Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF772 and GFF773 are separated by 61 nucleotides GFF773 and GFF774 are separated by 32 nucleotides GFF774 and GFF775 are separated by 99 nucleotides GFF775 and GFF776 are separated by 295 nucleotides
GFF772 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25) / Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 42,704 to 47,881
GFF772
GFF773 - hypothetical protein, at 47,943 to 48,269
GFF773
GFF774 - no description, at 48,302 to 48,508
GFF774
GFF775 - no description, at 48,608 to 48,874
GFF775
GFF776 - Putative large exoprotein involved in heme utilization or adhesion of ShlA/HecA/FhaA family, at 49,170 to 57,995
GFF776
Position (kb)
47
48
49 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 46.976 kb on + strand, within GFF772 at 46.986 kb on - strand, within GFF772 at 46.986 kb on - strand, within GFF772 at 46.986 kb on - strand, within GFF772 at 47.066 kb on + strand, within GFF772 at 47.066 kb on + strand, within GFF772 at 47.067 kb on - strand, within GFF772 at 47.067 kb on - strand, within GFF772 at 47.067 kb on - strand, within GFF772 at 47.067 kb on - strand, within GFF772 at 47.129 kb on + strand, within GFF772 at 47.130 kb on - strand, within GFF772 at 47.270 kb on + strand, within GFF772 at 47.270 kb on + strand, within GFF772 at 47.270 kb on + strand, within GFF772 at 47.271 kb on - strand, within GFF772 at 47.271 kb on - strand, within GFF772 at 47.298 kb on - strand, within GFF772 at 47.361 kb on - strand, within GFF772 at 47.411 kb on - strand at 47.854 kb on + strand at 47.854 kb on + strand at 47.855 kb on - strand at 47.876 kb on - strand at 47.876 kb on - strand at 47.876 kb on - strand at 47.876 kb on - strand at 48.054 kb on + strand, within GFF773 at 48.055 kb on - strand, within GFF773 at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.267 kb on + strand at 48.268 kb on - strand at 48.268 kb on - strand at 48.268 kb on - strand at 48.268 kb on - strand at 48.268 kb on - strand at 48.268 kb on - strand at 48.271 kb on + strand at 48.271 kb on + strand at 48.272 kb on - strand at 48.272 kb on - strand at 48.378 kb on + strand, within GFF774 at 48.379 kb on - strand, within GFF774 at 48.379 kb on - strand, within GFF774 at 48.379 kb on - strand, within GFF774 at 48.379 kb on - strand, within GFF774 at 48.379 kb on - strand, within GFF774 at 48.459 kb on + strand, within GFF774 at 48.459 kb on + strand, within GFF774 at 48.459 kb on + strand, within GFF774 at 48.460 kb on - strand, within GFF774 at 48.460 kb on - strand, within GFF774 at 48.460 kb on - strand, within GFF774 at 48.460 kb on - strand, within GFF774 at 48.460 kb on - strand, within GFF774 at 48.460 kb on - strand, within GFF774 at 48.460 kb on - strand, within GFF774 at 48.545 kb on - strand at 48.545 kb on - strand at 48.581 kb on + strand at 48.581 kb on + strand at 48.581 kb on + strand at 48.582 kb on - strand at 48.582 kb on - strand at 48.582 kb on - strand at 48.582 kb on - strand at 48.582 kb on - strand at 48.582 kb on - strand at 48.582 kb on - strand at 48.582 kb on - strand at 48.656 kb on + strand, within GFF775 at 48.657 kb on - strand, within GFF775 at 48.657 kb on - strand, within GFF775 at 48.657 kb on - strand, within GFF775 at 48.657 kb on - strand, within GFF775 at 48.657 kb on - strand, within GFF775 at 48.788 kb on + strand, within GFF775 at 48.926 kb on - strand at 48.929 kb on + strand at 48.929 kb on + strand at 48.930 kb on - strand at 48.957 kb on - strand at 48.957 kb on - strand