Strain Fitness in Variovorax sp. SCN45 around GFF6533

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
28,620	-		GFF6532	0.67	-0.5
28,620	-		GFF6532	0.67	-1.5
28,620	-		GFF6532	0.67	+0.4
28,620	-		GFF6532	0.67	+0.2
28,719	-		GFF6532	0.74	+0.2
28,719	-		GFF6532	0.74	+0.3
28,719	-		GFF6532	0.74	-0.1
28,758	-		GFF6532	0.77	-0.9
28,758	-		GFF6532	0.77	-0.4
28,758	-		GFF6532	0.77	-0.1
28,758	-		GFF6532	0.77	-0.9
28,824	-		GFF6532	0.82	+0.5
28,824	-		GFF6532	0.82	-0.4
28,824	-		GFF6532	0.82	+0.4
29,046	-				+0.2
29,046	-				-0.5
29,046	-				+0.7
29,046	-				-1.3
29,103	-				-0.4
29,116	+				-1.1
29,116	+				-0.8
29,116	+				-0.8
29,116	+				-0.2
29,117	-				-0.6
29,117	-				+0.4
29,117	-				-1.3
29,117	-				-1.9
29,176	+				-0.2
29,193	+				-2.5
29,193	+				+0.4
29,193	+				+0.1
29,194	-				-0.2
29,236	+				-1.8
29,236	+				+0.6
29,236	+				-0.1
29,236	+				+0.4
29,236	+				-0.1
29,236	+				-0.2
29,237	-				-0.5
29,237	-				-1.1
29,237	-				-1.1
29,237	-				-0.1
29,308	+				-0.2
29,308	+				+0.0
29,308	+				+0.5
29,308	+				-0.8
29,308	+				-0.6
29,308	+				-0.5
29,308	+				+0.5
29,308	+				-0.7
29,308	+				-0.3
29,309	-				-1.9
29,309	-				+1.0
29,309	-				+0.2
29,309	-				-0.3
29,309	-				+0.7
29,309	-				-0.5
29,309	-				-1.1
29,309	-				-0.5
29,309	-				-0.2
29,623	+		GFF6533	0.27	-0.8
29,623	+		GFF6533	0.27	-1.1
29,623	+		GFF6533	0.27	-0.4
29,623	+		GFF6533	0.27	-1.1
29,623	+		GFF6533	0.27	+0.5
29,624	-		GFF6533	0.27	-0.2
29,624	-		GFF6533	0.27	-1.4
29,752	+		GFF6533	0.36	+0.5
29,753	-		GFF6533	0.36	-0.5
29,753	-		GFF6533	0.36	-0.5
29,850	+		GFF6533	0.42	-0.5
29,851	+		GFF6533	0.42	+0.2
29,851	+		GFF6533	0.42	-0.5
29,852	-		GFF6533	0.42	-2.1
29,852	-		GFF6533	0.42	+0.7
29,909	+		GFF6533	0.45	-0.1
29,909	+		GFF6533	0.45	+0.2
29,910	-		GFF6533	0.45	-0.8
29,959	+		GFF6533	0.49	-0.4
29,959	+		GFF6533	0.49	-0.7
29,959	+		GFF6533	0.49	-0.5
29,960	-		GFF6533	0.49	-0.4
29,960	-		GFF6533	0.49	-1.6
29,960	-		GFF6533	0.49	-1.5
30,153	+		GFF6533	0.61	+1.2
30,154	-		GFF6533	0.61	-0.9
30,154	-		GFF6533	0.61	-0.2
30,547	+		GFF6533	0.86	+0.7
30,547	+		GFF6533	0.86	-0.3
30,547	+		GFF6533	0.86	-2.2
30,547	+		GFF6533	0.86	-1.0
30,548	-		GFF6533	0.86	-1.7
30,548	-		GFF6533	0.86	-1.5
30,548	-		GFF6533	0.86	-0.1
30,548	-		GFF6533	0.86	+0.1
30,679	+				-1.1
30,679	+				-1.1
30,680	-				-0.4
30,680	-				-0.7
30,680	-				+0.4
30,680	-				-0.8
30,680	-				+0.2
30,805	+				-1.2
30,805	+				-0.5
30,805	+				+0.9
30,805	+				+0.7
30,805	+				-1.3
30,805	+				-1.0
30,805	+				+0.1
30,805	+				-0.5
30,805	+				-0.9
30,806	-				+0.1
30,806	-				-0.2
30,806	-				-0.6
30,806	-				+0.3
30,806	-				-0.3
31,090	+		GFF6534	0.62	-1.8
31,090	+		GFF6534	0.62	-0.5
31,090	+		GFF6534	0.62	+0.3
31,090	+		GFF6534	0.62	-1.0
31,091	-		GFF6534	0.62	-1.3
31,091	-		GFF6534	0.62	-0.7
31,277	-				+0.7
31,277	-				-0.0