Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1673 and GFF1674 overlap by 4 nucleotides GFF1674 and GFF1675 overlap by 8 nucleotides GFF1675 and GFF1676 overlap by 4 nucleotides GFF1676 and GFF1677 are separated by 36 nucleotides
GFF1673 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 318,856 to 319,566
GFF1673
GFF1674 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 319,563 to 320,324
GFF1674
GFF1675 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 320,317 to 321,297
GFF1675
GFF1676 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 321,294 to 322,166
GFF1676
GFF1677 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 322,203 to 323,405
GFF1677
Position (kb) 320
321
322 Strain fitness (log2 ratio) -2
-1
0
1
2 at 319.621 kb on + strand at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.597 kb on - strand, within GFF1675 at 320.601 kb on - strand, within GFF1675 at 320.601 kb on - strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.619 kb on - strand, within GFF1675 at 320.619 kb on - strand, within GFF1675 at 321.089 kb on + strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.182 kb on + strand, within GFF1675 at 321.183 kb on - strand, within GFF1675 at 321.183 kb on - strand, within GFF1675 at 321.523 kb on + strand, within GFF1676 at 321.523 kb on + strand, within GFF1676 at 321.524 kb on - strand, within GFF1676 at 321.526 kb on + strand, within GFF1676 at 321.526 kb on + strand, within GFF1676 at 321.592 kb on + strand, within GFF1676 at 321.928 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.974 kb on - strand, within GFF1676 at 322.007 kb on - strand, within GFF1676 at 322.072 kb on + strand, within GFF1676 at 322.072 kb on + strand, within GFF1676 at 322.073 kb on - strand, within GFF1676 at 322.096 kb on - strand at 322.105 kb on + strand at 322.105 kb on + strand at 322.161 kb on + strand at 322.161 kb on + strand at 322.161 kb on + strand at 322.161 kb on + strand at 322.162 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 319,621 + -0.8 320,423 + GFF1675 0.11 -1.9 320,423 + GFF1675 0.11 -1.5 320,423 + GFF1675 0.11 -1.7 320,423 + GFF1675 0.11 -0.5 320,423 + GFF1675 0.11 +0.2 320,597 - GFF1675 0.29 -0.6 320,601 - GFF1675 0.29 +0.3 320,601 - GFF1675 0.29 +0.0 320,618 + GFF1675 0.31 +0.1 320,618 + GFF1675 0.31 -0.4 320,618 + GFF1675 0.31 -0.8 320,619 - GFF1675 0.31 -0.8 320,619 - GFF1675 0.31 -0.5 321,089 + GFF1675 0.79 +0.0 321,090 - GFF1675 0.79 -0.2 321,090 - GFF1675 0.79 -0.2 321,090 - GFF1675 0.79 -0.2 321,182 + GFF1675 0.88 -0.3 321,183 - GFF1675 0.88 +0.2 321,183 - GFF1675 0.88 +0.7 321,523 + GFF1676 0.26 -0.0 321,523 + GFF1676 0.26 -0.2 321,524 - GFF1676 0.26 +0.2 321,526 + GFF1676 0.27 -0.1 321,526 + GFF1676 0.27 -0.8 321,592 + GFF1676 0.34 -0.2 321,928 + GFF1676 0.73 -0.5 321,973 + GFF1676 0.78 +1.3 321,973 + GFF1676 0.78 -0.3 321,973 + GFF1676 0.78 +1.0 321,973 + GFF1676 0.78 -0.2 321,973 + GFF1676 0.78 +0.2 321,974 - GFF1676 0.78 -0.8 322,007 - GFF1676 0.82 +0.1 322,072 + GFF1676 0.89 +1.8 322,072 + GFF1676 0.89 +0.4 322,073 - GFF1676 0.89 +1.2 322,096 - -0.3 322,105 + +0.1 322,105 + -1.0 322,161 + +0.3 322,161 + -0.5 322,161 + +0.3 322,161 + +0.3 322,162 - -0.3
Or see this region's nucleotide sequence