Strain Fitness in Variovorax sp. SCN45 around GFF7302

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
20,153	+		GFF7301	0.63	-0.3
20,154	-		GFF7301	0.63	-0.1
20,154	-		GFF7301	0.63	-0.3
20,195	+		GFF7301	0.65	-0.6
20,195	+		GFF7301	0.65	-0.1
20,196	-		GFF7301	0.65	+1.1
20,196	-		GFF7301	0.65	-0.0
20,196	-		GFF7301	0.65	-1.5
20,196	-		GFF7301	0.65	-0.2
20,247	-		GFF7301	0.67	-0.2
20,247	-		GFF7301	0.67	+0.2
20,247	-		GFF7301	0.67	-0.1
20,300	+		GFF7301	0.69	+0.1
20,300	+		GFF7301	0.69	-0.7
20,300	+		GFF7301	0.69	+0.3
20,300	+		GFF7301	0.69	+2.5
20,300	+		GFF7301	0.69	-1.1
20,300	+		GFF7301	0.69	-0.5
20,300	+		GFF7301	0.69	+0.4
20,300	+		GFF7301	0.69	-0.5
20,300	+		GFF7301	0.69	+0.4
20,300	+		GFF7301	0.69	-0.1
20,300	+		GFF7301	0.69	-0.7
20,300	+		GFF7301	0.69	-0.3
20,301	-		GFF7301	0.69	-1.2
20,301	-		GFF7301	0.69	-0.5
20,301	-		GFF7301	0.69	+0.3
20,301	-		GFF7301	0.69	+0.2
20,301	-		GFF7301	0.69	+0.9
20,301	-		GFF7301	0.69	-0.9
20,301	-		GFF7301	0.69	+1.1
20,301	-		GFF7301	0.69	+0.3
20,301	-		GFF7301	0.69	-1.1
20,301	-		GFF7301	0.69	-0.8
20,301	-		GFF7301	0.69	+0.3
20,301	-		GFF7301	0.69	+0.5
20,301	-		GFF7301	0.69	-1.1
20,301	-		GFF7301	0.69	-0.4
20,301	-		GFF7301	0.69	+0.1
20,387	+		GFF7301	0.72	+0.3
20,409	-		GFF7301	0.73	+0.5
20,409	-		GFF7301	0.73	-0.1
20,510	+		GFF7301	0.77	-0.5
20,510	+		GFF7301	0.77	+1.5
20,510	+		GFF7301	0.77	+0.4
20,561	+		GFF7301	0.79	+0.4
20,561	+		GFF7301	0.79	-1.7
20,562	-		GFF7301	0.79	-0.1
20,562	-		GFF7301	0.79	+0.3
20,615	+		GFF7301	0.81	-0.8
20,622	-		GFF7301	0.82	-0.8
20,622	-		GFF7301	0.82	-0.4
20,789	+		GFF7301	0.88	-2.5
20,789	+		GFF7301	0.88	+0.1
20,790	-		GFF7301	0.88	+0.3
20,790	-		GFF7301	0.88	+0.8
20,790	-		GFF7301	0.88	-0.3
20,790	-		GFF7301	0.88	-0.1
20,790	-		GFF7301	0.88	-1.2
20,790	-		GFF7301	0.88	-2.0
20,790	-		GFF7301	0.88	-0.1
20,790	-		GFF7301	0.88	+0.1
20,790	-		GFF7301	0.88	-0.7
20,852	+				-0.6
20,853	-				-0.7
20,853	-				-0.3
20,853	-				-0.0
20,853	-				-2.1
20,853	-				+0.9
20,853	-				-0.9
20,853	-				+0.7
20,855	+				-0.4
20,855	+				-0.5
20,855	+				-0.7
20,856	-				-0.3
20,856	-				+0.8
20,856	-				-0.1
20,856	-				-0.9
20,856	-				-1.1
20,856	-				-0.4
20,879	+				-0.4
20,879	+				+1.9
20,880	-				-0.9
20,880	-				+0.1
20,993	+				-0.7
20,993	+				-0.5
20,994	-				+0.1
21,157	+				-0.1
21,158	-				-0.5
21,238	+				+0.1
21,239	-				+0.4
21,239	-				-0.5
21,398	-		GFF7302	0.19	-0.9
21,398	-		GFF7302	0.19	-0.3
21,398	-		GFF7302	0.19	-0.0
21,398	-		GFF7302	0.19	-1.1
21,398	-		GFF7302	0.19	-0.5
