Strain Fitness in Variovorax sp. SCN45 around GFF1812

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1811 and GFF1812 overlap by 4 nucleotidesGFF1812 and GFF1813 are separated by 36 nucleotides GFF1811 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 474,752 to 476,101 GFF1811 GFF1812 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 476,098 to 476,751 GFF1812 GFF1813 - Acetylornithine deacetylase-like protein Acry_1162, at 476,788 to 478,014 GFF1813 Position (kb) 476 477Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 475.148 kb on + strand, within GFF1811at 475.149 kb on - strand, within GFF1811at 475.172 kb on + strand, within GFF1811at 475.172 kb on + strand, within GFF1811at 475.172 kb on + strand, within GFF1811at 475.172 kb on + strand, within GFF1811at 475.172 kb on + strand, within GFF1811at 475.173 kb on - strand, within GFF1811at 475.173 kb on - strand, within GFF1811at 475.173 kb on - strand, within GFF1811at 475.214 kb on + strand, within GFF1811at 475.214 kb on + strand, within GFF1811at 475.215 kb on - strand, within GFF1811at 475.215 kb on - strand, within GFF1811at 475.215 kb on - strand, within GFF1811at 475.289 kb on + strand, within GFF1811at 475.289 kb on + strand, within GFF1811at 475.289 kb on + strand, within GFF1811at 475.290 kb on - strand, within GFF1811at 475.290 kb on - strand, within GFF1811at 475.290 kb on - strand, within GFF1811at 475.290 kb on - strand, within GFF1811at 475.337 kb on + strand, within GFF1811at 475.338 kb on - strand, within GFF1811at 475.338 kb on - strand, within GFF1811at 475.745 kb on + strand, within GFF1811at 475.745 kb on + strand, within GFF1811at 475.745 kb on + strand, within GFF1811at 475.746 kb on - strand, within GFF1811at 475.746 kb on - strand, within GFF1811at 475.826 kb on + strand, within GFF1811at 475.917 kb on - strand, within GFF1811at 475.917 kb on - strand, within GFF1811at 475.917 kb on - strand, within GFF1811at 475.917 kb on - strand, within GFF1811at 476.048 kb on + strandat 476.048 kb on + strandat 476.049 kb on - strandat 476.429 kb on - strand, within GFF1812at 476.429 kb on - strand, within GFF1812at 476.429 kb on - strand, within GFF1812at 476.429 kb on - strand, within GFF1812at 476.458 kb on + strand, within GFF1812at 476.458 kb on + strand, within GFF1812at 476.458 kb on + strand, within GFF1812at 476.459 kb on - strand, within GFF1812at 476.648 kb on - strand, within GFF1812at 476.852 kb on - strandat 476.912 kb on + strand, within GFF1813at 476.912 kb on + strand, within GFF1813at 476.913 kb on - strand, within GFF1813at 476.942 kb on + strand, within GFF1813at 476.942 kb on + strand, within GFF1813at 476.943 kb on - strand, within GFF1813at 476.943 kb on - strand, within GFF1813at 476.943 kb on - strand, within GFF1813at 476.960 kb on + strand, within GFF1813at 476.960 kb on + strand, within GFF1813at 476.960 kb on + strand, within GFF1813at 476.960 kb on + strand, within GFF1813at 477.302 kb on + strand, within GFF1813at 477.302 kb on + strand, within GFF1813at 477.303 kb on - strand, within GFF1813at 477.320 kb on + strand, within GFF1813at 477.320 kb on + strand, within GFF1813at 477.320 kb on + strand, within GFF1813at 477.321 kb on - strand, within GFF1813at 477.321 kb on - strand, within GFF1813at 477.321 kb on - strand, within GFF1813at 477.452 kb on + strand, within GFF1813at 477.452 kb on + strand, within GFF1813at 477.453 kb on - strand, within GFF1813at 477.453 kb on - strand, within GFF1813at 477.545 kb on + strand, within GFF1813at 477.545 kb on + strand, within GFF1813at 477.545 kb on + strand, within GFF1813at 477.690 kb on - strand, within GFF1813

