Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2653 and GFF2654 are separated by 47 nucleotides GFF2654 and GFF2655 overlap by 4 nucleotides
GFF2653 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 72,116 to 73,729
GFF2653
GFF2654 - Efflux ABC transporter for glutathione/L-cysteine, essential for assembly of bd-type respiratory oxidases => CydC subunit, at 73,777 to 75,492
GFF2654
GFF2655 - Efflux ABC transporter for glutathione/L-cysteine, essential for assembly of bd-type respiratory oxidases => CydD subunit, at 75,489 to 77,177
GFF2655
Position (kb)
73
74
75
76 Strain fitness (log2 ratio)
-2
-1
0
1 at 72.876 kb on + strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 73.111 kb on - strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.153 kb on - strand, within GFF2653 at 73.153 kb on - strand, within GFF2653 at 73.457 kb on - strand, within GFF2653 at 73.477 kb on - strand, within GFF2653 at 73.479 kb on + strand, within GFF2653 at 73.480 kb on - strand, within GFF2653 at 73.608 kb on + strand at 73.609 kb on - strand at 73.674 kb on + strand at 73.675 kb on - strand at 73.675 kb on - strand at 73.745 kb on + strand at 73.745 kb on + strand at 73.746 kb on - strand at 73.746 kb on - strand at 73.746 kb on - strand at 74.583 kb on + strand, within GFF2654 at 75.054 kb on - strand, within GFF2654 at 75.054 kb on - strand, within GFF2654 at 75.054 kb on - strand, within GFF2654 at 75.094 kb on + strand, within GFF2654 at 75.094 kb on + strand, within GFF2654 at 75.221 kb on + strand, within GFF2654 at 75.378 kb on + strand at 75.378 kb on + strand at 75.378 kb on + strand at 75.379 kb on - strand at 75.379 kb on - strand at 76.281 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 72,876 + GFF2653 0.47 -1.1 72,876 + GFF2653 0.47 +0.0 72,876 + GFF2653 0.47 +1.3 73,111 - GFF2653 0.62 -0.3 73,152 + GFF2653 0.64 +0.1 73,152 + GFF2653 0.64 -1.4 73,152 + GFF2653 0.64 +1.2 73,153 - GFF2653 0.64 +0.3 73,153 - GFF2653 0.64 -0.5 73,457 - GFF2653 0.83 -0.5 73,477 - GFF2653 0.84 +0.5 73,479 + GFF2653 0.84 +0.9 73,480 - GFF2653 0.85 -0.4 73,608 + -0.6 73,609 - +0.2 73,674 + -0.1 73,675 - -0.7 73,675 - -0.1 73,745 + -0.7 73,745 + -0.5 73,746 - +1.1 73,746 - -1.0 73,746 - +0.1 74,583 + GFF2654 0.47 -0.5 75,054 - GFF2654 0.74 -1.3 75,054 - GFF2654 0.74 -0.2 75,054 - GFF2654 0.74 -0.2 75,094 + GFF2654 0.77 -1.3 75,094 + GFF2654 0.77 -1.2 75,221 + GFF2654 0.84 -0.8 75,378 + +0.2 75,378 + -0.8 75,378 + +0.1 75,379 - -0.3 75,379 - -0.5 76,281 + GFF2655 0.47 -0.7 76,402 + GFF2655 0.54 +0.7 76,402 + GFF2655 0.54 -1.6 76,402 + GFF2655 0.54 +0.9 76,402 + GFF2655 0.54 +0.5 76,402 + GFF2655 0.54 -0.5 76,402 + GFF2655 0.54 +0.5 76,402 + GFF2655 0.54 +1.0 76,403 - GFF2655 0.54 -0.3 76,403 - GFF2655 0.54 -0.4 76,403 - GFF2655 0.54 -2.4 76,403 - GFF2655 0.54 -1.7 76,403 - GFF2655 0.54 -0.7 76,403 - GFF2655 0.54 -2.1 76,403 - GFF2655 0.54 -0.1 76,403 - GFF2655 0.54 -0.7
Or see this region's nucleotide sequence