at 48.963 kb on + strand at 48.963 kb on + strand at 48.963 kb on + strand at 48.963 kb on + strand at 48.964 kb on - strand at 48.964 kb on - strand at 48.964 kb on - strand at 48.964 kb on - strand at 48.967 kb on + strand at 48.967 kb on + strand at 48.967 kb on + strand at 48.967 kb on + strand at 48.968 kb on - strand at 48.968 kb on - strand at 48.968 kb on - strand at 48.968 kb on - strand at 48.968 kb on - strand at 48.968 kb on - strand at 48.968 kb on - strand at 48.970 kb on + strand at 48.971 kb on - strand at 49.025 kb on + strand at 49.025 kb on + strand at 49.025 kb on + strand at 49.025 kb on + strand at 49.025 kb on + strand at 49.026 kb on - strand at 49.026 kb on - strand at 49.026 kb on - strand at 49.046 kb on + strand at 49.047 kb on - strand at 49.098 kb on + strand at 49.098 kb on + strand at 49.099 kb on - strand at 49.099 kb on - strand at 49.114 kb on + strand at 49.240 kb on + strand at 49.240 kb on + strand at 49.241 kb on - strand at 49.241 kb on - strand at 49.241 kb on - strand at 49.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 46,976 + GFF772 0.83 +0.5 46,986 - GFF772 0.83 -0.3 46,986 - GFF772 0.83 -0.1 46,986 - GFF772 0.83 +0.3 47,066 + GFF772 0.84 -0.5 47,066 + GFF772 0.84 -0.3 47,067 - GFF772 0.84 -0.1 47,067 - GFF772 0.84 -0.8 47,067 - GFF772 0.84 -1.2 47,067 - GFF772 0.84 +0.6 47,129 + GFF772 0.85 -1.0 47,130 - GFF772 0.85 +0.0 47,270 + GFF772 0.88 +0.6 47,270 + GFF772 0.88 +1.1 47,270 + GFF772 0.88 +0.7 47,271 - GFF772 0.88 -0.2 47,271 - GFF772 0.88 +0.5 47,298 - GFF772 0.89 -0.2 47,361 - GFF772 0.90 -1.0 47,411 - -1.6 47,854 + -0.7 47,854 + +0.9 47,855 - -0.3 47,876 - +0.1 47,876 - -0.1 47,876 - +0.4 47,876 - -0.4 48,054 + GFF773 0.34 -1.4 48,055 - GFF773 0.34 -0.8 48,267 + -0.9 48,267 + -0.8 48,267 + +1.1 48,267 + -0.2 48,267 + -0.0 48,267 + -2.2 48,267 + -2.3 48,267 + -0.3 48,267 + +0.7 48,267 + -0.4 48,268 - -0.2 48,268 - -0.6 48,268 - -0.7 48,268 - -1.3 48,268 - -0.6 48,268 - -0.9 48,271 + +0.0 48,271 + -1.8 48,272 - -0.3 48,272 - -0.8 48,378 + GFF774 0.37 -1.1 48,379 - GFF774 0.37 -0.5 48,379 - GFF774 0.37 -0.2 48,379 - GFF774 0.37 -0.2 48,379 - GFF774 0.37 +0.0 48,379 - GFF774 0.37 -0.4 48,459 + GFF774 0.76 -0.5 48,459 + GFF774 0.76 +0.4 48,459 + GFF774 0.76 +0.5 48,460 - GFF774 0.76 -1.2 48,460 - GFF774 0.76 -0.0 48,460 - GFF774 0.76 -1.6 48,460 - GFF774 0.76 +0.1 48,460 - GFF774 0.76 -0.4 48,460 - GFF774 0.76 -1.5 48,460 - GFF774 0.76 -0.1 48,545 - -0.6 48,545 - -1.3 48,581 + +0.1 48,581 + -0.1 48,581 + -2.4 48,582 - +0.9 48,582 - +0.0 48,582 - +0.5 48,582 - +0.9 48,582 - +0.3 48,582 - +1.7 48,582 - -1.2 48,582 - -1.5 48,656 + GFF775 0.18 -0.5 48,657 - GFF775 0.18 -0.2 48,657 - GFF775 0.18 +0.5 48,657 - GFF775 0.18 -0.3 48,657 - GFF775 0.18 +3.1 48,657 - GFF775 0.18 -1.5 48,788 + GFF775 0.67 -0.6 48,926 - -0.4 48,929 + -1.1 48,929 + -1.5 48,930 - -0.2 48,957 - -0.2 48,957 - -1.6 48,963 + -0.4 48,963 + -1.3 48,963 + -0.3 48,963 + -0.6 48,964 - -0.4 48,964 - +1.5 48,964 - -0.1 48,964 - -0.1 48,967 + +0.5 48,967 + -0.2 48,967 + +0.8 48,967 + -0.1 48,968 - +0.2 48,968 - -0.5 48,968 - +1.5 48,968 - +1.1 48,968 - +0.5 48,968 - -1.5 48,968 - -0.6 48,970 + +0.3 48,971 - +0.4 49,025 + -0.8 49,025 + +0.4 49,025 + +1.1 49,025 + +0.7 49,025 + -0.1 49,026 - +0.0 49,026 - -0.1 49,026 - +0.5 49,046 + +1.0 49,047 - -0.7 49,098 + -1.6 49,098 + -0.7 49,099 - -0.4 49,099 - +0.4 49,114 + -0.8 49,240 + -1.1 49,240 + -0.3 49,241 - -1.2 49,241 - -0.2 49,241 - +0.0 49,241 - -0.6
Or see this region's nucleotide sequence