Strain Fitness in Variovorax sp. SCN45 around GFF6525

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
20,805	+		GFF6524	0.61	+0.5
20,806	-		GFF6524	0.61	-0.6
20,806	-		GFF6524	0.61	-0.3
20,806	-		GFF6524	0.61	-0.4
20,806	-		GFF6524	0.61	-0.2
20,826	+		GFF6524	0.62	-0.5
20,889	+		GFF6524	0.65	-0.1
20,889	+		GFF6524	0.65	+0.8
20,889	+		GFF6524	0.65	-0.6
20,890	-		GFF6524	0.65	-1.1
20,890	-		GFF6524	0.65	-1.2
20,890	-		GFF6524	0.65	-0.7
20,931	+		GFF6524	0.67	+0.0
20,931	+		GFF6524	0.67	-0.1
20,931	+		GFF6524	0.67	+0.9
20,931	+		GFF6524	0.67	-0.2
20,931	+		GFF6524	0.67	+0.5
20,932	-		GFF6524	0.67	-0.1
20,932	-		GFF6524	0.67	-0.3
20,932	-		GFF6524	0.67	-0.4
20,932	-		GFF6524	0.67	-0.5
20,934	+		GFF6524	0.67	-0.8
20,934	+		GFF6524	0.67	-0.0
20,934	+		GFF6524	0.67	+0.8
20,934	+		GFF6524	0.67	+0.4
20,935	-		GFF6524	0.67	-1.3
20,935	-		GFF6524	0.67	-1.2
20,935	-		GFF6524	0.67	-1.9
20,935	-		GFF6524	0.67	-0.3
20,935	-		GFF6524	0.67	-0.2
21,045	+		GFF6524	0.72	-0.0
21,045	+		GFF6524	0.72	+1.0
21,045	+		GFF6524	0.72	-0.8
21,045	+		GFF6524	0.72	-0.3
21,045	+		GFF6524	0.72	-1.2
21,045	+		GFF6524	0.72	+0.8
21,046	-		GFF6524	0.72	+0.0
21,046	-		GFF6524	0.72	-0.5
21,046	-		GFF6524	0.72	+0.1
21,046	-		GFF6524	0.72	-0.3
21,046	-		GFF6524	0.72	-0.1
21,046	-		GFF6524	0.72	+0.4
21,048	+		GFF6524	0.72	-0.6
21,048	+		GFF6524	0.72	-1.2
21,048	+		GFF6524	0.72	-2.1
21,048	+		GFF6524	0.72	-0.5
21,048	+		GFF6524	0.72	+0.3
21,048	+		GFF6524	0.72	+0.5
21,048	+		GFF6524	0.72	-2.6
21,048	+		GFF6524	0.72	-0.6
21,048	+		GFF6524	0.72	+0.2
21,048	+		GFF6524	0.72	-0.3
21,048	+		GFF6524	0.72	-0.6
21,049	-		GFF6524	0.72	-1.0
21,049	-		GFF6524	0.72	+0.0
21,049	-		GFF6524	0.72	-2.5
21,049	-		GFF6524	0.72	+0.2
21,049	-		GFF6524	0.72	+0.5
21,049	-		GFF6524	0.72	+0.1
21,049	-		GFF6524	0.72	-0.5
21,049	-		GFF6524	0.72	-0.3
21,192	+		GFF6524	0.79	-1.1
21,192	+		GFF6524	0.79	+0.5
21,237	+		GFF6524	0.81	-0.5
21,238	-		GFF6524	0.81	-0.1
21,238	-		GFF6524	0.81	-0.1
21,238	-		GFF6524	0.81	-0.2
21,238	-		GFF6524	0.81	+2.0
21,238	-		GFF6524	0.81	-0.0
21,304	-		GFF6524	0.84	-0.6
21,450	+				+0.0
21,718	-				-1.8
21,810	-		GFF6525	0.18	-1.0
21,893	+		GFF6525	0.39	-1.4
21,893	+		GFF6525	0.39	+0.2
21,893	+		GFF6525	0.39	+0.5
21,893	+		GFF6525	0.39	-0.2
21,893	+		GFF6525	0.39	-0.6
21,894	-		GFF6525	0.40	-1.1
21,894	-		GFF6525	0.40	-0.9
