Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF964 and GFF965 are separated by 76 nucleotides GFF965 and GFF966 overlap by 4 nucleotides GFF966 and GFF967 are separated by 219 nucleotides
GFF964 - Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) / Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5), at 275,813 to 276,664
GFF964
GFF965 - Two-component transcriptional response regulator, LuxR family, at 276,741 to 277,373
GFF965
GFF966 - Probable two-component transmembrane sensor histidine kinase transcription regulator protein (EC 2.7.3.-), at 277,370 to 279,910
GFF966
GFF967 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 280,130 to 282,844
GFF967
Position (kb) 277
278
279
280 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 276.683 kb on + strand at 276.735 kb on + strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.743 kb on - strand at 276.743 kb on - strand at 276.743 kb on - strand at 277.235 kb on - strand, within GFF965 at 277.280 kb on - strand, within GFF965 at 277.343 kb on - strand at 277.343 kb on - strand at 277.380 kb on - strand at 277.380 kb on - strand at 277.380 kb on - strand at 277.397 kb on - strand at 277.397 kb on - strand at 277.397 kb on - strand at 277.397 kb on - strand at 277.400 kb on + strand at 277.471 kb on - strand at 277.471 kb on - strand at 277.471 kb on - strand at 277.576 kb on - strand at 278.047 kb on - strand, within GFF966 at 278.047 kb on - strand, within GFF966 at 278.538 kb on + strand, within GFF966 at 278.539 kb on - strand, within GFF966 at 278.539 kb on - strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.643 kb on + strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.644 kb on - strand, within GFF966 at 278.772 kb on + strand, within GFF966 at 278.847 kb on + strand, within GFF966 at 278.847 kb on + strand, within GFF966 at 278.847 kb on + strand, within GFF966 at 278.847 kb on + strand, within GFF966 at 278.848 kb on - strand, within GFF966 at 278.848 kb on - strand, within GFF966 at 278.848 kb on - strand, within GFF966 at 278.848 kb on - strand, within GFF966 at 278.848 kb on - strand, within GFF966 at 278.915 kb on + strand, within GFF966 at 279.117 kb on + strand, within GFF966 at 279.118 kb on - strand, within GFF966 at 279.118 kb on - strand, within GFF966 at 279.172 kb on + strand, within GFF966 at 279.172 kb on + strand, within GFF966 at 279.173 kb on - strand, within GFF966 at 279.175 kb on + strand, within GFF966 at 279.175 kb on + strand, within GFF966 at 279.176 kb on - strand, within GFF966 at 279.176 kb on - strand, within GFF966 at 279.176 kb on - strand, within GFF966 at 279.226 kb on - strand, within GFF966 at 279.226 kb on - strand, within GFF966 at 279.226 kb on - strand, within GFF966 at 279.256 kb on - strand, within GFF966 at 279.378 kb on + strand, within GFF966 at 279.378 kb on + strand, within GFF966 at 279.379 kb on - strand, within GFF966 at 279.379 kb on - strand, within GFF966 at 279.411 kb on + strand, within GFF966 at 279.411 kb on + strand, within GFF966 at 279.411 kb on + strand, within GFF966 at 279.412 kb on - strand, within GFF966 at 279.695 kb on + strand at 279.696 kb on - strand at 279.696 kb on - strand at 279.728 kb on - strand at 279.735 kb on + strand at 279.921 kb on + strand at 279.921 kb on + strand at 279.921 kb on + strand at 279.921 kb on + strand at 279.921 kb on + strand at 279.921 kb on + strand at 279.922 kb on - strand at 279.922 kb on - strand at 279.984 kb on + strand at 279.984 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.988 kb on + strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 279.989 kb on - strand at 280.038 kb on - strand