21,409	+		GFF7302	0.20	+0.2
21,409	+		GFF7302	0.20	-0.9
21,409	+		GFF7302	0.20	-0.3
21,409	+		GFF7302	0.20	+1.0
21,409	+		GFF7302	0.20	-0.9
21,409	+		GFF7302	0.20	-0.5
21,410	-		GFF7302	0.20	-0.6
21,410	-		GFF7302	0.20	+0.3
21,410	-		GFF7302	0.20	-0.9
21,410	-		GFF7302	0.20	+0.2
21,410	-		GFF7302	0.20	+0.3
21,410	-		GFF7302	0.20	+0.4
21,778	+		GFF7302	0.45	-0.1
21,779	-		GFF7302	0.45	+0.1
21,779	-		GFF7302	0.45	-0.6
21,779	-		GFF7302	0.45	-1.5
21,779	-		GFF7302	0.45	-0.6
21,922	+		GFF7302	0.54	+1.3
21,922	+		GFF7302	0.54	-0.3
21,923	-		GFF7302	0.54	+0.1
21,923	-		GFF7302	0.54	+0.2
21,955	+		GFF7302	0.56	-0.4
21,956	-		GFF7302	0.56	-0.3
21,994	+		GFF7302	0.59	+1.9
21,994	+		GFF7302	0.59	-0.3
21,995	-		GFF7302	0.59	-0.3
21,995	-		GFF7302	0.59	-0.7
22,072	+		GFF7302	0.64	+1.6
22,073	-		GFF7302	0.64	-0.3
22,073	-		GFF7302	0.64	-2.5
22,150	+		GFF7302	0.69	+0.5
22,150	+		GFF7302	0.69	-0.5
22,150	+		GFF7302	0.69	+1.3
22,303	+		GFF7302	0.80	+0.3
22,303	+		GFF7302	0.80	-0.1
22,324	+		GFF7302	0.81	-0.5
22,325	-		GFF7302	0.81	+0.6
22,348	+		GFF7302	0.83	-0.7
22,348	+		GFF7302	0.83	-0.6
22,348	+		GFF7302	0.83	-0.2
22,348	+		GFF7302	0.83	+0.2
22,348	+		GFF7302	0.83	+0.3
22,348	+		GFF7302	0.83	-0.7
22,349	-		GFF7302	0.83	-0.0
22,349	-		GFF7302	0.83	+1.5
22,349	-		GFF7302	0.83	+0.6
22,349	-		GFF7302	0.83	-0.1
22,349	-		GFF7302	0.83	-1.0
22,611	+				+0.2
22,617	+				-0.7
22,617	+				-0.7
22,617	+				-0.7
22,617	+				-0.7
22,617	+				-1.4
22,617	+				+0.6
22,617	+				+0.3
22,617	+				+0.3
22,617	+				-1.5
22,617	+				-0.2
22,617	+				-1.1
22,617	+				+0.1
22,617	+				-0.7
22,618	-				-0.4
22,618	-				+0.0
22,618	-				-0.5
22,618	-				+0.1
22,618	-				-0.7
22,618	-				-0.1
22,618	-				-1.6
22,618	-				+0.0
22,618	-				-0.7
22,618	-				-0.6
22,618	-				+0.1
22,618	-				-0.7
22,618	-				-0.4
22,618	-				+0.8
22,988	+		GFF7303	0.27	+0.2
22,988	+		GFF7303	0.27	-0.1
22,988	+		GFF7303	0.27	-1.2
22,988	+		GFF7303	0.27	+0.3
22,988	+		GFF7303	0.27	-0.5
22,988	+		GFF7303	0.27	-0.3
22,989	-		GFF7303	0.28	-0.7
22,989	-		GFF7303	0.28	-0.2
22,989	-		GFF7303	0.28	-1.7
22,989	-		GFF7303	0.28	-0.6
23,015	+		GFF7303	0.30	-0.9
23,015	+		GFF7303	0.30	+0.7
23,015	+		GFF7303	0.30	-0.8
23,015	+		GFF7303	0.30	-0.0
23,015	+		GFF7303	0.30	+0.1
23,015	+		GFF7303	0.30	-1.5
23,016	-		GFF7303	0.30	-1.4
23,016	-		GFF7303	0.30	-0.2
23,016	-		GFF7303	0.30	+0.3
23,016	-		GFF7303	0.30	-0.7
23,016	-		GFF7303	0.30	-1.0
23,096	+		GFF7303	0.36	+0.2
23,096	+		GFF7303	0.36	-0.5
23,096	+		GFF7303	0.36	-0.2