21,894	-		GFF6525	0.40	-0.3
22,213	+		GFF6526	0.13	+0.3
22,213	+		GFF6526	0.13	-0.2
22,214	-		GFF6526	0.13	-0.2
22,214	-		GFF6526	0.13	-1.4
22,240	+		GFF6526	0.18	+0.1
22,240	+		GFF6526	0.18	-0.4
22,240	+		GFF6526	0.18	-0.0
22,241	-		GFF6526	0.18	-1.3
22,241	-		GFF6526	0.18	-1.7
22,241	-		GFF6526	0.18	-0.9
22,241	-		GFF6526	0.18	+0.0
22,241	-		GFF6526	0.18	-1.0
22,252	+		GFF6526	0.20	+0.3
22,252	+		GFF6526	0.20	+0.2
22,253	-		GFF6526	0.20	-0.4
22,253	-		GFF6526	0.20	+0.1
22,253	-		GFF6526	0.20	+0.1
22,253	-		GFF6526	0.20	-1.3
22,270	+		GFF6526	0.23	-0.3
22,270	+		GFF6526	0.23	+0.0
22,270	+		GFF6526	0.23	+0.8
22,271	-		GFF6526	0.23	-0.6
22,271	-		GFF6526	0.23	+3.8
22,271	-		GFF6526	0.23	-1.4
22,271	-		GFF6526	0.23	+0.3
22,312	+		GFF6526	0.31	+0.4
22,312	+		GFF6526	0.31	+0.5
22,312	+		GFF6526	0.31	+1.1
22,312	+		GFF6526	0.31	-0.7
22,312	+		GFF6526	0.31	-0.7
22,313	-		GFF6526	0.31	+0.4
22,313	-		GFF6526	0.31	+0.1
22,313	-		GFF6526	0.31	-0.4
22,313	-		GFF6526	0.31	-1.6
22,313	-		GFF6526	0.31	-1.8
22,378	+		GFF6526	0.43	+0.5
22,378	+		GFF6526	0.43	+1.3
22,379	-		GFF6526	0.43	+0.3
22,379	-		GFF6526	0.43	-0.7
22,379	-		GFF6526	0.43	+0.2
22,379	-		GFF6526	0.43	-0.3
22,379	-		GFF6526	0.43	+0.1
22,381	+		GFF6526	0.43	+1.0
22,381	+		GFF6526	0.43	+1.1
22,381	+		GFF6526	0.43	-1.3
22,381	+		GFF6526	0.43	-0.5
22,381	+		GFF6526	0.43	-1.6
22,381	+		GFF6526	0.43	+0.5
22,381	+		GFF6526	0.43	-0.1
22,381	+		GFF6526	0.43	+0.2
22,381	+		GFF6526	0.43	-1.9
22,381	+		GFF6526	0.43	+0.5
22,382	-		GFF6526	0.43	+0.3
22,382	-		GFF6526	0.43	+0.9
22,382	-		GFF6526	0.43	-0.6
22,382	-		GFF6526	0.43	-0.7
22,382	-		GFF6526	0.43	-1.2
22,382	-		GFF6526	0.43	-2.1
22,382	-		GFF6526	0.43	-0.7
22,382	-		GFF6526	0.43	-1.8
22,396	+		GFF6526	0.46	-0.1
22,397	-		GFF6526	0.46	-0.3
22,552	+		GFF6526	0.74	+0.5
22,552	+		GFF6526	0.74	+1.0
22,553	-		GFF6526	0.74	+0.2
22,553	-		GFF6526	0.74	-0.6
22,553	-		GFF6526	0.74	+1.0
22,582	+		GFF6526	0.80	-0.1
22,768	-				+0.8
22,772	+				+0.3
22,772	+				+2.0
22,773	-				+0.7
22,790	+				-0.2
22,986	+		GFF6527	0.28	-0.5
22,986	+		GFF6527	0.28	-0.2
22,986	+		GFF6527	0.28	-0.1
22,987	-		GFF6527	0.28	-0.4
22,987	-		GFF6527	0.28	-1.9
22,987	-		GFF6527	0.28	-0.2
22,987	-		GFF6527	0.28	-0.0
23,049	-		GFF6527	0.37	+0.4
23,106	+		GFF6527	0.46	-0.4
23,106	+		GFF6527	0.46	+0.4
23,107	-		GFF6527	0.46	-2.7