Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1193 and GFF1194 overlap by 27 nucleotides GFF1194 and GFF1195 overlap by 4 nucleotides
GFF1193 - Adenylate cyclase (EC 4.6.1.1), at 510,497 to 512,395
GFF1193
GFF1194 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 512,369 to 513,343
GFF1194
GFF1195 - iron-sulphur protein, at 513,340 to 514,575
GFF1195
Position (kb)
512
513
514 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 511.427 kb on + strand, within GFF1193 at 511.427 kb on + strand, within GFF1193 at 511.427 kb on + strand, within GFF1193 at 511.524 kb on + strand, within GFF1193 at 511.524 kb on + strand, within GFF1193 at 511.525 kb on - strand, within GFF1193 at 511.913 kb on + strand, within GFF1193 at 511.913 kb on + strand, within GFF1193 at 511.913 kb on + strand, within GFF1193 at 511.914 kb on - strand, within GFF1193 at 512.027 kb on + strand, within GFF1193 at 512.183 kb on + strand, within GFF1193 at 512.183 kb on + strand, within GFF1193 at 512.183 kb on + strand, within GFF1193 at 512.183 kb on + strand, within GFF1193 at 512.183 kb on + strand, within GFF1193 at 512.184 kb on - strand, within GFF1193 at 512.184 kb on - strand, within GFF1193 at 512.360 kb on + strand at 512.360 kb on + strand at 512.360 kb on + strand at 512.360 kb on + strand at 512.361 kb on - strand at 512.370 kb on + strand at 512.370 kb on + strand at 512.370 kb on + strand at 512.370 kb on + strand at 512.370 kb on + strand at 512.371 kb on - strand at 512.371 kb on - strand at 512.371 kb on - strand at 512.457 kb on + strand at 512.457 kb on + strand at 512.457 kb on + strand at 512.457 kb on + strand at 512.458 kb on - strand at 512.458 kb on - strand at 512.458 kb on - strand at 512.458 kb on - strand at 512.671 kb on - strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.757 kb on + strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.758 kb on - strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.843 kb on + strand, within GFF1194 at 512.844 kb on - strand, within GFF1194 at 512.844 kb on - strand, within GFF1194 at 512.844 kb on - strand, within GFF1194 at 512.844 kb on - strand, within GFF1194 at 512.844 kb on - strand, within GFF1194 at 512.844 kb on - strand, within GFF1194 at 512.844 kb on - strand, within GFF1194 at 513.174 kb on + strand, within GFF1194 at 513.175 kb on - strand, within GFF1194 at 513.545 kb on + strand, within GFF1195 at 513.545 kb on + strand, within GFF1195 at 513.546 kb on - strand, within GFF1195 at 513.659 kb on + strand, within GFF1195 at 513.659 kb on + strand, within GFF1195 at 513.659 kb on + strand, within GFF1195 at 513.660 kb on - strand, within GFF1195 at 513.660 kb on - strand, within GFF1195 at 513.660 kb on - strand, within GFF1195 at 513.660 kb on - strand, within GFF1195 at 513.660 kb on - strand, within GFF1195 at 513.947 kb on + strand, within GFF1195 at 513.947 kb on + strand, within GFF1195 at 513.947 kb on + strand, within GFF1195 at 513.947 kb on + strand, within GFF1195 at 513.947 kb on + strand, within GFF1195 at 514.052 kb on + strand, within GFF1195 at 514.052 kb on + strand, within GFF1195 at 514.053 kb on - strand, within GFF1195 at 514.091 kb on + strand, within GFF1195 at 514.091 kb on + strand, within GFF1195 at 514.092 kb on - strand, within GFF1195 at 514.092 kb on - strand, within GFF1195 at 514.092 kb on - strand, within GFF1195 at 514.227 kb on - strand, within GFF1195 at 514.227 kb on - strand, within GFF1195 at 514.227 kb on - strand, within GFF1195 at 514.262 kb on + strand, within GFF1195 at 514.262 kb on + strand, within GFF1195 at 514.262 kb on + strand, within GFF1195 at 514.262 kb on + strand, within GFF1195 at 514.262 kb on + strand, within GFF1195 at 514.262 kb on + strand, within GFF1195 at 514.263 kb on - strand, within GFF1195 at 514.263 kb on - strand, within GFF1195 at 514.265 kb on + strand, within GFF1195 at 514.266 kb on - strand, within GFF1195
