Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF113 and GFF114 overlap by 11 nucleotides GFF114 and GFF115 are separated by 1 nucleotides GFF115 and GFF116 are separated by 3 nucleotides GFF116 and GFF117 are separated by 188 nucleotides
GFF113 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 142,023 to 142,742
GFF113
GFF114 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 142,732 to 143,388
GFF114
GFF115 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 143,390 to 144,055
GFF115
GFF116 - RidA/YER057c/UK114 superfamily, group 1, at 144,059 to 144,571
GFF116
GFF117 - no description, at 144,760 to 145,539
GFF117
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 142.698 kb on + strand at 142.698 kb on + strand at 142.698 kb on + strand at 142.698 kb on + strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.808 kb on + strand, within GFF114 at 142.808 kb on + strand, within GFF114 at 142.809 kb on - strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.941 kb on - strand, within GFF114 at 142.941 kb on - strand, within GFF114 at 143.174 kb on + strand, within GFF114 at 143.174 kb on + strand, within GFF114 at 143.225 kb on + strand, within GFF114 at 143.225 kb on + strand, within GFF114 at 143.225 kb on + strand, within GFF114 at 143.225 kb on + strand, within GFF114 at 143.225 kb on + strand, within GFF114 at 143.225 kb on + strand, within GFF114 at 143.225 kb on + strand, within GFF114 at 143.226 kb on - strand, within GFF114 at 143.226 kb on - strand, within GFF114 at 143.226 kb on - strand, within GFF114 at 143.463 kb on + strand, within GFF115 at 143.463 kb on + strand, within GFF115 at 143.464 kb on - strand, within GFF115 at 143.464 kb on - strand, within GFF115 at 143.464 kb on - strand, within GFF115 at 143.484 kb on + strand, within GFF115 at 143.484 kb on + strand, within GFF115 at 143.485 kb on - strand, within GFF115 at 143.485 kb on - strand, within GFF115 at 143.732 kb on + strand, within GFF115 at 143.745 kb on + strand, within GFF115 at 143.745 kb on + strand, within GFF115 at 143.745 kb on + strand, within GFF115 at 143.746 kb on - strand, within GFF115 at 143.746 kb on - strand, within GFF115 at 143.746 kb on - strand, within GFF115 at 143.827 kb on - strand, within GFF115 at 143.827 kb on - strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.977 kb on - strand, within GFF115 at 143.977 kb on - strand, within GFF115 at 144.019 kb on - strand at 144.048 kb on + strand at 144.048 kb on + strand at 144.333 kb on + strand, within GFF116 at 144.333 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.712 kb on - strand at 144.872 kb on - strand, within GFF117
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 142,698 + -0.9 142,698 + -0.3 142,698 + +0.2 142,698 + +0.2 142,699 - -1.1 142,699 - -1.4 142,699 - -2.6 142,699 - +0.5 142,699 - +0.4 142,699 - -0.0 142,699 - -0.9 142,808 + GFF114 0.12 -0.1 142,808 + GFF114 0.12 -0.9 142,809 - GFF114 0.12 +0.4 142,940 + GFF114 0.32 -0.1 142,940 + GFF114 0.32 -0.4 142,940 + GFF114 0.32 +0.5 142,940 + GFF114 0.32 +0.7 142,940 + GFF114 0.32 -0.9 142,941 - GFF114 0.32 -0.3 142,941 - GFF114 0.32 +0.1 143,174 + GFF114 0.67 -0.8 143,174 + GFF114 0.67 -0.1 143,225 + GFF114 0.75 -1.7 143,225 + GFF114 0.75 -1.9 143,225 + GFF114 0.75 +0.7 143,225 + GFF114 0.75 -0.3 143,225 + GFF114 0.75 +1.0 143,225 + GFF114 0.75 +0.3 143,225 + GFF114 0.75 -0.4 143,226 - GFF114 0.75 -0.1 143,226 - GFF114 0.75 -1.0 143,226 - GFF114 0.75 -0.6 143,463 + GFF115 0.11 -0.7 143,463 + GFF115 0.11 +0.2 143,464 - GFF115 0.11 +0.3 143,464 - GFF115 0.11 -0.2 143,464 - GFF115 0.11 -0.9 143,484 + GFF115 0.14 -1.7 143,484 + GFF115 0.14 -1.0 143,485 - GFF115 0.14 +0.2 143,485 - GFF115 0.14 +0.7 143,732 + GFF115 0.51 -0.7 143,745 + GFF115 0.53 -1.3 143,745 + GFF115 0.53 +0.2 143,745 + GFF115 0.53 -0.8 143,746 - GFF115 0.53 -0.2 143,746 - GFF115 0.53 -0.2 143,746 - GFF115 0.53 +0.2 143,827 - GFF115 0.66 +1.1 143,827 - GFF115 0.66 -0.7 143,877 + GFF115 0.73 +0.5 143,877 + GFF115 0.73 +0.3 143,877 + GFF115 0.73 +1.3 143,877 + GFF115 0.73 +0.4 143,877 + GFF115 0.73 -0.2 143,877 + GFF115 0.73 +0.3 143,877 + GFF115 0.73 +0.2 143,878 - GFF115 0.73 +0.1 143,878 - GFF115 0.73 -1.7 143,878 - GFF115 0.73 +1.3 143,878 - GFF115 0.73 -0.2 143,977 - GFF115 0.88 +1.0 143,977 - GFF115 0.88 -1.3 144,019 - -0.5 144,048 + -1.1 144,048 + +0.1 144,333 + GFF116 0.53 +0.3 144,333 + GFF116 0.53 -0.9 144,417 + GFF116 0.70 +0.1 144,417 + GFF116 0.70 -1.1 144,417 + GFF116 0.70 +1.2 144,417 + GFF116 0.70 +0.4 144,417 + GFF116 0.70 +0.3 144,418 - GFF116 0.70 -0.9 144,418 - GFF116 0.70 -1.3 144,418 - GFF116 0.70 -0.7 144,418 - GFF116 0.70 -0.7 144,418 - GFF116 0.70 -0.2 144,685 + -2.8 144,685 + +0.5 144,685 + -0.4 144,685 + +0.4 144,685 + +0.9 144,685 + -0.4 144,685 + -0.2 144,686 - -1.4 144,686 - -0.5 144,686 - -1.1 144,686 - -0.1 144,686 - +0.0 144,686 - -0.3 144,686 - +1.3 144,686 - +0.1 144,712 - -0.2 144,872 - GFF117 0.14 -0.3
Or see this region's nucleotide sequence