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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475,148 + GFF1811 0.29 -0.3
475,149 - GFF1811 0.29 +0.3
475,172 + GFF1811 0.31 +0.4
475,172 + GFF1811 0.31 +1.0
475,172 + GFF1811 0.31 -1.4
475,172 + GFF1811 0.31 -0.5
475,172 + GFF1811 0.31 -0.3
475,173 - GFF1811 0.31 -2.5
475,173 - GFF1811 0.31 -0.3
475,173 - GFF1811 0.31 +0.2
475,214 + GFF1811 0.34 -0.6
475,214 + GFF1811 0.34 -0.0
475,215 - GFF1811 0.34 -1.7
475,215 - GFF1811 0.34 -0.6
475,215 - GFF1811 0.34 -1.0
475,289 + GFF1811 0.40 +0.0
475,289 + GFF1811 0.40 +0.2
475,289 + GFF1811 0.40 +2.8
475,290 - GFF1811 0.40 -0.3
475,290 - GFF1811 0.40 -1.6
475,290 - GFF1811 0.40 -1.7
475,290 - GFF1811 0.40 -1.1
475,337 + GFF1811 0.43 -0.5
475,338 - GFF1811 0.43 +0.3
475,338 - GFF1811 0.43 -0.4
475,745 + GFF1811 0.74 -0.9
475,745 + GFF1811 0.74 -0.3
475,745 + GFF1811 0.74 -0.6
475,746 - GFF1811 0.74 -0.3
475,746 - GFF1811 0.74 +0.2
475,826 + GFF1811 0.80 +0.4
475,917 - GFF1811 0.86 +0.4
475,917 - GFF1811 0.86 -0.1
475,917 - GFF1811 0.86 -0.6
475,917 - GFF1811 0.86 +0.2
476,048 + -0.1
476,048 + +0.4
476,049 - -1.1
476,429 - GFF1812 0.51 +0.5
476,429 - GFF1812 0.51 +0.1
476,429 - GFF1812 0.51 +0.3
476,429 - GFF1812 0.51 +0.8
476,458 + GFF1812 0.55 +0.9
476,458 + GFF1812 0.55 -0.7
476,458 + GFF1812 0.55 -0.2
476,459 - GFF1812 0.55 -0.6
476,648 - GFF1812 0.84 +1.8
476,852 - -0.8
476,912 + GFF1813 0.10 -1.1
476,912 + GFF1813 0.10 -0.6
476,913 - GFF1813 0.10 -0.1
476,942 + GFF1813 0.13 -1.4
476,942 + GFF1813 0.13 -0.8
476,943 - GFF1813 0.13 -0.7
476,943 - GFF1813 0.13 -1.2
476,943 - GFF1813 0.13 +1.4
476,960 + GFF1813 0.14 +0.3
476,960 + GFF1813 0.14 -0.8
476,960 + GFF1813 0.14 -0.8
476,960 + GFF1813 0.14 +0.4
477,302 + GFF1813 0.42 -0.8
477,302 + GFF1813 0.42 +0.7
477,303 - GFF1813 0.42 -0.5
477,320 + GFF1813 0.43 +0.4
477,320 + GFF1813 0.43 +0.6
477,320 + GFF1813 0.43 -0.4
477,321 - GFF1813 0.43 -0.4
477,321 - GFF1813 0.43 +0.4
477,321 - GFF1813 0.43 +0.8
477,452 + GFF1813 0.54 -0.6
477,452 + GFF1813 0.54 -0.6
477,453 - GFF1813 0.54 -0.2
477,453 - GFF1813 0.54 +2.2
477,545 + GFF1813 0.62 -2.8
477,545 + GFF1813 0.62 -0.4
477,545 + GFF1813 0.62 +0.2
477,690 - GFF1813 0.74 -0.0

Or see this region's nucleotide sequence