Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF945 and GFF946 are separated by 242 nucleotides GFF946 and GFF947 are separated by 100 nucleotides
GFF945 - Excinuclease ABC subunit B, at 258,698 to 260,806
GFF945
GFF946 - Iron-sulfur cluster regulator IscR, at 261,049 to 261,585
GFF946
GFF947 - Cysteine desulfurase (EC 2.8.1.7) => IscS, at 261,686 to 262,906
GFF947
Position (kb)
261
262 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.082 kb on + strand, within GFF945 at 260.083 kb on - strand, within GFF945 at 260.083 kb on - strand, within GFF945 at 260.083 kb on - strand, within GFF945 at 260.083 kb on - strand, within GFF945 at 260.100 kb on + strand, within GFF945 at 260.100 kb on + strand, within GFF945 at 260.100 kb on + strand, within GFF945 at 260.101 kb on - strand, within GFF945 at 260.171 kb on + strand, within GFF945 at 260.171 kb on + strand, within GFF945 at 260.172 kb on - strand, within GFF945 at 260.172 kb on - strand, within GFF945 at 260.172 kb on - strand, within GFF945 at 260.201 kb on + strand, within GFF945 at 260.202 kb on - strand, within GFF945 at 260.202 kb on - strand, within GFF945 at 260.202 kb on - strand, within GFF945 at 260.202 kb on - strand, within GFF945 at 260.202 kb on - strand, within GFF945 at 260.241 kb on + strand, within GFF945 at 260.241 kb on + strand, within GFF945 at 260.241 kb on + strand, within GFF945 at 260.241 kb on + strand, within GFF945 at 260.241 kb on + strand, within GFF945 at 260.241 kb on + strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.242 kb on - strand, within GFF945 at 260.312 kb on - strand, within GFF945 at 260.435 kb on + strand, within GFF945 at 260.435 kb on + strand, within GFF945 at 260.435 kb on + strand, within GFF945 at 260.436 kb on - strand, within GFF945 at 260.436 kb on - strand, within GFF945 at 260.579 kb on + strand, within GFF945 at 260.579 kb on + strand, within GFF945 at 260.579 kb on + strand, within GFF945 at 260.579 kb on + strand, within GFF945 at 260.580 kb on - strand, within GFF945 at 260.580 kb on - strand, within GFF945 at 260.580 kb on - strand, within GFF945 at 260.580 kb on - strand, within GFF945 at 260.600 kb on + strand at 260.804 kb on + strand at 260.804 kb on + strand at 260.804 kb on + strand at 260.804 kb on + strand at 260.804 kb on + strand at 260.805 kb on - strand at 260.805 kb on - strand at 260.805 kb on - strand at 260.805 kb on - strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.857 kb on - strand at 260.884 kb on + strand at 260.885 kb on - strand at 260.885 kb on - strand at 260.885 kb on - strand at 260.886 kb on + strand at 260.886 kb on + strand at 260.944 kb on - strand at 260.944 kb on - strand at 261.027 kb on + strand at 261.027 kb on + strand at 261.169 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.241 kb on + strand, within GFF946 at 261.241 kb on + strand, within GFF946
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 260,082 + GFF945 0.66 -0.6 260,082 + GFF945 0.66 +0.8 260,082 + GFF945 0.66 -0.2 260,082 + GFF945 0.66 -0.4 260,082 + GFF945 0.66 +0.2 260,082 + GFF945 0.66 -0.5 260,082 + GFF945 0.66 -0.2 260,082 + GFF945 0.66 -0.2 260,082 + GFF945 0.66 +0.7 260,083 - GFF945 0.66 +0.5 260,083 - GFF945 0.66 -0.6 260,083 - GFF945 0.66 -2.4 260,083 - GFF945 0.66 -1.0 260,100 + GFF945 0.66 +0.7 260,100 + GFF945 0.66 +0.9 260,100 + GFF945 0.66 -1.5 260,101 - GFF945 0.67 -2.1 260,171 + GFF945 0.70 -0.1 260,171 + GFF945 0.70 -0.1 260,172 - GFF945 0.70 -1.1 260,172 - GFF945 0.70 -0.4 260,172 - GFF945 0.70 -0.8 260,201 + GFF945 0.71 +0.6 260,202 - GFF945 0.71 -0.4 260,202 - GFF945 0.71 +0.0 260,202 - GFF945 0.71 -0.1 260,202 - GFF945 0.71 -0.9 260,202 - GFF945 0.71 -0.4 260,241 + GFF945 0.73 -0.1 260,241 + GFF945 0.73 -1.3 260,241 + GFF945 0.73 -0.4 260,241 + GFF945 0.73 -0.1 260,241 + GFF945 0.73 -0.4 260,241 + GFF945 0.73 -0.4 260,242 - GFF945 0.73 -0.6 260,242 - GFF945 0.73 -0.8 260,242 - GFF945 0.73 +0.1 260,242 - GFF945 0.73 -0.1 260,242 - GFF945 0.73 +0.6 260,242 - GFF945 0.73 -0.8 260,242 - GFF945 0.73 -0.0 260,242 - GFF945 0.73 -0.5 260,242 - GFF945 0.73 +1.2 260,242 - GFF945 0.73 -0.7 260,242 - GFF945 0.73 -0.3 260,312 - GFF945 0.77 +1.0 260,435 + GFF945 0.82 +0.1 260,435 + GFF945 0.82 -0.8 260,435 + GFF945 0.82 +0.7 260,436 - GFF945 0.82 -1.1 260,436 - GFF945 0.82 -1.1 260,579 + GFF945 0.89 -0.8 260,579 + GFF945 0.89 -0.3 260,579 + GFF945 0.89 -0.6 260,579 + GFF945 0.89 -0.4 260,580 - GFF945 0.89 -0.3 260,580 - GFF945 0.89 -0.4 260,580 - GFF945 0.89 -0.2 260,580 - GFF945 0.89 +0.2 260,600 + +0.5 260,804 + -1.1 260,804 + -0.7 260,804 + +0.2 260,804 + -1.7 260,804 + +0.3 260,805 - -1.3 260,805 - -0.3 260,805 - +0.1 260,805 - +0.9 260,813 + -0.4 260,813 + -0.1 260,813 + -0.1 260,813 + -0.2 260,813 + +0.1 260,813 + +0.1 260,814 - -0.2 260,814 - -0.5 260,814 - +1.5 260,814 - -0.0 260,814 - +0.1 260,814 - -1.0 260,814 - -1.1 260,857 - +0.1 260,884 + -0.9 260,885 - -1.1 260,885 - -0.0 260,885 - -1.1 260,886 + -1.3 260,886 + -0.2 260,944 - -0.4 260,944 - -1.3 261,027 + -1.8 261,027 + +0.2 261,169 + GFF946 0.22 -1.5 261,209 + GFF946 0.30 -3.6 261,209 + GFF946 0.30 -1.1 261,209 + GFF946 0.30 -2.0 261,209 + GFF946 0.30 -1.3 261,241 + GFF946 0.36 -3.1 261,241 + GFF946 0.36 -0.1
Or see this region's nucleotide sequence