Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7146 and GFF7147 overlap by 4 nucleotides GFF7147 and GFF7148 overlap by 4 nucleotides GFF7148 and GFF7149 are separated by 11 nucleotides GFF7149 and GFF7150 are separated by 14 nucleotides
GFF7146 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 12,084 to 13,388
GFF7146
GFF7147 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 13,385 to 13,639
GFF7147
GFF7148 - D-amino-acid oxidase (EC 1.4.3.3), at 13,636 to 14,739
GFF7148
GFF7149 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 14,751 to 15,686
GFF7149
GFF7150 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 15,701 to 16,843
GFF7150
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.886 kb on + strand, within GFF7148 at 13.886 kb on + strand, within GFF7148 at 13.887 kb on - strand, within GFF7148 at 13.887 kb on - strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.049 kb on - strand, within GFF7148 at 14.049 kb on - strand, within GFF7148 at 14.049 kb on - strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.504 kb on + strand, within GFF7148 at 14.504 kb on + strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.866 kb on + strand, within GFF7149 at 14.866 kb on + strand, within GFF7149 at 14.866 kb on + strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.992 kb on + strand, within GFF7149 at 14.993 kb on - strand, within GFF7149 at 14.993 kb on - strand, within GFF7149 at 14.993 kb on - strand, within GFF7149 at 15.133 kb on + strand, within GFF7149 at 15.133 kb on + strand, within GFF7149 at 15.134 kb on - strand, within GFF7149 at 15.134 kb on - strand, within GFF7149 at 15.134 kb on - strand, within GFF7149 at 15.277 kb on + strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 13,886 + GFF7148 0.23 -1.2 13,886 + GFF7148 0.23 -0.4 13,887 - GFF7148 0.23 +0.6 13,887 - GFF7148 0.23 +1.0 14,048 + GFF7148 0.37 -0.6 14,048 + GFF7148 0.37 -1.0 14,048 + GFF7148 0.37 -0.2 14,048 + GFF7148 0.37 +0.7 14,048 + GFF7148 0.37 +0.8 14,049 - GFF7148 0.37 +1.4 14,049 - GFF7148 0.37 +0.3 14,049 - GFF7148 0.37 -0.6 14,312 + GFF7148 0.61 -0.0 14,312 + GFF7148 0.61 +0.1 14,312 + GFF7148 0.61 -0.3 14,312 + GFF7148 0.61 -1.7 14,312 + GFF7148 0.61 +2.4 14,312 + GFF7148 0.61 +0.1 14,312 + GFF7148 0.61 -1.1 14,312 + GFF7148 0.61 +0.3 14,312 + GFF7148 0.61 +0.1 14,312 + GFF7148 0.61 -0.6 14,313 - GFF7148 0.61 -2.1 14,313 - GFF7148 0.61 +1.0 14,313 - GFF7148 0.61 +0.3 14,313 - GFF7148 0.61 -0.9 14,504 + GFF7148 0.79 -0.2 14,504 + GFF7148 0.79 +0.6 14,505 - GFF7148 0.79 +0.2 14,505 - GFF7148 0.79 -0.8 14,505 - GFF7148 0.79 -0.7 14,505 - GFF7148 0.79 -0.5 14,505 - GFF7148 0.79 -0.1 14,866 + GFF7149 0.12 -0.6 14,866 + GFF7149 0.12 -1.1 14,866 + GFF7149 0.12 -1.6 14,867 - GFF7149 0.12 -1.7 14,867 - GFF7149 0.12 +0.7 14,867 - GFF7149 0.12 -0.6 14,867 - GFF7149 0.12 +0.1 14,992 + GFF7149 0.26 -0.5 14,993 - GFF7149 0.26 -0.7 14,993 - GFF7149 0.26 +0.7 14,993 - GFF7149 0.26 -0.3 15,133 + GFF7149 0.41 -1.7 15,133 + GFF7149 0.41 +0.9 15,134 - GFF7149 0.41 +0.8 15,134 - GFF7149 0.41 -0.0 15,134 - GFF7149 0.41 -1.2 15,277 + GFF7149 0.56 -1.0 15,437 - GFF7149 0.73 -0.1 15,437 - GFF7149 0.73 +1.0 15,437 - GFF7149 0.73 -0.9 15,437 - GFF7149 0.73 -0.5 15,705 + -2.6 15,705 + +0.7 15,705 + +0.7 15,705 + -0.6 15,705 + +0.1 15,705 + -0.5 15,705 + -1.9 15,705 + -0.3 15,705 + +0.0 15,705 + -0.0 15,706 - -0.3 15,706 - +0.2 15,706 - -1.0 15,706 - -1.2 15,706 - -0.8 15,706 - +0.2 15,706 - -0.7 15,706 - +0.7 15,706 - -0.5 15,706 - -0.7 15,706 - -0.2 15,706 - -0.6 15,706 - +0.4
Or see this region's nucleotide sequence