Strain Fitness in Variovorax sp. SCN45 around GFF2406

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2404 and GFF2405 are separated by 152 nucleotidesGFF2405 and GFF2406 are separated by 21 nucleotidesGFF2406 and GFF2407 are separated by 8 nucleotidesGFF2407 and GFF2408 are separated by 12 nucleotides GFF2404 - Hypothetical protein with Rieske (2Fe-2S) region, at 456,540 to 457,622 GFF2404 GFF2405 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 457,775 to 458,284 GFF2405 GFF2406 - DipZ protein, at 458,306 to 460,159 GFF2406 GFF2407 - hypothetical protein, at 460,168 to 460,629 GFF2407 GFF2408 - FIG00976241: hypothetical protein, at 460,642 to 461,388 GFF2408 Position (kb) 458 459 460 461Strain fitness (log2 ratio) -2 -1 0 1 2at 457.354 kb on + strand, within GFF2404at 457.354 kb on + strand, within GFF2404at 457.354 kb on + strand, within GFF2404at 457.355 kb on - strand, within GFF2404at 457.355 kb on - strand, within GFF2404at 457.355 kb on - strand, within GFF2404at 457.355 kb on - strand, within GFF2404at 457.449 kb on + strand, within GFF2404at 457.449 kb on + strand, within GFF2404at 457.449 kb on + strand, within GFF2404at 457.450 kb on - strand, within GFF2404at 457.450 kb on - strand, within GFF2404at 457.450 kb on - strand, within GFF2404at 457.710 kb on + strandat 457.717 kb on + strandat 457.717 kb on + strandat 457.718 kb on - strandat 457.718 kb on - strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.727 kb on + strandat 457.728 kb on - strandat 457.728 kb on - strandat 457.728 kb on - strandat 457.728 kb on - strandat 457.728 kb on - strandat 457.729 kb on + strandat 457.729 kb on + strandat 457.730 kb on - strandat 457.730 kb on - strandat 457.731 kb on + strandat 457.731 kb on + strandat 457.731 kb on + strandat 457.731 kb on + strandat 457.731 kb on + strandat 457.732 kb on - strandat 457.732 kb on - strandat 457.946 kb on + strand, within GFF2405at 457.947 kb on - strand, within GFF2405at 457.947 kb on - strand, within GFF2405at 458.237 kb on + strandat 458.328 kb on - strandat 458.810 kb on + strand, within GFF2406at 458.853 kb on + strand, within GFF2406at 458.854 kb on - strand, within GFF2406at 458.854 kb on - strand, within GFF2406at 459.305 kb on + strand, within GFF2406at 459.338 kb on + strand, within GFF2406at 459.338 kb on + strand, within GFF2406at 459.339 kb on - strand, within GFF2406at 459.363 kb on - strand, within GFF2406at 459.363 kb on - strand, within GFF2406at 459.468 kb on - strand, within GFF2406at 459.479 kb on - strand, within GFF2406at 459.491 kb on + strand, within GFF2406at 459.491 kb on + strand, within GFF2406at 459.492 kb on - strand, within GFF2406at 459.492 kb on - strand, within GFF2406at 459.492 kb on - strand, within GFF2406at 459.537 kb on - strand, within GFF2406at 459.591 kb on - strand, within GFF2406at 459.729 kb on - strand, within GFF2406at 459.797 kb on + strand, within GFF2406at 459.797 kb on + strand, within GFF2406at 459.798 kb on - strand, within GFF2406at 459.798 kb on - strand, within GFF2406at 460.182 kb on - strandat 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.333 kb on + strand, within GFF2407at 460.334 kb on - strand, within GFF2407at 460.334 kb on - strand, within GFF2407at 460.334 kb on - strand, within GFF2407at 460.334 kb on - strand, within GFF2407at 460.334 kb on - strand, within GFF2407at 460.468 kb on + strand, within GFF2407at 460.468 kb on + strand, within GFF2407at 460.469 kb on - strand, within GFF2407at 460.505 kb on - strand, within GFF2407at 461.042 kb on + strand, within GFF2408at 461.043 kb on - strand, within GFF2408at 461.043 kb on - strand, within GFF2408

