Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6493 and GFF6494 are separated by 83 nucleotides GFF6494 and GFF6495 are separated by 93 nucleotides GFF6495 and GFF6496 are separated by 2 nucleotides GFF6496 and GFF6497 are separated by 183 nucleotides
GFF6493 - Ribosomal-protein-S5p-alanine acetyltransferase, at 70,196 to 70,549
GFF6493
GFF6494 - protein of unknown function DUF1232, at 70,633 to 71,025
GFF6494
GFF6495 - rifampin ADP-ribosyl transferase, at 71,119 to 71,514
GFF6495
GFF6496 - Tannase and feruloyl esterase, at 71,517 to 73,235
GFF6496
GFF6497 - Protein of unknown function (DUF1501), at 73,419 to 74,630
GFF6497
Position (kb) 71
72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 70.547 kb on + strand at 70.548 kb on - strand at 70.548 kb on - strand at 70.605 kb on + strand at 70.605 kb on + strand at 70.605 kb on + strand at 70.605 kb on + strand at 70.605 kb on + strand at 70.605 kb on + strand at 70.605 kb on + strand at 70.605 kb on + strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.606 kb on - strand at 70.719 kb on + strand, within GFF6494 at 70.719 kb on + strand, within GFF6494 at 70.719 kb on + strand, within GFF6494 at 70.720 kb on - strand, within GFF6494 at 70.729 kb on + strand, within GFF6494 at 70.762 kb on + strand, within GFF6494 at 70.762 kb on - strand, within GFF6494 at 70.763 kb on - strand, within GFF6494 at 70.813 kb on + strand, within GFF6494 at 70.814 kb on - strand, within GFF6494 at 70.829 kb on + strand, within GFF6494 at 70.829 kb on + strand, within GFF6494 at 70.829 kb on + strand, within GFF6494 at 70.829 kb on + strand, within GFF6494 at 70.829 kb on + strand, within GFF6494 at 70.829 kb on + strand, within GFF6494 at 70.829 kb on - strand, within GFF6494 at 70.830 kb on - strand, within GFF6494 at 70.830 kb on - strand, within GFF6494 at 70.830 kb on - strand, within GFF6494 at 71.143 kb on + strand at 71.143 kb on + strand at 71.143 kb on + strand at 71.143 kb on + strand at 71.143 kb on + strand at 71.143 kb on + strand at 71.144 kb on - strand at 71.144 kb on - strand at 71.144 kb on - strand at 71.209 kb on + strand, within GFF6495 at 71.209 kb on + strand, within GFF6495 at 71.239 kb on + strand, within GFF6495 at 71.239 kb on + strand, within GFF6495 at 71.239 kb on + strand, within GFF6495 at 71.239 kb on + strand, within GFF6495 at 71.239 kb on + strand, within GFF6495 at 71.239 kb on + strand, within GFF6495 at 71.239 kb on + strand, within GFF6495 at 71.240 kb on - strand, within GFF6495 at 71.240 kb on - strand, within GFF6495 at 71.240 kb on - strand, within GFF6495 at 71.240 kb on - strand, within GFF6495 at 71.240 kb on - strand, within GFF6495 at 71.240 kb on - strand, within GFF6495 at 71.240 kb on - strand, within GFF6495 at 71.311 kb on + strand, within GFF6495 at 71.311 kb on + strand, within GFF6495 at 71.311 kb on + strand, within GFF6495 at 71.311 kb on + strand, within GFF6495 at 71.311 kb on + strand, within GFF6495 at 71.312 kb on - strand, within GFF6495 at 71.312 kb on - strand, within GFF6495 at 71.326 kb on - strand, within GFF6495 at 71.392 kb on + strand, within GFF6495 at 71.392 kb on + strand, within GFF6495 at 71.393 kb on - strand, within GFF6495 at 71.458 kb on + strand, within GFF6495 at 71.458 kb on + strand, within GFF6495 at 71.458 kb on + strand, within GFF6495 at 71.458 kb on + strand, within GFF6495 at 71.458 kb on + strand, within GFF6495 at 71.459 kb on - strand, within GFF6495 at 71.459 kb on - strand, within GFF6495 at 71.459 kb on - strand, within GFF6495 at 71.459 kb on - strand, within GFF6495 at 71.459 kb on - strand, within GFF6495 at 71.459 kb on - strand, within GFF6495 at 71.542 kb on + strand at 71.543 kb on - strand at 71.575 kb on + strand at 71.575 kb on + strand at 71.575 kb on + strand at 71.575 kb on + strand at 71.575 kb on + strand at 71.575 kb on + strand at 71.576 kb on - strand at 71.585 kb on - strand at 71.585 