Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1106 and GFF1107 overlap by 4 nucleotides GFF1107 and GFF1108 are separated by 367 nucleotides
GFF1106 - UvrD/REP helicase, at 420,691 to 423,951
GFF1106
GFF1107 - NAD(FAD)-utilizing dehydrogenases, at 423,948 to 425,186
GFF1107
GFF1108 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 425,554 to 427,425
GFF1108
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -2
-1
0
1
2 at 423.259 kb on + strand, within GFF1106 at 423.259 kb on + strand, within GFF1106 at 423.260 kb on - strand, within GFF1106 at 423.887 kb on - strand at 423.938 kb on + strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.013 kb on - strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.044 kb on + strand at 424.045 kb on - strand at 424.045 kb on - strand at 424.045 kb on - strand at 424.045 kb on - strand at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.337 kb on + strand, within GFF1107 at 424.338 kb on - strand, within GFF1107 at 424.542 kb on - strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.704 kb on + strand, within GFF1107 at 424.705 kb on - strand, within GFF1107 at 424.705 kb on - strand, within GFF1107 at 424.877 kb on + strand, within GFF1107 at 424.877 kb on + strand, within GFF1107 at 424.878 kb on - strand, within GFF1107 at 424.878 kb on - strand, within GFF1107 at 425.115 kb on + strand at 425.116 kb on - strand at 425.166 kb on - strand at 425.184 kb on + strand at 425.184 kb on + strand at 425.184 kb on + strand at 425.184 kb on + strand at 425.185 kb on - strand at 425.185 kb on - strand at 425.185 kb on - strand at 425.382 kb on + strand at 425.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 423,259 + GFF1106 0.79 +1.1 423,259 + GFF1106 0.79 -0.5 423,260 - GFF1106 0.79 +1.9 423,887 - -1.7 423,938 + -0.9 424,013 - +0.8 424,013 - -1.0 424,013 - +0.1 424,013 - -0.8 424,013 - -0.6 424,044 + -0.7 424,044 + +0.1 424,044 + +0.3 424,044 + +0.2 424,044 + +0.8 424,044 + -0.4 424,045 - +1.4 424,045 - -0.6 424,045 - +0.1 424,045 - -0.3 424,337 + GFF1107 0.31 -1.9 424,337 + GFF1107 0.31 -0.3 424,337 + GFF1107 0.31 -1.6 424,337 + GFF1107 0.31 +0.4 424,337 + GFF1107 0.31 -0.9 424,338 - GFF1107 0.31 -0.1 424,542 - GFF1107 0.48 +0.3 424,704 + GFF1107 0.61 +0.8 424,704 + GFF1107 0.61 +0.1 424,704 + GFF1107 0.61 -0.2 424,704 + GFF1107 0.61 +0.0 424,704 + GFF1107 0.61 +0.6 424,704 + GFF1107 0.61 +0.0 424,704 + GFF1107 0.61 +0.4 424,705 - GFF1107 0.61 +0.1 424,705 - GFF1107 0.61 -0.2 424,877 + GFF1107 0.75 +0.4 424,877 + GFF1107 0.75 +0.9 424,878 - GFF1107 0.75 -1.4 424,878 - GFF1107 0.75 -1.4 425,115 + -0.2 425,116 - -1.0 425,166 - -0.6 425,184 + -1.3 425,184 + -1.4 425,184 + -1.2 425,184 + -0.3 425,185 - -0.5 425,185 - +0.2 425,185 - +0.4 425,382 + -1.3 425,383 - -0.4
Or see this region's nucleotide sequence