at 280.053 kb on - strand at 280.053 kb on - strand at 280.056 kb on + strand at 280.056 kb on + strand at 280.058 kb on + strand at 280.058 kb on + strand at 280.058 kb on + strand at 280.059 kb on + strand at 280.059 kb on - strand at 280.086 kb on + strand at 280.086 kb on + strand at 280.086 kb on + strand at 280.086 kb on + strand at 280.086 kb on + strand at 280.087 kb on - strand at 280.087 kb on - strand at 280.094 kb on + strand at 280.094 kb on + strand at 280.095 kb on - strand at 280.095 kb on - strand at 280.095 kb on - strand at 280.095 kb on - strand at 280.095 kb on - strand at 280.095 kb on - strand at 280.095 kb on - strand at 280.095 kb on - strand at 280.114 kb on - strand at 280.325 kb on + strand at 280.325 kb on + strand at 280.325 kb on + strand at 280.325 kb on + strand at 280.325 kb on + strand at 280.326 kb on - strand at 280.326 kb on - strand at 280.326 kb on - strand at 280.326 kb on - strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.574 kb on + strand, within GFF967 at 280.575 kb on - strand, within GFF967 at 280.575 kb on - strand, within GFF967 at 280.575 kb on - strand, within GFF967 at 280.575 kb on - strand, within GFF967 at 280.575 kb on - strand, within GFF967 at 280.575 kb on - strand, within GFF967 at 280.575 kb on - strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.604 kb on + strand, within GFF967 at 280.605 kb on - strand, within GFF967 at 280.605 kb on - strand, within GFF967 at 280.605 kb on - strand, within GFF967 at 280.605 kb on - strand, within GFF967 at 280.616 kb on + strand, within GFF967 at 280.769 kb on + strand, within GFF967 at 280.769 kb on + strand, within GFF967 at 280.769 kb on + strand, within GFF967 at 280.769 kb on + strand, within GFF967 at 280.769 kb on + strand, within GFF967 at 280.769 kb on + strand, within GFF967 at 280.769 kb on + strand, within GFF967 at 280.770 kb on - strand, within GFF967 at 280.770 kb on - strand, within GFF967 at 280.770 kb on - strand, within GFF967 at 280.770 kb on - strand, within GFF967 at 280.770 kb on - strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.790 kb on + strand, within GFF967 at 280.791 kb on - strand, within GFF967
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 276,683 + +0.5 276,735 + +1.5 276,736 - +0.1 276,736 - +0.3 276,736 - -0.7 276,736 - +0.2 276,736 - +0.0 276,736 - -0.3 276,736 - -0.2 276,742 + +1.0 276,742 + -0.3 276,742 + +0.5 276,742 + -0.9 276,742 + +0.1 276,742 + +1.9 276,742 + +0.1 276,742 + +0.5 276,743 - -0.1 276,743 - -0.4 276,743 - -0.1 277,235 - GFF965 0.78 -1.1 277,280 - GFF965 0.85 +1.5 277,343 - -2.2 277,343 - -0.5 277,380 - +1.5 277,380 - -0.1 277,380 - -0.5 277,397 - -0.3 277,397 - +0.4 277,397 - -0.4 277,397 - +0.4 277,400 + +0.5 277,471 - -0.5 277,471 - -1.5 277,471 - -1.3 277,576 - -2.1 278,047 - GFF966 0.27 -2.7 278,047 - GFF966 0.27 -1.7 278,538 + GFF966 0.46 -0.8 278,539 - GFF966 0.46 -2.1 278,539 - GFF966 0.46 -1.5 278,643 + GFF966 0.50 -2.4 278,643 + GFF966 0.50 -0.5 278,643 + GFF966 0.50 -1.8 278,643 + GFF966 0.50 -1.5 278,643 + GFF966 0.50 -2.1 278,643 + GFF966 0.50 -0.8 278,643 + GFF966 0.50 -0.3 278,643 + GFF966 0.50 -1.8 278,643 + GFF966 0.50 -2.2 278,643 + GFF966 0.50 -0.8 278,643 + GFF966 0.50 -0.2 278,644 - GFF966 0.50 -1.8 278,644 - GFF966 0.50 -1.3 278,644 - GFF966 0.50 -1.8 278,644 - GFF966 0.50 -0.3 278,644 - GFF966 0.50 -2.2 278,644 - GFF966 0.50 -1.5 278,644 - GFF966 0.50 -1.7 278,644 - GFF966 0.50 -2.1 278,644 - GFF966 0.50 -2.3 278,644 - GFF966 0.50 -1.4 278,772 + GFF966 0.55 -0.5 278,847 + GFF966 0.58 +0.5 278,847 + GFF966 0.58 -0.8 278,847 + GFF966 0.58 -2.4 278,847 + GFF966 0.58 -1.3 278,848 - GFF966 