Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF904 and GFF905 are separated by 8 nucleotides GFF905 and GFF906 are separated by 11 nucleotides GFF906 and GFF907 are separated by 109 nucleotides
GFF904 - hypothetical protein, at 215,727 to 216,158
GFF904
GFF905 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 216,167 to 216,943
GFF905
GFF906 - Transcriptional regulator, at 216,955 to 217,422
GFF906
GFF907 - Transcriptional regulator, LysR family, at 217,532 to 218,425
GFF907
Position (kb) 216
217
218 Strain fitness (log2 ratio) -2
-1
0
1 at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.430 kb on - strand, within GFF905 at 216.551 kb on + strand, within GFF905 at 216.551 kb on + strand, within GFF905 at 216.552 kb on - strand, within GFF905 at 216.568 kb on - strand, within GFF905 at 216.587 kb on + strand, within GFF905 at 216.587 kb on + strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.618 kb on + strand, within GFF905 at 216.690 kb on + strand, within GFF905 at 216.690 kb on + strand, within GFF905 at 216.782 kb on + strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.811 kb on - strand, within GFF905 at 216.811 kb on - strand, within GFF905 at 216.965 kb on + strand at 216.966 kb on - strand at 217.229 kb on + strand, within GFF906 at 217.229 kb on + strand, within GFF906 at 217.229 kb on + strand, within GFF906 at 217.230 kb on - strand, within GFF906 at 217.230 kb on - strand, within GFF906 at 217.230 kb on - strand, within GFF906 at 217.434 kb on + strand at 217.434 kb on + strand at 217.434 kb on + strand at 217.434 kb on + strand at 217.435 kb on - strand at 217.435 kb on - strand at 217.436 kb on + strand at 217.437 kb on - strand at 217.437 kb on - strand at 217.437 kb on - strand at 217.458 kb on - strand at 217.599 kb on + strand at 217.599 kb on + strand at 217.599 kb on + strand at 217.815 kb on + strand, within GFF907 at 217.815 kb on + strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 217.816 kb on - strand, within GFF907 at 218.068 kb on - strand, within GFF907 at 218.068 kb on - strand, within GFF907
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 216,237 + +0.6 216,237 + -0.3 216,237 + -0.1 216,237 + +0.3 216,237 + -0.4 216,237 + +0.5 216,237 + +0.1 216,237 + +1.5 216,238 - -0.3 216,238 - +0.8 216,238 - +0.8 216,238 - -1.3 216,238 - -1.3 216,430 - GFF905 0.34 +0.3 216,551 + GFF905 0.49 +0.2 216,551 + GFF905 0.49 +0.5 216,552 - GFF905 0.50 -0.3 216,568 - GFF905 0.52 +0.5 216,587 + GFF905 0.54 +0.2 216,587 + GFF905 0.54 +1.0 216,588 - GFF905 0.54 -0.7 216,588 - GFF905 0.54 +0.2 216,588 - GFF905 0.54 -0.1 216,618 + GFF905 0.58 +0.4 216,690 + GFF905 0.67 -2.4 216,690 + GFF905 0.67 -0.2 216,782 + GFF905 0.79 +0.4 216,783 - GFF905 0.79 +0.5 216,783 - GFF905 0.79 -0.1 216,783 - GFF905 0.79 -0.9 216,783 - GFF905 0.79 +0.9 216,811 - GFF905 0.83 -0.1 216,811 - GFF905 0.83 -0.6 216,965 + +0.7 216,966 - -1.7 217,229 + GFF906 0.59 -0.9 217,229 + GFF906 0.59 -0.7 217,229 + GFF906 0.59 +1.1 217,230 - GFF906 0.59 -0.8 217,230 - GFF906 0.59 -1.8 217,230 - GFF906 0.59 -0.4 217,434 + -0.1 217,434 + -0.1 217,434 + -0.9 217,434 + -0.1 217,435 - -0.2 217,435 - -0.1 217,436 + -0.4 217,437 - -0.2 217,437 - -0.4 217,437 - -0.1 217,458 - -1.3 217,599 + +0.2 217,599 + -2.1 217,599 + +0.8 217,815 + GFF907 0.32 -0.2 217,815 + GFF907 0.32 +0.1 217,816 - GFF907 0.32 -0.2 217,816 - GFF907 0.32 -0.6 217,816 - GFF907 0.32 -0.1 217,816 - GFF907 0.32 +0.5 217,816 - GFF907 0.32 -1.4 218,068 - GFF907 0.60 -0.1 218,068 - GFF907 0.60 -0.2
Or see this region's nucleotide sequence