Strain Fitness in Variovorax sp. SCN45 around GFF198

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF196 and GFF197 are separated by 26 nucleotidesGFF197 and GFF198 overlap by 4 nucleotidesGFF198 and GFF199 are separated by 14 nucleotidesGFF199 and GFF200 overlap by 1 nucleotides GFF196 - Probable ferredoxin subunit of a ring-hydroxylating dioxygenase oxidoreductase protein (EC 1.-.-.-), at 225,297 to 225,617 GFF196 GFF197 - BUG/TctC family periplasmic protein, at 225,644 to 226,612 GFF197 GFF198 - Isochorismate pyruvate-lyase (EC 4.2.99.21), at 226,609 to 226,977 GFF198 GFF199 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 226,992 to 227,471 GFF199 GFF200 - Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB, at 227,471 to 228,727 GFF200 Position (kb) 226 227Strain fitness (log2 ratio) -2 -1 0 1 2 3at 225.753 kb on + strand, within GFF197at 225.754 kb on - strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.069 kb on - strand, within GFF197at 226.309 kb on - strand, within GFF197at 226.309 kb on - strand, within GFF197at 226.323 kb on + strand, within GFF197at 226.324 kb on - strand, within GFF197at 226.324 kb on - strand, within GFF197at 226.324 kb on - strand, within GFF197at 226.359 kb on + strand, within GFF197at 226.359 kb on + strand, within GFF197at 226.359 kb on + strand, within GFF197at 226.359 kb on + strand, within GFF197at 226.359 kb on + strand, within GFF197at 226.359 kb on + strand, within GFF197at 226.360 kb on - strand, within GFF197at 226.360 kb on - strand, within GFF197at 226.360 kb on - strand, within GFF197at 226.713 kb on - strand, within GFF198at 226.916 kb on + strand, within GFF198at 226.916 kb on + strand, within GFF198at 226.916 kb on + strand, within GFF198at 226.916 kb on + strand, within GFF198at 226.916 kb on + strand, within GFF198at 226.917 kb on - strand, within GFF198at 226.917 kb on - strand, within GFF198at 227.095 kb on + strand, within GFF199at 227.095 kb on + strand, within GFF199at 227.096 kb on - strand, within GFF199at 227.152 kb on + strand, within GFF199at 227.152 kb on + strand, within GFF199at 227.153 kb on - strand, within GFF199at 227.153 kb on - strand, within GFF199at 227.228 kb on - strand, within GFF199at 227.228 kb on - strand, within GFF199at 227.228 kb on - strand, within GFF199at 227.228 kb on - strand, within GFF199at 227.230 kb on + strand, within GFF199at 227.231 kb on - strand, within GFF199at 227.231 kb on - strand, within GFF199at 227.263 kb on + strand, within GFF199at 227.353 kb on + strand, within GFF199at 227.353 kb on + strand, within GFF199at 227.354 kb on - strand, within GFF199at 227.419 kb on + strand, within GFF199at 227.429 kb on - strandat 227.429 kb on - strandat 227.449 kb on + strandat 227.449 kb on + strandat 227.450 kb on - strandat 227.450 kb on - strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.502 kb on + strandat 227.503 kb on - strandat 227.503 kb on - strandat 227.503 kb on - strandat 227.503 kb on - strandat 227.503 kb on - strandat 227.586 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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225,753 + GFF197 0.11 +0.7
225,754 - GFF197 0.11 -0.2
225,858 + GFF197 0.22 +0.7
225,858 + GFF197 0.22 +0.4
225,858 + GFF197 0.22 -1.3
225,858 + GFF197 0.22 -0.1
225,858 + GFF197 0.22 -2.3
225,859 - GFF197 0.22 -0.3
225,859 - GFF197 0.22 -2.3
225,859 - GFF197 0.22 -0.4
225,859 - GFF197 0.22 -0.4
225,859 - GFF197 0.22 -0.3
225,859 - GFF197 0.22 -0.0
226,068 + GFF197 0.44 -0.3
226,068 + GFF197 0.44 +0.7
226,068 + GFF197 0.44 +0.7
226,068 + GFF197 0.44 -0.1
226,069 - GFF197 0.44 +0.7
226,309 - GFF197 0.69 -0.3
226,309 - GFF197 0.69 +0.5
226,323 + GFF197 0.70 +0.3
226,324 - GFF197 0.70 -1.5
226,324 - GFF197 0.70 -1.0
226,324 - GFF197 0.70 -0.5
226,359 + GFF197 0.74 -0.0
226,359 + GFF197 0.74 -0.1
226,359 + GFF197 0.74 +3.3
226,359 + GFF197 0.74 +1.1
226,359 + GFF197 0.74 -1.1
226,359 + GFF197 0.74 +1.9
226,360 - GFF197 0.74 +0.6
226,360 - GFF197 0.74 -0.7
226,360 - GFF197 0.74 -0.6
226,713 - GFF198 0.28 +1.5
226,916 + GFF198 0.83 -0.7
226,916 + GFF198 0.83 +0.6
226,916 + GFF198 0.83 -0.7
226,916 + GFF198 0.83 -1.1
226,916 + GFF198 0.83 +0.8
226,917 - GFF198 0.83 +0.7
226,917 - GFF198 0.83 -0.7
227,095 + GFF199 0.21 -0.4
227,095 + GFF199 0.21 -0.3
227,096 - GFF199 0.22 -0.3
227,152 + GFF199 0.33 +0.4
227,152 + GFF199 0.33 +0.6
227,153 - GFF199 0.34 -0.4
227,153 - GFF199 0.34 -1.3
227,228 - GFF199 0.49 -1.0
227,228 - GFF199 0.49 -0.1
227,228 - GFF199 0.49 -1.7
227,228 - GFF199 0.49 -0.2
227,230 + GFF199 0.50 -0.2
227,231 - GFF199 0.50 +0.3
227,231 - GFF199 0.50 +0.7
227,263 + GFF199 0.56 +1.0
227,353 + GFF199 0.75 -1.0
227,353 + GFF199 0.75 -0.7
227,354 - GFF199 0.75 +0.2
227,419 + GFF199 0.89 -0.4
227,429 - -1.0
227,429 - -0.9
227,449 + -0.4
227,449 + +1.3
227,450 - -0.0
227,450 - -0.1
227,502 + +2.3
227,502 + +0.4
227,502 + -0.7
227,502 + -0.5
227,502 + +0.3
227,502 + +0.7
227,502 + +1.7
227,502 + -0.7
227,502 + -1.0
227,502 + +0.4
227,502 + +0.0
227,502 + -0.2
227,502 + +0.8
227,503 - -1.0
227,503 - -0.2
227,503 - -0.4
227,503 - +1.5
227,503 - -0.6
227,586 + +0.4

Or see this region's nucleotide sequence