23,096	+		GFF7303	0.36	-0.8
23,097	-		GFF7303	0.36	+0.3
23,097	-		GFF7303	0.36	-0.3
23,097	-		GFF7303	0.36	-0.5
23,097	-		GFF7303	0.36	+0.5
23,252	+		GFF7303	0.48	+0.5
23,252	+		GFF7303	0.48	+0.7
23,258	+		GFF7303	0.48	+0.0
23,259	-		GFF7303	0.48	-0.5
23,259	-		GFF7303	0.48	+0.6
23,259	-		GFF7303	0.48	-0.6
23,259	-		GFF7303	0.48	-0.7
23,276	+		GFF7303	0.49	-0.1
23,276	+		GFF7303	0.49	-1.5
23,276	+		GFF7303	0.49	+0.4
23,276	+		GFF7303	0.49	-0.7
23,276	+		GFF7303	0.49	+0.7
23,276	+		GFF7303	0.49	+0.5
23,276	+		GFF7303	0.49	-0.4
23,276	+		GFF7303	0.49	+1.9
23,277	-		GFF7303	0.49	+0.9
23,277	-		GFF7303	0.49	+0.1
23,277	-		GFF7303	0.49	+0.4
23,277	-		GFF7303	0.49	-0.0
23,277	-		GFF7303	0.49	+0.0
23,277	-		GFF7303	0.49	-0.0
23,277	-		GFF7303	0.49	-2.8
23,277	-		GFF7303	0.49	-1.0
23,277	-		GFF7303	0.49	-0.7
23,277	-		GFF7303	0.49	-0.0
23,277	-		GFF7303	0.49	-0.3
23,277	-		GFF7303	0.49	+0.1
23,277	-		GFF7303	0.49	-0.6
23,277	-		GFF7303	0.49	+0.4
23,477	+		GFF7303	0.65	-1.3
23,477	+		GFF7303	0.65	+0.7
23,478	-		GFF7303	0.65	-0.4
23,478	-		GFF7303	0.65	-1.7
23,478	-		GFF7303	0.65	+0.9
23,489	+		GFF7303	0.66	-0.3
23,489	+		GFF7303	0.66	+0.3
23,489	+		GFF7303	0.66	-0.1
23,489	+		GFF7303	0.66	-0.0
23,489	+		GFF7303	0.66	-0.7
23,489	+		GFF7303	0.66	-0.4
23,489	+		GFF7303	0.66	+0.3
23,489	+		GFF7303	0.66	-1.9
23,490	-		GFF7303	0.66	-0.3
23,490	-		GFF7303	0.66	-0.2
23,493	+		GFF7303	0.66	-1.3
23,493	+		GFF7303	0.66	-0.4
23,493	+		GFF7303	0.66	-1.1
23,493	+		GFF7303	0.66	+0.7
23,493	+		GFF7303	0.66	-0.6
23,493	+		GFF7303	0.66	-1.5
23,493	+		GFF7303	0.66	-0.9
23,493	+		GFF7303	0.66	+0.1
23,493	+		GFF7303	0.66	-0.2
23,493	+		GFF7303	0.66	+0.9
23,493	+		GFF7303	0.66	+1.3
23,493	+		GFF7303	0.66	+0.5
23,493	+		GFF7303	0.66	+0.5
23,493	+		GFF7303	0.66	-0.5
23,493	+		GFF7303	0.66	+0.3
23,493	+		GFF7303	0.66	+0.3
23,494	-		GFF7303	0.66	-0.6
23,494	-		GFF7303	0.66	-0.2
23,494	-		GFF7303	0.66	+1.9
23,494	-		GFF7303	0.66	-1.1
23,494	-		GFF7303	0.66	-0.5
23,494	-		GFF7303	0.66	-0.9
23,494	-		GFF7303	0.66	-0.3
23,494	-		GFF7303	0.66	+0.1
23,494	-		GFF7303	0.66	-0.8
23,494	-		GFF7303	0.66	+0.6
23,494	-		GFF7303	0.66	+0.5
23,494	-		GFF7303	0.66	+0.9
23,519	+		GFF7303	0.68	-0.8
23,519	+		GFF7303	0.68	-1.8
23,519	+		GFF7303	0.68	-1.0
23,519	+		GFF7303	0.68	-0.2
23,519	+		GFF7303	0.68	-1.9
23,519	+		GFF7303	0.68	-2.6
23,519	+		GFF7303	0.68	+0.1
23,520	-		GFF7303	0.68	-1.9
23,520	-		GFF7303	0.68	+1.1
23,520	-		GFF7303	0.68	+0.5
23,520	-		GFF7303	0.68	-2.0
23,589	-		GFF7303	0.73	-2.7