Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1940 and GFF1941 are separated by 8 nucleotides GFF1941 and GFF1942 are separated by 71 nucleotides GFF1942 and GFF1943 are separated by 51 nucleotides GFF1943 and GFF1944 are separated by 113 nucleotides
GFF1940 - Adenylosuccinate synthetase-like protein, at 633,764 to 635,107
GFF1940
GFF1941 - hypothetical protein, at 635,116 to 635,544
GFF1941
GFF1942 - no description, at 635,616 to 635,741
GFF1942
GFF1943 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 635,793 to 638,579
GFF1943
GFF1944 - hypothetical protein, at 638,693 to 639,622
GFF1944
Position (kb) 635
636
637
638
639 Strain fitness (log2 ratio) -2
-1
0
1
2 at 634.976 kb on + strand at 634.977 kb on - strand at 634.977 kb on - strand at 635.125 kb on + strand at 635.125 kb on + strand at 635.398 kb on + strand, within GFF1941 at 635.399 kb on - strand, within GFF1941 at 635.613 kb on + strand at 635.614 kb on - strand at 635.617 kb on + strand at 635.618 kb on - strand at 635.618 kb on - strand at 635.618 kb on - strand at 635.814 kb on + strand at 635.814 kb on + strand at 635.814 kb on + strand at 635.829 kb on - strand at 636.088 kb on - strand, within GFF1943 at 636.123 kb on + strand, within GFF1943 at 636.123 kb on + strand, within GFF1943 at 636.124 kb on - strand, within GFF1943 at 636.570 kb on + strand, within GFF1943 at 636.570 kb on + strand, within GFF1943 at 636.571 kb on - strand, within GFF1943 at 636.571 kb on - strand, within GFF1943 at 636.793 kb on - strand, within GFF1943 at 636.890 kb on + strand, within GFF1943 at 636.914 kb on + strand, within GFF1943 at 636.991 kb on - strand, within GFF1943 at 637.060 kb on - strand, within GFF1943 at 637.309 kb on - strand, within GFF1943 at 637.315 kb on - strand, within GFF1943 at 637.315 kb on - strand, within GFF1943 at 637.485 kb on - strand, within GFF1943 at 637.580 kb on + strand, within GFF1943 at 637.644 kb on + strand, within GFF1943 at 637.644 kb on + strand, within GFF1943 at 637.665 kb on + strand, within GFF1943 at 637.665 kb on + strand, within GFF1943 at 637.666 kb on - strand, within GFF1943 at 637.666 kb on - strand, within GFF1943 at 637.683 kb on + strand, within GFF1943 at 637.683 kb on + strand, within GFF1943 at 637.822 kb on - strand, within GFF1943 at 637.967 kb on + strand, within GFF1943 at 638.049 kb on + strand, within GFF1943 at 638.172 kb on + strand, within GFF1943 at 638.190 kb on + strand, within GFF1943 at 638.247 kb on + strand, within GFF1943 at 638.247 kb on + strand, within GFF1943 at 638.247 kb on + strand, within GFF1943 at 638.323 kb on - strand at 638.331 kb on + strand at 638.430 kb on + strand at 638.430 kb on + strand at 638.430 kb on + strand at 638.431 kb on - strand at 638.493 kb on + strand at 638.494 kb on - strand at 638.494 kb on - strand at 638.646 kb on + strand