Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF782 and GFF783 are separated by 101 nucleotides GFF783 and GFF784 are separated by 11 nucleotides GFF784 and GFF785 are separated by 5 nucleotides
GFF782 - Alpha-ketoglutarate-dependent dioxygenase AlkB (EC 1.14.11.33), at 64,538 to 65,245
GFF782
GFF783 - no description, at 65,347 to 66,069
GFF783
GFF784 - KEGG: RNA polymerase sigma-70 factor, ECF subfamily, at 66,081 to 66,641
GFF784
GFF785 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 66,647 to 67,636
GFF785
Position (kb) 66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 65.268 kb on + strand at 65.268 kb on + strand at 65.268 kb on + strand at 65.289 kb on + strand at 65.289 kb on + strand at 65.292 kb on + strand at 65.292 kb on + strand at 65.293 kb on - strand at 65.293 kb on - strand at 65.656 kb on + strand, within GFF783 at 66.132 kb on + strand at 66.132 kb on + strand at 66.132 kb on + strand at 66.132 kb on + strand at 66.133 kb on - strand at 66.261 kb on + strand, within GFF784 at 66.261 kb on + strand, within GFF784 at 66.631 kb on + strand at 66.631 kb on + strand at 66.631 kb on + strand at 66.632 kb on - strand at 66.634 kb on + strand at 66.789 kb on + strand, within GFF785 at 66.790 kb on - strand, within GFF785 at 66.917 kb on + strand, within GFF785 at 67.017 kb on - strand, within GFF785 at 67.171 kb on + strand, within GFF785 at 67.307 kb on + strand, within GFF785 at 67.352 kb on + strand, within GFF785 at 67.352 kb on + strand, within GFF785 at 67.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 65,268 + -0.1 65,268 + -0.3 65,268 + -2.2 65,289 + -0.1 65,289 + -1.2 65,292 + +1.3 65,292 + +1.5 65,293 - -0.1 65,293 - +0.5 65,656 + GFF783 0.43 -0.5 66,132 + -0.4 66,132 + -0.5 66,132 + -0.1 66,132 + -0.2 66,133 - -1.0 66,261 + GFF784 0.32 +1.0 66,261 + GFF784 0.32 -0.1 66,631 + +0.7 66,631 + -0.7 66,631 + +2.5 66,632 - -0.3 66,634 + +0.7 66,789 + GFF785 0.14 -0.1 66,790 - GFF785 0.14 -1.1 66,917 + GFF785 0.27 +0.8 67,017 - GFF785 0.37 -0.7 67,171 + GFF785 0.53 +0.8 67,307 + GFF785 0.67 -2.4 67,352 + GFF785 0.71 -0.1 67,352 + GFF785 0.71 -0.7 67,586 + -0.4
Or see this region's nucleotide sequence