Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF155 and GFF156 are separated by 16 nucleotides GFF156 and GFF157 overlap by 4 nucleotides GFF157 and GFF158 overlap by 4 nucleotides GFF158 and GFF159 overlap by 13 nucleotides
GFF155 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 183,212 to 184,783
GFF155
GFF156 - Glycosyl transferase, group 2 family, at 184,800 to 185,498
GFF156
GFF157 - Methyltransferase type 12, at 185,495 to 186,100
GFF157
GFF158 - LmbE-like protein, at 186,097 to 186,858
GFF158
GFF159 - Acyl-CoA dehydrogenase/oxidase domain protein, at 186,846 to 187,865
GFF159
Position (kb) 185
186
187 Strain fitness (log2 ratio) -2
-1
0
1 at 184.499 kb on + strand, within GFF155 at 184.500 kb on - strand, within GFF155 at 184.500 kb on - strand, within GFF155 at 184.657 kb on + strand at 184.657 kb on + strand at 184.657 kb on + strand at 184.657 kb on + strand at 184.657 kb on + strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.907 kb on + strand, within GFF156 at 184.908 kb on - strand, within GFF156 at 184.908 kb on - strand, within GFF156 at 185.083 kb on + strand, within GFF156 at 185.083 kb on + strand, within GFF156 at 185.084 kb on - strand, within GFF156 at 185.084 kb on - strand, within GFF156 at 185.746 kb on - strand, within GFF157 at 185.958 kb on + strand, within GFF157 at 185.958 kb on + strand, within GFF157 at 185.959 kb on - strand, within GFF157 at 185.971 kb on - strand, within GFF157 at 185.971 kb on - strand, within GFF157 at 186.021 kb on + strand, within GFF157 at 186.022 kb on - strand, within GFF157 at 186.042 kb on + strand at 186.043 kb on - strand at 186.043 kb on - strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.064 kb on - strand at 186.064 kb on - strand at 186.064 kb on - strand at 186.078 kb on + strand at 186.078 kb on + strand at 186.116 kb on + strand at 186.116 kb on + strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.847 kb on + strand at 186.848 kb on - strand at 186.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 184,499 + GFF155 0.82 +1.1 184,500 - GFF155 0.82 -0.9 184,500 - GFF155 0.82 -0.3 184,657 + -0.3 184,657 + -0.9 184,657 + -1.0 184,657 + -0.1 184,657 + +0.8 184,658 - -0.5 184,658 - +0.6 184,658 - -0.9 184,658 - -0.1 184,658 - +1.1 184,907 + GFF156 0.15 +0.3 184,908 - GFF156 0.15 -1.1 184,908 - GFF156 0.15 -2.1 185,083 + GFF156 0.40 +1.3 185,083 + GFF156 0.40 -0.8 185,084 - GFF156 0.41 -0.6 185,084 - GFF156 0.41 +0.7 185,746 - GFF157 0.41 +1.3 185,958 + GFF157 0.76 +0.6 185,958 + GFF157 0.76 -1.0 185,959 - GFF157 0.77 +0.4 185,971 - GFF157 0.79 +0.6 185,971 - GFF157 0.79 -1.0 186,021 + GFF157 0.87 -0.1 186,022 - GFF157 0.87 -1.3 186,042 + -0.3 186,043 - -1.5 186,043 - +0.1 186,063 + +0.5 186,063 + +1.3 186,063 + +0.1 186,063 + -0.2 186,064 - -0.2 186,064 - -1.0 186,064 - -0.0 186,078 + -0.8 186,078 + -0.4 186,116 + +0.8 186,116 + -1.6 186,117 - -0.2 186,117 - -0.3 186,117 - +0.1 186,117 - -0.9 186,847 + -0.1 186,848 - -0.4 186,848 - +0.4
Or see this region's nucleotide sequence