Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2285 and GFF2286 are separated by 117 nucleotides GFF2286 and GFF2287 overlap by 4 nucleotides GFF2287 and GFF2288 overlap by 4 nucleotides
GFF2285 - Transcriptional regulator, RpiR family, at 340,391 to 341,281
GFF2285
GFF2286 - BUG/TctC family periplasmic protein, at 341,399 to 342,394
GFF2286
GFF2287 - ortholog of Bordetella pertussis (BX470248) BP3300, at 342,391 to 343,353
GFF2287
GFF2288 - Glutathione-regulated potassium-efflux system protein KefC, at 343,350 to 345,206
GFF2288
Position (kb) 341
342
343 Strain fitness (log2 ratio) -1
0
1
2
3 at 340.532 kb on + strand, within GFF2285 at 340.551 kb on + strand, within GFF2285 at 340.551 kb on + strand, within GFF2285 at 340.552 kb on - strand, within GFF2285 at 340.671 kb on + strand, within GFF2285 at 340.671 kb on + strand, within GFF2285 at 340.671 kb on + strand, within GFF2285 at 340.672 kb on - strand, within GFF2285 at 341.094 kb on + strand, within GFF2285 at 341.094 kb on + strand, within GFF2285 at 341.095 kb on - strand, within GFF2285 at 341.095 kb on - strand, within GFF2285 at 341.253 kb on + strand at 341.253 kb on + strand at 341.387 kb on - strand at 341.567 kb on + strand, within GFF2286 at 341.567 kb on + strand, within GFF2286 at 341.567 kb on + strand, within GFF2286 at 341.567 kb on + strand, within GFF2286 at 341.568 kb on - strand, within GFF2286 at 341.678 kb on + strand, within GFF2286 at 341.679 kb on - strand, within GFF2286 at 341.679 kb on - strand, within GFF2286 at 341.744 kb on + strand, within GFF2286 at 341.745 kb on - strand, within GFF2286 at 341.882 kb on + strand, within GFF2286 at 341.882 kb on + strand, within GFF2286 at 341.883 kb on - strand, within GFF2286 at 341.883 kb on - strand, within GFF2286 at 341.883 kb on - strand, within GFF2286 at 341.883 kb on - strand, within GFF2286 at 342.469 kb on + strand at 342.469 kb on + strand at 342.469 kb on + strand at 342.470 kb on - strand at 342.470 kb on - strand at 342.470 kb on - strand at 342.470 kb on - strand at 342.470 kb on - strand at 342.496 kb on + strand, within GFF2287 at 342.496 kb on + strand, within GFF2287 at 342.496 kb on + strand, within GFF2287 at 342.497 kb on - strand, within GFF2287 at 342.661 kb on + strand, within GFF2287 at 342.661 kb on + strand, within GFF2287 at 342.661 kb on + strand, within GFF2287 at 342.662 kb on - strand, within GFF2287 at 342.662 kb on - strand, within GFF2287 at 342.662 kb on - strand, within GFF2287 at 342.662 kb on - strand, within GFF2287 at 342.662 kb on - strand, within GFF2287 at 342.662 kb on - strand, within GFF2287 at 342.688 kb on + strand, within GFF2287 at 342.688 kb on + strand, within GFF2287 at 342.689 kb on - strand, within GFF2287 at 342.814 kb on + strand, within GFF2287 at 342.814 kb on + strand, within GFF2287 at 342.815 kb on - strand, within GFF2287 at 342.815 kb on - strand, within GFF2287 at 342.815 kb on - strand, within GFF2287 at 342.815 kb on - strand, within GFF2287 at 342.815 kb on - strand, within GFF2287 at 342.815 kb on - strand, within GFF2287 at 342.866 kb on - strand, within GFF2287 at 342.866 kb on - strand, within GFF2287 at 342.866 kb on - strand, within GFF2287 at 342.866 kb on - strand, within GFF2287 at 342.971 kb on - strand, within GFF2287 at 343.333 kb on + strand at 343.333 kb on + strand at 343.334 kb on - strand at 343.351 kb on + strand at 343.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 340,532 + GFF2285 0.16 +0.5 340,551 + GFF2285 0.18 -0.6 340,551 + GFF2285 0.18 +1.1 340,552 - GFF2285 0.18 +0.1 340,671 + GFF2285 0.31 -1.3 340,671 + GFF2285 0.31 -0.1 340,671 + GFF2285 0.31 +0.8 340,672 - GFF2285 0.32 +0.6 341,094 + GFF2285 0.79 +0.1 341,094 + GFF2285 0.79 -1.0 341,095 - GFF2285 0.79 -0.2 341,095 - GFF2285 0.79 +0.2 341,253 + +0.0 341,253 + -0.6 341,387 - +0.0 341,567 + GFF2286 0.17 +0.0 341,567 + GFF2286 0.17 +0.9 341,567 + GFF2286 0.17 +0.2 341,567 + GFF2286 0.17 -0.0 341,568 - GFF2286 0.17 -0.1 341,678 + GFF2286 0.28 +0.0 341,679 - GFF2286 0.28 +0.2 341,679 - GFF2286 0.28 +0.4 341,744 + GFF2286 0.35 -0.2 341,745 - GFF2286 0.35 -0.3 341,882 + GFF2286 0.48 +0.1 341,882 + GFF2286 0.48 +0.1 341,883 - GFF2286 0.49 -0.3 341,883 - GFF2286 0.49 -0.8 341,883 - GFF2286 0.49 -0.4 341,883 - GFF2286 0.49 -0.9 342,469 + +0.1 342,469 + -0.6 342,469 + +0.1 342,470 - -0.0 342,470 - +0.2 342,470 - -0.3 342,470 - -0.7 342,470 - +0.9 342,496 + GFF2287 0.11 +0.2 342,496 + GFF2287 0.11 -0.4 342,496 + GFF2287 0.11 +0.8 342,497 - GFF2287 0.11 -1.2 342,661 + GFF2287 0.28 -0.0 342,661 + GFF2287 0.28 -0.2 342,661 + GFF2287 0.28 -0.3 342,662 - GFF2287 0.28 +2.8 342,662 - GFF2287 0.28 -0.6 342,662 - GFF2287 0.28 -0.6 342,662 - GFF2287 0.28 +0.3 342,662 - GFF2287 0.28 -0.5 342,662 - GFF2287 0.28 -0.5 342,688 + GFF2287 0.31 -0.4 342,688 + GFF2287 0.31 -0.5 342,689 - GFF2287 0.31 -1.2 342,814 + GFF2287 0.44 +0.6 342,814 + GFF2287 0.44 -1.2 342,815 - GFF2287 0.44 -1.0 342,815 - GFF2287 0.44 +0.9 342,815 - GFF2287 0.44 -0.7 342,815 - GFF2287 0.44 -0.4 342,815 - GFF2287 0.44 +0.4 342,815 - GFF2287 0.44 +0.5 342,866 - GFF2287 0.49 -0.4 342,866 - GFF2287 0.49 +0.4 342,866 - GFF2287 0.49 -0.8 342,866 - GFF2287 0.49 -0.3 342,971 - GFF2287 0.60 -1.0 343,333 + +0.8 343,333 + -0.4 343,334 - -0.5 343,351 + -0.8 343,352 - +0.1
Or see this region's nucleotide sequence