Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF72 and GFF73 are separated by 2 nucleotides GFF73 and GFF74 are separated by 72 nucleotides
GFF72 - D-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.-), at 89,244 to 90,377
GFF72
GFF73 - Probable haloacid dehalogenase, at 90,380 to 91,039
GFF73
GFF74 - Transcriptional regulator, LysR family, at 91,112 to 92,041
GFF74
Position (kb) 90
91
92 Strain fitness (log2 ratio) -2
-1
0
1 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 90.134 kb on - strand, within GFF72 at 90.495 kb on + strand, within GFF73 at 90.496 kb on - strand, within GFF73 at 90.496 kb on - strand, within GFF73 at 90.534 kb on + strand, within GFF73 at 90.535 kb on - strand, within GFF73 at 90.621 kb on + strand, within GFF73 at 90.621 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.889 kb on - strand, within GFF73 at 90.903 kb on + strand, within GFF73 at 91.052 kb on + strand at 91.052 kb on + strand at 91.052 kb on + strand at 91.053 kb on - strand at 91.053 kb on - strand at 91.099 kb on + strand at 91.428 kb on - strand, within GFF74 at 91.619 kb on + strand, within GFF74 at 91.620 kb on - strand, within GFF74 at 91.620 kb on - strand, within GFF74 at 91.799 kb on + strand, within GFF74 at 91.799 kb on + strand, within GFF74 at 91.799 kb on + strand, within GFF74 at 91.800 kb on - strand, within GFF74 at 91.800 kb on - strand, within GFF74 at 91.800 kb on - strand, within GFF74 at 91.800 kb on - strand, within GFF74 at 91.978 kb on + strand at 91.978 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 89,449 + GFF72 0.18 +0.4 89,449 + GFF72 0.18 +0.5 89,449 + GFF72 0.18 +1.1 89,449 + GFF72 0.18 -0.0 89,449 + GFF72 0.18 +0.3 89,449 + GFF72 0.18 -0.2 89,450 - GFF72 0.18 -0.6 89,450 - GFF72 0.18 -1.2 89,450 - GFF72 0.18 +0.5 89,671 + GFF72 0.38 +0.8 89,671 + GFF72 0.38 -0.6 89,671 + GFF72 0.38 -0.3 89,671 + GFF72 0.38 +0.5 89,671 + GFF72 0.38 -0.7 89,672 - GFF72 0.38 -0.6 89,672 - GFF72 0.38 -2.5 89,672 - GFF72 0.38 +0.2 90,134 - GFF72 0.78 -0.3 90,495 + GFF73 0.17 -0.5 90,496 - GFF73 0.18 +0.2 90,496 - GFF73 0.18 -0.7 90,534 + GFF73 0.23 +0.4 90,535 - GFF73 0.23 +0.4 90,621 + GFF73 0.37 +0.2 90,621 + GFF73 0.37 -1.9 90,873 + GFF73 0.75 -0.4 90,873 + GFF73 0.75 +1.5 90,873 + GFF73 0.75 +0.2 90,873 + GFF73 0.75 +0.2 90,873 + GFF73 0.75 +0.0 90,874 - GFF73 0.75 -2.1 90,874 - GFF73 0.75 -1.0 90,874 - GFF73 0.75 +0.3 90,874 - GFF73 0.75 +0.5 90,889 - GFF73 0.77 -0.2 90,903 + GFF73 0.79 -0.2 91,052 + -0.3 91,052 + -0.7 91,052 + -2.0 91,053 - -1.1 91,053 - +1.7 91,099 + -0.5 91,428 - GFF74 0.34 +0.4 91,619 + GFF74 0.55 +1.0 91,620 - GFF74 0.55 -0.1 91,620 - GFF74 0.55 -1.6 91,799 + GFF74 0.74 +0.4 91,799 + GFF74 0.74 -2.1 91,799 + GFF74 0.74 -0.1 91,800 - GFF74 0.74 -0.4 91,800 - GFF74 0.74 +0.4 91,800 - GFF74 0.74 -0.2 91,800 - GFF74 0.74 -1.7 91,978 + -0.8 91,978 + -0.1
Or see this region's nucleotide sequence