at 638.711 kb on + strand at 638.712 kb on - strand at 638.715 kb on + strand at 639.119 kb on + strand, within GFF1944 at 639.119 kb on + strand, within GFF1944 at 639.119 kb on + strand, within GFF1944 at 639.119 kb on + strand, within GFF1944 at 639.120 kb on - strand, within GFF1944 at 639.180 kb on - strand, within GFF1944 at 639.524 kb on + strand, within GFF1944 at 639.524 kb on + strand, within GFF1944 at 639.525 kb on - strand, within GFF1944 at 639.525 kb on - strand, within GFF1944 at 639.525 kb on - strand, within GFF1944 at 639.539 kb on + strand at 639.548 kb on + strand at 639.548 kb on + strand at 639.554 kb on + strand at 639.554 kb on + strand at 639.554 kb on + strand at 639.554 kb on + strand at 639.554 kb on + strand at 639.555 kb on - strand at 639.576 kb on + strand at 639.576 kb on + strand at 639.577 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 634,976 + -0.2 634,977 - -0.2 634,977 - -0.8 635,125 + -0.7 635,125 + +0.3 635,398 + GFF1941 0.66 -2.1 635,399 - GFF1941 0.66 -0.4 635,613 + -0.7 635,614 - -0.0 635,617 + -1.3 635,618 - -0.2 635,618 - -0.0 635,618 - -1.7 635,814 + +0.9 635,814 + +0.1 635,814 + +0.3 635,829 - -0.1 636,088 - GFF1943 0.11 +0.0 636,123 + GFF1943 0.12 -0.3 636,123 + GFF1943 0.12 +0.4 636,124 - GFF1943 0.12 -1.8 636,570 + GFF1943 0.28 -1.0 636,570 + GFF1943 0.28 -1.8 636,571 - GFF1943 0.28 -0.1 636,571 - GFF1943 0.28 -0.2 636,793 - GFF1943 0.36 -0.3 636,890 + GFF1943 0.39 +0.4 636,914 + GFF1943 0.40 -0.1 636,991 - GFF1943 0.43 +0.0 637,060 - GFF1943 0.45 +0.1 637,309 - GFF1943 0.54 -1.9 637,315 - GFF1943 0.55 +0.9 637,315 - GFF1943 0.55 +0.3 637,485 - GFF1943 0.61 +0.2 637,580 + GFF1943 0.64 -0.7 637,644 + GFF1943 0.66 +1.2 637,644 + GFF1943 0.66 +0.1 637,665 + GFF1943 0.67 -0.9 637,665 + GFF1943 0.67 +1.8 637,666 - GFF1943 0.67 -0.2 637,666 - GFF1943 0.67 +0.1 637,683 + GFF1943 0.68 +0.4 637,683 + GFF1943 0.68 +0.6 637,822 - GFF1943 0.73 -0.5 637,967 + GFF1943 0.78 -0.8 638,049 + GFF1943 0.81 +0.0 638,172 + GFF1943 0.85 +0.0 638,190 + GFF1943 0.86 +0.2 638,247 + GFF1943 0.88 +0.1 638,247 + GFF1943 0.88 +2.4 638,247 + GFF1943 0.88 -0.4 638,323 - +0.1 638,331 + -0.7 638,430 + +0.8 638,430 + +0.6 638,430 + -0.8 638,431 - +0.0 638,493 + -1.3 638,494 - -0.2 638,494 - -1.0 638,646 + +0.0 638,711 + +1.0 638,712 - -0.9 638,715 + -0.0 639,119 + GFF1944 0.46 +0.5 639,119 + GFF1944 0.46 -0.2 639,119 + GFF1944 0.46 +0.1 639,119 + GFF1944 0.46 -0.5 639,120 - GFF1944 0.46 -0.2 639,180 - GFF1944 0.52 -0.1 639,524 + GFF1944 0.89 -1.3 639,524 + GFF1944 0.89 -0.7 639,525 - GFF1944 0.89 -0.1 639,525 - GFF1944 0.89 -0.5 639,525 - GFF1944 0.89 -0.6 639,539 + +0.0 639,548 + -1.1 639,548 + +1.4 639,554 + -0.1 639,554 + -1.2 639,554 + +0.0 639,554 + -0.5 639,554 + -0.1 639,555 - +0.1 639,576 + +0.6 639,576 + +0.2 639,577 - -1.5
Or see this region's nucleotide sequence