + strandat 83.236 kb on + strandat 83.237 kb on - strandat 83.237 kb on - strandat 83.237 kb on - strandat 83.237 kb on - strandat 83.310 kb on + strandat 83.310 kb on + strandat 83.310 kb on + strandat 83.311 kb on - strandat 83.311 kb on - strandat 83.311 kb on - strandat 83.538 kb on - strand, within GFF2661at 83.667 kb on - strand, within GFF2661at 83.769 kb on - strand, within GFF2661at 83.769 kb on - strand, within GFF2661at 83.769 kb on - strand, within GFF2661at 83.974 kb on - strand, within GFF2661at 84.554 kb on + strandat 84.554 kb on + strandat 84.554 kb on + strandat 84.555 kb on - strandat 84.615 kb on - strandat 84.845 kb on + strand, within GFF2662at 84.899 kb on + strand, within GFF2662at 84.899 kb on + strand, within GFF2662at 84.900 kb on - strand, within GFF2662at 84.905 kb on + strand, within GFF2662at 84.905 kb on + strand, within GFF2662at 84.906 kb on - strand, within GFF2662at 84.906 kb on - strand, within GFF2662at 84.906 kb on - strand, within GFF2662at 84.906 kb on - strand, within GFF2662at 84.906 kb on - strand, within GFF2662at 85.490 kb on + strandat 85.490 kb on + strandat 85.491 kb on - strandat 85.491 kb on - strandat 85.491 kb on - strandat 85.491 kb on - strandat 85.491 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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83,151 + -1.5
83,152 - -0.1
83,152 - +0.6
83,152 - +0.7
83,152 - -0.3
83,234 + +0.7
83,234 + -1.9
83,234 + -0.6
83,236 + +0.6
83,236 + -1.3
83,236 + -0.2
83,237 - -0.9
83,237 - +0.3
83,237 - -0.1
83,237 - -0.1
83,310 + -0.0
83,310 + -0.8
83,310 + -0.6
83,311 - -0.7
83,311 - +0.5
83,311 - +1.3
83,538 - GFF2661 0.24 -0.0
83,667 - GFF2661 0.34 +1.6
83,769 - GFF2661 0.42 +0.4
83,769 - GFF2661 0.42 +0.3
83,769 - GFF2661 0.42 +1.2
83,974 - GFF2661 0.58 -0.4
84,554 + -1.4
84,554 + -0.1
84,554 + -1.5
84,555 - +0.1
84,615 - -0.0
84,845 + GFF2662 0.31 -0.1
84,899 + GFF2662 0.36 -0.7
84,899 + GFF2662 0.36 -0.6
84,900 - GFF2662 0.36 +0.6
84,905 + GFF2662 0.37 -0.3
84,905 + GFF2662 0.37 -0.6
84,906 - GFF2662 0.37 -1.1
84,906 - GFF2662 0.37 -0.6
84,906 - GFF2662 0.37 -1.3
84,906 - GFF2662 0.37 -0.6
84,906 - GFF2662 0.37 +1.0
85,490 + -0.6
85,490 + -0.5
85,491 - -1.3
85,491 - -1.1
85,491 - -0.7
85,491 - -1.7
85,491 - -0.5

Or see this region's nucleotide sequence