Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2020 and GFF2021 are separated by 127 nucleotides GFF2021 and GFF2022 overlap by 4 nucleotides
GFF2020 - BUG/TctC family periplasmic protein, at 63,553 to 64,575
GFF2020
GFF2021 - FIG00538369: hypothetical protein, at 64,703 to 65,926
GFF2021
GFF2022 - Acyl-CoA dehydrogenase, at 65,923 to 67,092
GFF2022
Position (kb) 64
65
66 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 63.865 kb on + strand, within GFF2020 at 63.865 kb on + strand, within GFF2020 at 63.866 kb on - strand, within GFF2020 at 63.866 kb on - strand, within GFF2020 at 63.866 kb on - strand, within GFF2020 at 63.866 kb on - strand, within GFF2020 at 63.931 kb on + strand, within GFF2020 at 63.932 kb on - strand, within GFF2020 at 63.932 kb on - strand, within GFF2020 at 64.069 kb on + strand, within GFF2020 at 64.069 kb on + strand, within GFF2020 at 64.069 kb on + strand, within GFF2020 at 64.069 kb on + strand, within GFF2020 at 64.070 kb on - strand, within GFF2020 at 64.070 kb on - strand, within GFF2020 at 64.070 kb on - strand, within GFF2020 at 64.070 kb on - strand, within GFF2020 at 64.070 kb on - strand, within GFF2020 at 64.150 kb on + strand, within GFF2020 at 64.150 kb on + strand, within GFF2020 at 64.150 kb on + strand, within GFF2020 at 64.150 kb on + strand, within GFF2020 at 64.150 kb on + strand, within GFF2020 at 64.151 kb on - strand, within GFF2020 at 64.151 kb on - strand, within GFF2020 at 64.633 kb on + strand at 64.701 kb on + strand at 64.708 kb on + strand at 64.708 kb on + strand at 64.708 kb on + strand at 64.708 kb on + strand at 64.708 kb on + strand at 64.708 kb on + strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.709 kb on - strand at 64.769 kb on + strand at 64.871 kb on - strand, within GFF2021 at 64.988 kb on + strand, within GFF2021 at 64.989 kb on - strand, within GFF2021 at 64.989 kb on - strand, within GFF2021 at 65.031 kb on - strand, within GFF2021 at 65.066 kb on + strand, within GFF2021 at 65.066 kb on + strand, within GFF2021 at 65.067 kb on - strand, within GFF2021 at 65.085 kb on - strand, within GFF2021 at 65.102 kb on + strand, within GFF2021 at 65.102 kb on + strand, within GFF2021 at 65.420 kb on + strand, within GFF2021 at 65.421 kb on - strand, within GFF2021 at 65.456 kb on + strand, within GFF2021 at 65.457 kb on - strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.708 kb on + strand, within GFF2021 at 66.001 kb on + strand at 66.002 kb on - strand at 66.493 kb on + strand, within GFF2022 at 66.493 kb on + strand, within GFF2022 at 66.494 kb on - strand, within GFF2022 at 66.494 kb on - strand, within GFF2022 at 66.494 kb on - strand, within GFF2022 at 66.637 kb on + strand, within GFF2022 at 66.637 kb on + strand, within GFF2022 at 66.637 kb on + strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.727 kb on + strand, within GFF2022 at 66.728 kb on - strand, within GFF2022 at 66.728 kb on - strand, within GFF2022 at 66.728 kb on - strand, within GFF2022 at 66.853 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.899 kb on - strand, within GFF2022 at 66.899 kb on - strand, within GFF2022