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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457,354 + GFF2404 0.75 +0.3
457,354 + GFF2404 0.75 -0.0
457,354 + GFF2404 0.75 +0.0
457,355 - GFF2404 0.75 +1.0
457,355 - GFF2404 0.75 +0.2
457,355 - GFF2404 0.75 -1.7
457,355 - GFF2404 0.75 -0.5
457,449 + GFF2404 0.84 +0.0
457,449 + GFF2404 0.84 +2.2
457,449 + GFF2404 0.84 -0.5
457,450 - GFF2404 0.84 -0.8
457,450 - GFF2404 0.84 -1.7
457,450 - GFF2404 0.84 -0.3
457,710 + +0.6
457,717 + -0.4
457,717 + -0.8
457,718 - -0.5
457,718 - +0.2
457,727 + +0.0
457,727 + -1.3
457,727 + +0.2
457,727 + +0.2
457,727 + +0.0
457,727 + -0.3
457,727 + -0.6
457,727 + +0.0
457,727 + -1.0
457,727 + -0.4
457,727 + -0.0
457,727 + -1.0
457,728 - -0.5
457,728 - +0.0
457,728 - +0.2
457,728 - -0.5
457,728 - -1.0
457,729 + +0.2
457,729 + -0.9
457,730 - -0.7
457,730 - -0.8
457,731 + -0.5
457,731 + -0.9
457,731 + +0.8
457,731 + +1.0
457,731 + -0.5
457,732 - -0.5
457,732 - -0.6
457,946 + GFF2405 0.34 +2.1
457,947 - GFF2405 0.34 +0.2
457,947 - GFF2405 0.34 -0.7
458,237 + -1.8
458,328 - -0.4
458,810 + GFF2406 0.27 -0.5
458,853 + GFF2406 0.30 -0.5
458,854 - GFF2406 0.30 -0.5
458,854 - GFF2406 0.30 -0.5
459,305 + GFF2406 0.54 +0.4
459,338 + GFF2406 0.56 +0.2
459,338 + GFF2406 0.56 -0.4
459,339 - GFF2406 0.56 +0.8
459,363 - GFF2406 0.57 +0.5
459,363 - GFF2406 0.57 -0.5
459,468 - GFF2406 0.63 +1.2
459,479 - GFF2406 0.63 +1.1
459,491 + GFF2406 0.64 +0.2
459,491 + GFF2406 0.64 -0.3
459,492 - GFF2406 0.64 -0.2
459,492 - GFF2406 0.64 -0.2
459,492 - GFF2406 0.64 -0.2
459,537 - GFF2406 0.66 +0.4
459,591 - GFF2406 0.69 -0.9
459,729 - GFF2406 0.77 -0.3
459,797 + GFF2406 0.80 +0.3
459,797 + GFF2406 0.80 +0.2
459,798 - GFF2406 0.80 +1.8
459,798 - GFF2406 0.80 -0.7
460,182 - -1.1
460,333 + GFF2407 0.36 +0.3
460,333 + GFF2407 0.36 -0.1
460,333 + GFF2407 0.36 +0.3
460,333 + GFF2407 0.36 -0.7
460,333 + GFF2407 0.36 +1.2
460,333 + GFF2407 0.36 +0.2
460,333 + GFF2407 0.36 -2.0
460,333 + GFF2407 0.36 -0.0
460,333 + GFF2407 0.36 +0.3
460,334 - GFF2407 0.36 +1.8
460,334 - GFF2407 0.36 +0.2
460,334 - GFF2407 0.36 +0.6
460,334 - GFF2407 0.36 +1.4
460,334 - GFF2407 0.36 -0.4
460,468 + GFF2407 0.65 -0.5
460,468 + GFF2407 0.65 -0.7
460,469 - GFF2407 0.65 +0.4
460,505 - GFF2407 0.73 -0.8
461,042 + GFF2408 0.54 -0.6
461,043 - GFF2408 0.54 +0.7
461,043 - GFF2408 0.54 +0.6

Or see this region's nucleotide sequence