Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7188 and GFF7189 are separated by 498 nucleotides GFF7189 and GFF7190 are separated by 57 nucleotides
GFF7188 - Uncharacterized methyltransferase PA1407, at 4,676 to 5,611
GFF7188
GFF7189 - dioxygenase, TauD/TfdA family, at 6,110 to 7,027
GFF7189
GFF7190 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 7,085 to 8,455
GFF7190
Position (kb) 6
7
8 Strain fitness (log2 ratio) -1
0
1
2 at 5.149 kb on - strand, within GFF7188 at 5.149 kb on - strand, within GFF7188 at 5.611 kb on + strand at 5.611 kb on + strand at 5.612 kb on - strand at 5.612 kb on - strand at 5.617 kb on + strand at 5.617 kb on + strand at 5.617 kb on + strand at 5.617 kb on + strand at 5.838 kb on + strand at 5.917 kb on + strand at 6.023 kb on + strand at 6.023 kb on + strand at 6.024 kb on - strand at 6.350 kb on + strand, within GFF7189 at 6.651 kb on - strand, within GFF7189 at 6.836 kb on + strand, within GFF7189 at 6.893 kb on + strand, within GFF7189 at 6.893 kb on + strand, within GFF7189 at 6.894 kb on - strand, within GFF7189 at 6.895 kb on + strand, within GFF7189 at 6.895 kb on + strand, within GFF7189 at 7.455 kb on + strand, within GFF7190 at 7.455 kb on + strand, within GFF7190 at 7.456 kb on - strand, within GFF7190 at 7.595 kb on + strand, within GFF7190 at 7.596 kb on - strand, within GFF7190 at 7.658 kb on + strand, within GFF7190 at 7.658 kb on + strand, within GFF7190 at 7.659 kb on - strand, within GFF7190 at 7.659 kb on - strand, within GFF7190 at 7.703 kb on + strand, within GFF7190 at 7.704 kb on - strand, within GFF7190 at 7.704 kb on - strand, within GFF7190 at 7.976 kb on + strand, within GFF7190
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 5,149 - GFF7188 0.51 -0.8 5,149 - GFF7188 0.51 -0.7 5,611 + +2.0 5,611 + -0.6 5,612 - +0.6 5,612 - +0.4 5,617 + +1.2 5,617 + -0.3 5,617 + -0.8 5,617 + +0.1 5,838 + -0.1 5,917 + -1.5 6,023 + -0.6 6,023 + +2.7 6,024 - +0.0 6,350 + GFF7189 0.26 -1.0 6,651 - GFF7189 0.59 -0.6 6,836 + GFF7189 0.79 -0.5 6,893 + GFF7189 0.85 -1.4 6,893 + GFF7189 0.85 +0.2 6,894 - GFF7189 0.85 -1.7 6,895 + GFF7189 0.86 -1.1 6,895 + GFF7189 0.86 -0.4 7,455 + GFF7190 0.27 -1.4 7,455 + GFF7190 0.27 -0.2 7,456 - GFF7190 0.27 -0.5 7,595 + GFF7190 0.37 +0.6 7,596 - GFF7190 0.37 -0.3 7,658 + GFF7190 0.42 +1.2 7,658 + GFF7190 0.42 -0.1 7,659 - GFF7190 0.42 -0.6 7,659 - GFF7190 0.42 +1.3 7,703 + GFF7190 0.45 +0.8 7,704 - GFF7190 0.45 -0.2 7,704 - GFF7190 0.45 -0.6 7,976 + GFF7190 0.65 -0.4
Or see this region's nucleotide sequence