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 63,865 + GFF2020 0.30 -0.0 63,865 + GFF2020 0.30 -1.1 63,866 - GFF2020 0.31 -0.3 63,866 - GFF2020 0.31 -0.6 63,866 - GFF2020 0.31 -0.7 63,866 - GFF2020 0.31 +1.2 63,931 + GFF2020 0.37 -0.4 63,932 - GFF2020 0.37 +0.0 63,932 - GFF2020 0.37 -0.6 64,069 + GFF2020 0.50 -0.2 64,069 + GFF2020 0.50 -0.2 64,069 + GFF2020 0.50 +0.4 64,069 + GFF2020 0.50 -0.5 64,070 - GFF2020 0.51 -0.3 64,070 - GFF2020 0.51 +1.2 64,070 - GFF2020 0.51 -0.6 64,070 - GFF2020 0.51 -2.3 64,070 - GFF2020 0.51 -1.0 64,150 + GFF2020 0.58 -1.2 64,150 + GFF2020 0.58 -0.3 64,150 + GFF2020 0.58 +0.2 64,150 + GFF2020 0.58 +0.2 64,150 + GFF2020 0.58 -0.3 64,151 - GFF2020 0.58 -0.8 64,151 - GFF2020 0.58 +0.0 64,633 + +0.2 64,701 + -0.9 64,708 + -0.3 64,708 + -1.6 64,708 + -1.3 64,708 + -0.0 64,708 + +0.5 64,708 + -0.4 64,709 - -1.2 64,709 - -0.7 64,709 - -1.0 64,709 - +0.2 64,709 - -0.2 64,709 - -0.3 64,709 - +1.1 64,709 - +0.5 64,709 - -0.8 64,709 - +0.4 64,709 - -0.1 64,709 - -0.8 64,769 + +1.6 64,871 - GFF2021 0.14 -0.4 64,988 + GFF2021 0.23 -0.3 64,989 - GFF2021 0.23 -0.3 64,989 - GFF2021 0.23 +0.0 65,031 - GFF2021 0.27 -0.3 65,066 + GFF2021 0.30 -0.4 65,066 + GFF2021 0.30 -1.4 65,067 - GFF2021 0.30 -0.5 65,085 - GFF2021 0.31 -0.6 65,102 + GFF2021 0.33 -0.5 65,102 + GFF2021 0.33 +0.5 65,420 + GFF2021 0.59 -0.5 65,421 - GFF2021 0.59 +0.2 65,456 + GFF2021 0.62 -0.6 65,457 - GFF2021 0.62 -1.0 65,567 + GFF2021 0.71 -0.2 65,567 + GFF2021 0.71 -2.8 65,567 + GFF2021 0.71 +0.6 65,567 + GFF2021 0.71 -0.9 65,568 - GFF2021 0.71 -0.4 65,568 - GFF2021 0.71 -1.5 65,568 - GFF2021 0.71 -0.7 65,568 - GFF2021 0.71 -0.7 65,708 + GFF2021 0.82 -1.5 66,001 + -0.8 66,002 - -0.5 66,493 + GFF2022 0.49 -1.5 66,493 + GFF2022 0.49 -3.1 66,494 - GFF2022 0.49 +0.3 66,494 - GFF2022 0.49 -0.1 66,494 - GFF2022 0.49 -1.4 66,637 + GFF2022 0.61 +0.6 66,637 + GFF2022 0.61 -0.5 66,637 + GFF2022 0.61 +1.2 66,638 - GFF2022 0.61 +0.8 66,638 - GFF2022 0.61 -0.9 66,638 - GFF2022 0.61 +0.0 66,638 - GFF2022 0.61 -0.8 66,727 + GFF2022 0.69 +0.0 66,728 - GFF2022 0.69 +2.8 66,728 - GFF2022 0.69 -1.2 66,728 - GFF2022 0.69 +0.2 66,853 + GFF2022 0.79 +0.8 66,898 + GFF2022 0.83 -3.4 66,898 + GFF2022 0.83 -0.2 66,898 + GFF2022 0.83 +0.8 66,898 + GFF2022 0.83 -0.5 66,898 + GFF2022 0.83 -0.7 66,899 - GFF2022 0.83 +1.3 66,899 - GFF2022 0.83 -0.1
Or see this region's nucleotide sequence