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 511,427 + GFF1193 0.49 -1.3 511,427 + GFF1193 0.49 -0.8 511,427 + GFF1193 0.49 -0.6 511,524 + GFF1193 0.54 -1.3 511,524 + GFF1193 0.54 -0.9 511,525 - GFF1193 0.54 +0.9 511,913 + GFF1193 0.75 -0.3 511,913 + GFF1193 0.75 +0.0 511,913 + GFF1193 0.75 +0.9 511,914 - GFF1193 0.75 +1.1 512,027 + GFF1193 0.81 +1.7 512,183 + GFF1193 0.89 +0.7 512,183 + GFF1193 0.89 -0.7 512,183 + GFF1193 0.89 -1.6 512,183 + GFF1193 0.89 -1.4 512,183 + GFF1193 0.89 +0.2 512,184 - GFF1193 0.89 +0.2 512,184 - GFF1193 0.89 +1.0 512,360 + +0.3 512,360 + +0.3 512,360 + +0.7 512,360 + +0.3 512,361 - +0.0 512,370 + -0.7 512,370 + +0.2 512,370 + -0.3 512,370 + +0.1 512,370 + +1.4 512,371 - -0.7 512,371 - -1.4 512,371 - -1.3 512,457 + +0.2 512,457 + -0.6 512,457 + +0.1 512,457 + -0.0 512,458 - -0.4 512,458 - +0.1 512,458 - -0.6 512,458 - -0.5 512,671 - GFF1194 0.31 -0.5 512,757 + GFF1194 0.40 -1.4 512,757 + GFF1194 0.40 +0.8 512,757 + GFF1194 0.40 +0.5 512,757 + GFF1194 0.40 -1.2 512,757 + GFF1194 0.40 +0.0 512,757 + GFF1194 0.40 -2.4 512,757 + GFF1194 0.40 +0.1 512,757 + GFF1194 0.40 -0.6 512,757 + GFF1194 0.40 -0.5 512,757 + GFF1194 0.40 -0.2 512,757 + GFF1194 0.40 +0.2 512,758 - GFF1194 0.40 +1.7 512,758 - GFF1194 0.40 -2.1 512,758 - GFF1194 0.40 -0.2 512,758 - GFF1194 0.40 -0.8 512,758 - GFF1194 0.40 +0.4 512,758 - GFF1194 0.40 +1.4 512,758 - GFF1194 0.40 +0.9 512,758 - GFF1194 0.40 +0.0 512,758 - GFF1194 0.40 +0.8 512,758 - GFF1194 0.40 -0.2 512,758 - GFF1194 0.40 -0.5 512,758 - GFF1194 0.40 -0.9 512,758 - GFF1194 0.40 +0.2 512,843 + GFF1194 0.49 -0.9 512,843 + GFF1194 0.49 -0.5 512,843 + GFF1194 0.49 +0.4 512,843 + GFF1194 0.49 -1.7 512,843 + GFF1194 0.49 -0.4 512,843 + GFF1194 0.49 -0.6 512,843 + GFF1194 0.49 -0.2 512,843 + GFF1194 0.49 +0.8 512,844 - GFF1194 0.49 +0.1 512,844 - GFF1194 0.49 -0.4 512,844 - GFF1194 0.49 -0.0 512,844 - GFF1194 0.49 -0.5 512,844 - GFF1194 0.49 +0.3 512,844 - GFF1194 0.49 -0.9 512,844 - GFF1194 0.49 -0.8 513,174 + GFF1194 0.83 -0.9 513,175 - GFF1194 0.83 +1.4 513,545 + GFF1195 0.17 +0.8 513,545 + GFF1195 0.17 -0.9 513,546 - GFF1195 0.17 +0.6 513,659 + GFF1195 0.26 +0.5 513,659 + GFF1195 0.26 -0.2 513,659 + GFF1195 0.26 +0.9 513,660 - GFF1195 0.26 -0.1 513,660 - GFF1195 0.26 -0.5 513,660 - GFF1195 0.26 +0.6 513,660 - GFF1195 0.26 -0.3 513,660 - GFF1195 0.26 +1.7 513,947 + GFF1195 0.49 -0.3 513,947 + GFF1195 0.49 -1.0 513,947 + GFF1195 0.49 -0.3 513,947 + GFF1195 0.49 +0.5 513,947 + GFF1195 0.49 +0.1 514,052 + GFF1195 0.58 -0.5 514,052 + GFF1195 0.58 -0.1 514,053 - GFF1195 0.58 -0.1 514,091 + GFF1195 0.61 -1.4 514,091 + GFF1195 0.61 -0.1 514,092 - GFF1195 0.61 +0.2 514,092 - GFF1195 0.61 -2.9 514,092 - GFF1195 0.61 +0.0 514,227 - GFF1195 0.72 +0.7 514,227 - GFF1195 0.72 -1.4 514,227 - GFF1195 0.72 -0.6 514,262 + GFF1195 0.75 -1.6 514,262 + GFF1195 0.75 -0.4 514,262 + GFF1195 0.75 -0.4 514,262 + GFF1195 0.75 +0.4 514,262 + GFF1195 0.75 -0.5 514,262 + GFF1195 0.75 -2.1 514,263 - GFF1195 0.75 -3.1 514,263 - GFF1195 0.75 +0.1 514,265 + GFF1195 0.75 -0.7 514,266 - GFF1195 0.75 +0.4
Or see this region's nucleotide sequence