Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2150 and GFF2151 are separated by 84 nucleotides GFF2151 and GFF2152 overlap by 1 nucleotides GFF2152 and GFF2153 are separated by 19 nucleotides GFF2153 and GFF2154 are separated by 42 nucleotides
GFF2150 - Rubredoxin, at 199,066 to 199,218
GFF2150
GFF2151 - twitching motility protein PilG, at 199,303 to 199,701
GFF2151
GFF2152 - twitching motility protein PilH, at 199,701 to 200,066
GFF2152
GFF2153 - type IV pili signal transduction protein PilI, at 200,086 to 200,619
GFF2153
GFF2154 - twitching motility protein PilJ, at 200,662 to 202,968
GFF2154
Position (kb) 200
201 Strain fitness (log2 ratio) -2
-1
0
1
2 at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.324 kb on + strand at 199.324 kb on + strand at 199.324 kb on + strand at 199.325 kb on - strand at 199.325 kb on - strand at 199.501 kb on + strand, within GFF2151 at 199.501 kb on + strand, within GFF2151 at 199.502 kb on - strand, within GFF2151 at 199.502 kb on - strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.699 kb on + strand at 199.699 kb on + strand at 199.699 kb on + strand at 199.699 kb on + strand at 199.700 kb on - strand at 199.700 kb on - strand at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.931 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.203 kb on + strand, within GFF2153 at 200.203 kb on + strand, within GFF2153 at 200.203 kb on + strand, within GFF2153 at 200.203 kb on + strand, within GFF2153 at 200.204 kb on - strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.267 kb on - strand, within GFF2153 at 200.314 kb on + strand, within GFF2153 at 200.314 kb on + strand, within GFF2153 at 200.314 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.607 kb on + strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.617 kb on + strand at 200.617 kb on + strand at 200.617 kb on + strand at 200.617 kb on + strand at 200.618 kb on - strand at 200.618 kb on - strand at 200.693 kb on + strand at 200.693 kb on + strand at 200.694 kb on - strand at 201.229 kb on + strand, within GFF2154 at 201.229 kb on + strand, within GFF2154 at 201.230 kb on - strand, within GFF2154
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 199,229 + -0.5 199,229 + -0.9 199,229 + -1.8 199,229 + -0.9 199,229 + -0.8 199,229 + +0.5 199,229 + -1.5 199,229 + -0.1 199,229 + -0.1 199,229 + -0.0 199,229 + -0.5 199,229 + +0.0 199,229 + -0.4 199,229 + +0.2 199,229 + +0.2 199,229 + -0.3 199,229 + -0.9 199,229 + -0.5 199,229 + -1.0 199,229 + -0.3 199,230 - -0.7 199,230 - +1.3 199,230 - -0.3 199,230 - +0.1 199,230 - +0.6 199,230 - +0.1 199,230 - +0.9 199,230 - -0.5 199,230 - +0.7 199,230 - +0.6 199,230 - -0.6 199,230 - -0.4 199,230 - -0.1 199,230 - +0.5 199,233 + +0.0 199,233 + +0.1 199,233 + +0.2 199,233 + -0.2 199,233 + +0.8 199,233 + +0.5 199,233 + +0.3 199,233 + -1.3 199,233 + -1.2 199,233 + -0.2 199,233 + -1.3 199,233 + -1.3 199,233 + -0.2 199,233 + +0.1 199,233 + +0.4 199,233 + -0.5 199,233 + +0.3 199,233 + +0.2 199,233 + -1.6 199,233 + -0.1 199,234 - +0.8 199,234 - -0.2 199,234 - -0.0 199,234 - +0.0 199,234 - +0.1 199,234 - -0.1 199,234 - -0.7 199,234 - -1.2 199,234 - -2.3 199,234 - -0.5 199,234 - +0.5 199,234 - -0.2 199,234 - +0.5 199,234 - +0.1 199,234 - -2.0 199,234 - +0.8 199,234 - -1.2 199,234 - +0.1 199,234 - -0.4 199,234 - +0.5 199,234 - -1.6 199,234 - +1.0 199,234 - -1.2 199,234 - -1.1 199,234 - -0.2 199,239 + -0.8 199,239 + -0.8 199,239 + -1.8 199,239 + -0.4 199,239 + -1.4 199,240 - +0.4 199,240 - +0.5 199,240 - +0.2 199,240 - +1.0 199,240 - -0.3 199,240 - -1.3 199,324 + -1.0 199,324 + -0.5 199,324 + -1.9 199,325 - -0.9 199,325 - -0.1 199,501 + GFF2151 0.50 -2.2 199,501 + GFF2151 0.50 -1.1 199,502 - GFF2151 0.50 -1.0 199,502 - GFF2151 0.50 +0.1 199,580 + GFF2151 0.69 -1.9 199,580 + GFF2151 0.69 -0.6 199,580 + GFF2151 0.69 +1.1 199,580 + GFF2151 0.69 -2.0 199,580 + GFF2151 0.69 -1.0 199,580 + GFF2151 0.69 -1.0 199,581 - GFF2151 0.70 -0.3 199,581 - GFF2151 0.70 +0.4 199,581 - GFF2151 0.70 -0.9 199,581 - GFF2151 0.70 +0.5 199,581 - GFF2151 0.70 -1.8 199,581 - GFF2151 0.70 -1.1 199,581 - GFF2151 0.70 -0.8 199,581 - GFF2151 0.70 -0.9 199,581 - GFF2151 0.70 -1.2 199,581 - GFF2151 0.70 -1.4 199,621 + GFF2151 0.80 -2.5 199,621 + GFF2151 0.80 -1.6 199,621 + GFF2151 0.80 -1.3 199,621 + GFF2151 0.80 -1.0 199,621 + GFF2151 0.80 +0.0 199,621 + GFF2151 0.80 -1.6 199,622 - GFF2151 0.80 -1.1 199,622 - GFF2151 0.80 +0.3 199,622 - GFF2151 0.80 -0.1 199,622 - GFF2151 0.80 -1.4 199,622 - GFF2151 0.80 -0.5 199,622 - GFF2151 0.80 -0.9 199,622 - GFF2151 0.80 -1.0 199,699 + -0.6 199,699 + -0.6 199,699 + -0.8 199,699 + -0.9 199,700 - -0.3 199,700 - -0.6 199,923 + GFF2152 0.61 +0.3 199,923 + GFF2152 0.61 -0.0 199,923 + GFF2152 0.61 +2.3 199,923 + GFF2152 0.61 -0.3 199,923 + GFF2152 0.61 +0.5 199,923 + GFF2152 0.61 +1.1 199,923 + GFF2152 0.61 -1.2 199,923 + GFF2152 0.61 -1.0 199,924 - GFF2152 0.61 -0.2 199,924 - GFF2152 0.61 -1.1 199,924 - GFF2152 0.61 -0.9 199,924 - GFF2152 0.61 -1.1 199,924 - GFF2152 0.61 -1.2 199,924 - GFF2152 0.61 +0.2 199,924 - GFF2152 0.61 +0.9 199,931 + GFF2152 0.63 +0.2 200,007 + GFF2152 0.84 -0.4 200,007 + GFF2152 0.84 -1.4 200,007 + GFF2152 0.84 -0.3 200,007 + GFF2152 0.84 +0.1 200,007 + GFF2152 0.84 +0.5 200,008 - GFF2152 0.84 +1.2 200,008 - GFF2152 0.84 -0.8 200,008 - GFF2152 0.84 -0.4 200,008 - GFF2152 0.84 +1.1 200,008 - GFF2152 0.84 +0.7 200,008 - GFF2152 0.84 -0.8 200,008 - GFF2152 0.84 -1.5 200,203 + GFF2153 0.22 -0.9 200,203 + GFF2153 0.22 +0.4 200,203 + GFF2153 0.22 +0.3 200,203 + GFF2153 0.22 +0.5 200,204 - GFF2153 0.22 -0.6 200,266 + GFF2153 0.34 +0.1 200,266 + GFF2153 0.34 -0.4 200,266 + GFF2153 0.34 +1.6 200,266 + GFF2153 0.34 -1.5 200,267 - GFF2153 0.34 -0.1 200,314 + GFF2153 0.43 -0.5 200,314 + GFF2153 0.43 +0.0 200,314 + GFF2153 0.43 +0.0 200,533 + GFF2153 0.84 -0.3 200,533 + GFF2153 0.84 +1.0 200,533 + GFF2153 0.84 -1.4 200,533 + GFF2153 0.84 -0.0 200,533 + GFF2153 0.84 -0.8 200,533 + GFF2153 0.84 +0.4 200,533 + GFF2153 0.84 -1.3 200,534 - GFF2153 0.84 -0.7 200,534 - GFF2153 0.84 +1.0 200,534 - GFF2153 0.84 -1.1 200,534 - GFF2153 0.84 -0.7 200,534 - GFF2153 0.84 +0.8 200,534 - GFF2153 0.84 -0.5 200,534 - GFF2153 0.84 -0.2 200,534 - GFF2153 0.84 -0.2 200,534 - GFF2153 0.84 +0.1 200,607 + -0.6 200,608 - +0.3 200,608 - +0.7 200,608 - +2.0 200,608 - -1.5 200,608 - -1.0 200,617 + +0.8 200,617 + -1.5 200,617 + -0.1 200,617 + +1.6 200,618 - +0.3 200,618 - -0.3 200,693 + -0.2 200,693 + -0.2 200,694 - -1.2 201,229 + GFF2154 0.25 +0.2 201,229 + GFF2154 0.25 -2.2 201,230 - GFF2154 0.25 -1.1
Or see this region's nucleotide sequence