Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2252 and GFF2253 are separated by 4 nucleotides GFF2253 and GFF2254 are separated by 58 nucleotides GFF2254 and GFF2255 are separated by 8 nucleotides
GFF2252 - RNA-2',3'-PO4:RNA-5'-OH ligase, at 308,221 to 309,456
GFF2252
GFF2253 - no description, at 309,461 to 309,748
GFF2253
GFF2254 - HYPOTHETICAL/UNKNOWN PROTEIN, at 309,807 to 310,448
GFF2254
GFF2255 - Ribonuclease Z (EC 3.1.26.11), at 310,457 to 311,413
GFF2255
Position (kb) 309
310 Strain fitness (log2 ratio) -2
-1
0
1 at 308.936 kb on - strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.077 kb on - strand, within GFF2252 at 309.265 kb on - strand, within GFF2252 at 309.422 kb on - strand at 309.422 kb on - strand at 309.422 kb on - strand at 309.578 kb on + strand, within GFF2253 at 309.578 kb on + strand, within GFF2253 at 309.579 kb on - strand, within GFF2253 at 309.745 kb on - strand at 309.745 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.780 kb on + strand at 309.781 kb on - strand at 309.781 kb on - strand at 309.788 kb on + strand at 309.969 kb on + strand, within GFF2254 at 309.970 kb on - strand, within GFF2254 at 309.970 kb on - strand, within GFF2254 at 309.982 kb on - strand, within GFF2254 at 310.003 kb on - strand, within GFF2254 at 310.003 kb on - strand, within GFF2254 at 310.003 kb on - strand, within GFF2254 at 310.077 kb on + strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.097 kb on + strand, within GFF2254 at 310.098 kb on - strand, within GFF2254 at 310.387 kb on - strand at 310.664 kb on + strand, within GFF2255
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 308,936 - GFF2252 0.58 +0.3 309,076 + GFF2252 0.69 -0.1 309,076 + GFF2252 0.69 -1.3 309,076 + GFF2252 0.69 -0.6 309,076 + GFF2252 0.69 -0.2 309,077 - GFF2252 0.69 -0.8 309,265 - GFF2252 0.84 -0.2 309,422 - -0.2 309,422 - +0.5 309,422 - -0.3 309,578 + GFF2253 0.41 +0.0 309,578 + GFF2253 0.41 +0.4 309,579 - GFF2253 0.41 -0.1 309,745 - +0.5 309,745 - -1.7 309,747 - -0.7 309,747 - +0.4 309,747 - +1.5 309,747 - -0.3 309,747 - -2.1 309,747 - +0.8 309,780 + -1.2 309,781 - -0.8 309,781 - -0.2 309,788 + -0.0 309,969 + GFF2254 0.25 +0.1 309,970 - GFF2254 0.25 -0.8 309,970 - GFF2254 0.25 -0.5 309,982 - GFF2254 0.27 -1.0 310,003 - GFF2254 0.31 +0.0 310,003 - GFF2254 0.31 +0.3 310,003 - GFF2254 0.31 +0.5 310,077 + GFF2254 0.42 +0.5 310,078 - GFF2254 0.42 -0.5 310,078 - GFF2254 0.42 +0.4 310,078 - GFF2254 0.42 +1.1 310,078 - GFF2254 0.42 -0.0 310,097 + GFF2254 0.45 -0.2 310,098 - GFF2254 0.45 +0.8 310,387 - -1.0 310,664 + GFF2255 0.22 +0.4
Or see this region's nucleotide sequence