0.58 -1.5 278,848 - GFF966 0.58 -1.8 278,848 - GFF966 0.58 -0.5 278,848 - GFF966 0.58 -1.9 278,848 - GFF966 0.58 -1.7 278,915 + GFF966 0.61 -1.5 279,117 + GFF966 0.69 -1.9 279,118 - GFF966 0.69 -2.6 279,118 - GFF966 0.69 -0.7 279,172 + GFF966 0.71 -1.7 279,172 + GFF966 0.71 -0.5 279,173 - GFF966 0.71 -1.3 279,175 + GFF966 0.71 -1.9 279,175 + GFF966 0.71 -1.9 279,176 - GFF966 0.71 -1.5 279,176 - GFF966 0.71 -0.8 279,176 - GFF966 0.71 -1.1 279,226 - GFF966 0.73 +0.3 279,226 - GFF966 0.73 -0.7 279,226 - GFF966 0.73 -1.5 279,256 - GFF966 0.74 -2.1 279,378 + GFF966 0.79 -0.3 279,378 + GFF966 0.79 -2.5 279,379 - GFF966 0.79 -1.9 279,379 - GFF966 0.79 -1.7 279,411 + GFF966 0.80 -0.1 279,411 + GFF966 0.80 -1.1 279,411 + GFF966 0.80 -1.3 279,412 - GFF966 0.80 -0.5 279,695 + -2.6 279,696 - -0.8 279,696 - -0.5 279,728 - -0.5 279,735 + -1.9 279,921 + -0.5 279,921 + +0.2 279,921 + -0.4 279,921 + -0.2 279,921 + -1.1 279,921 + -0.4 279,922 - -0.6 279,922 - +0.4 279,984 + +0.3 279,984 + -1.1 279,988 + +0.1 279,988 + -1.8 279,988 + -0.1 279,988 + -1.4 279,988 + -0.4 279,988 + -0.6 279,988 + -0.6 279,988 + -0.1 279,988 + -0.0 279,988 + -2.9 279,988 + +0.0 279,989 - +0.4 279,989 - +0.6 279,989 - +0.6 279,989 - -1.6 279,989 - -0.6 279,989 - -0.8 279,989 - -0.6 279,989 - -0.3 279,989 - -0.3 279,989 - -1.0 279,989 - -0.8 279,989 - +0.0 279,989 - +0.4 279,989 - -0.2 279,989 - +0.2 279,989 - +0.0 279,989 - -1.1 279,989 - +0.9 279,989 - -0.5 279,989 - -1.2 280,038 - +0.2 280,053 - +0.6 280,053 - -2.3 280,056 + +0.4 280,056 + -0.0 280,058 + -0.3 280,058 + -0.5 280,058 + +1.3 280,059 + +0.0 280,059 - -0.5 280,086 + +0.0 280,086 + -0.3 280,086 + -0.3 280,086 + -0.8 280,086 + +1.0 280,087 - -1.6 280,087 - -0.6 280,094 + -1.2 280,094 + +0.6 280,095 - +0.6 280,095 - -0.2 280,095 - -0.4 280,095 - -1.0 280,095 - +0.0 280,095 - -0.1 280,095 - -0.8 280,095 - -1.3 280,114 - -0.8 280,325 + -1.8 280,325 + +0.5 280,325 + -0.1 280,325 + -0.5 280,325 + -0.5 280,326 - -1.1 280,326 - -2.1 280,326 - -0.1 280,326 - -2.3 280,400 + +0.1 280,400 + -1.3 280,400 + -1.6 280,400 + -0.8 280,400 + -2.6 280,401 - -0.1 280,401 - -0.5 280,401 - -1.5 280,401 - -0.5 280,401 - -0.1 280,401 - -0.5 280,401 - -0.1 280,574 + GFF967 0.16 -0.5 280,574 + GFF967 0.16 -0.1 280,574 + GFF967 0.16 -1.5 280,574 + GFF967 0.16 -0.8 280,574 + GFF967 0.16 +1.1 280,574 + GFF967 0.16 -3.0 280,574 + GFF967 0.16 -2.3 280,574 + GFF967 0.16 -1.9 280,574 + GFF967 0.16 -1.4 280,574 + GFF967 0.16 -1.1 280,574 + GFF967 0.16 -2.5 280,574 + GFF967 0.16 -0.7 280,575 - GFF967 0.16 +1.5 280,575 - GFF967 0.16 -1.5 280,575 - GFF967 0.16 -0.1 280,575 - GFF967 0.16 -1.1 280,575 - GFF967 0.16 -3.1 280,575 - GFF967 0.16 -1.8 280,575 - GFF967 0.16 -2.2 280,604 + GFF967 0.17 -2.5 280,604 + GFF967 0.17 -0.5 280,604 + GFF967 0.17 -0.1 280,604 + GFF967 0.17 +0.5 280,604 + GFF967 0.17 -1.5 280,604 + GFF967 0.17 -1.8 280,604 + GFF967 0.17 +0.2 280,604 + GFF967 0.17 -0.5 280,605 - GFF967 0.17 -1.2 280,605 - GFF967 0.17 -1.7 280,605 - GFF967 0.17 -0.8 280,605 - GFF967 0.17 -1.5 280,616 + GFF967 0.18 -0.8 280,769 + GFF967 0.24 -0.8 280,769 + GFF967 0.24 -0.5 280,769 + GFF967 0.24 -1.7 280,769 + GFF967 0.24 -1.7 280,769 + GFF967 0.24 +1.5 280,769 + GFF967 0.24 -1.1 280,769 + GFF967 0.24 -0.2 280,770 - GFF967 0.24 -1.3 280,770 - GFF967 0.24 -2.1 280,770 - GFF967 0.24 -0.1 280,770 - GFF967 0.24 -1.5 280,770 - GFF967 0.24 -0.7 280,790 + GFF967 0.24 -1.3 280,790 + GFF967 0.24 -1.7 280,790 + GFF967 0.24 -1.7 280,790 + GFF967 0.24 -1.8 280,790 + GFF967 0.24 -1.2 280,790 + GFF967 0.24 -1.3 280,790 + GFF967 0.24 -1.8 280,790 + GFF967 0.24 -0.1 280,791 - GFF967 0.24 -0.5
Or see this region's nucleotide sequence