kb on - strand at 71.763 kb on - strand, within GFF6496 at 71.791 kb on + strand, within GFF6496 at 71.791 kb on + strand, within GFF6496 at 71.791 kb on + strand, within GFF6496 at 71.791 kb on + strand, within GFF6496 at 71.792 kb on - strand, within GFF6496 at 71.792 kb on - strand, within GFF6496 at 71.848 kb on + strand, within GFF6496 at 71.848 kb on + strand, within GFF6496 at 71.849 kb on - strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 71.851 kb on + strand, within GFF6496 at 72.046 kb on + strand, within GFF6496 at 72.047 kb on - strand, within GFF6496 at 72.055 kb on + strand, within GFF6496 at 72.055 kb on + strand, within GFF6496 at 72.055 kb on + strand, within GFF6496 at 72.055 kb on + strand, within GFF6496 at 72.055 kb on + strand, within GFF6496 at 72.056 kb on - strand, within GFF6496 at 72.056 kb on - strand, within GFF6496 at 72.056 kb on - strand, within GFF6496 at 72.056 kb on - strand, within GFF6496 at 72.056 kb on - strand, within GFF6496 at 72.056 kb on - strand, within GFF6496 at 72.067 kb on + strand, within GFF6496 at 72.067 kb on + strand, within GFF6496 at 72.068 kb on - strand, within GFF6496 at 72.068 kb on - strand, within GFF6496 at 72.172 kb on + strand, within GFF6496 at 72.172 kb on + strand, within GFF6496 at 72.236 kb on - strand, within GFF6496 at 72.346 kb on + strand, within GFF6496 at 72.346 kb on + strand, within GFF6496 at 72.347 kb on - strand, within GFF6496 at 72.347 kb on - strand, within GFF6496 at 72.347 kb on - strand, within GFF6496 at 72.347 kb on - strand, within GFF6496 at 72.347 kb on - strand, within GFF6496 at 72.347 kb on - strand, within GFF6496 at 72.466 kb on + strand, within GFF6496 at 72.466 kb on + strand, within GFF6496 at 72.467 kb on - strand, within GFF6496 at 72.467 kb on - strand, within GFF6496 at 72.467 kb on - strand, within GFF6496 at 72.592 kb on + strand, within GFF6496 at 72.592 kb on + strand, within GFF6496 at 72.592 kb on + strand, within GFF6496 at 72.592 kb on + strand, within GFF6496 at 72.592 kb on + strand, within GFF6496 at 72.592 kb on + strand, within GFF6496 at 72.592 kb on + strand, within GFF6496 at 72.593 kb on - strand, within GFF6496 at 72.593 kb on - strand, within GFF6496 at 72.593 kb on - strand, within GFF6496 at 72.593 kb on - strand, within GFF6496 at 72.593 kb on - strand, within GFF6496 at 72.598 kb on + strand, within GFF6496 at 72.598 kb on + strand, within GFF6496 at 72.598 kb on + strand, within GFF6496 at 72.598 kb on + strand, within GFF6496 at 72.598 kb on + strand, within GFF6496 at 72.599 kb on - strand, within GFF6496 at 72.599 kb on - strand, within GFF6496 at 72.599 kb on - strand, within GFF6496 at 72.599 kb on - strand, within GFF6496 at 72.599 kb on - strand, within GFF6496 at 72.599 kb on - strand, within GFF6496 at 72.599 kb on - strand, within GFF6496 at 72.622 kb on + strand, within GFF6496 at 72.622 kb on + strand, within GFF6496 at 72.622 kb on + strand, within GFF6496 at 72.622 kb on + strand, within GFF6496 at 72.622 kb on + strand, within GFF6496 at 72.623 kb on - strand, within GFF6496 at 72.623 kb on - strand, within GFF6496 at 72.625 kb on + strand, within GFF6496 at 72.625 kb on + strand, within GFF6496 at 72.625 kb on + strand, within GFF6496 at 72.625 kb on + strand, within GFF6496 at 72.626 kb on - strand, within GFF6496 at 72.626 kb on - strand, within GFF6496 at 72.626 kb on - strand, within GFF6496 at 72.626 kb on - strand, within GFF6496 at 72.626 kb on - strand, within GFF6496 at 72.626 kb on - strand, within GFF6496 at 72.667 kb on + strand, within GFF6496 at 72.667 kb on + strand, within GFF6496 at 72.668 kb on - strand, within GFF6496 at 72.668 kb on - strand, within GFF6496 at 72.668 kb on - strand, within GFF6496 at 72.668 kb on - strand, within GFF6496 at 72.668 kb on - strand, within GFF6496 at 72.737 kb on - strand, within GFF6496 at 72.737 kb on - strand, within GFF6496 at 72.737 kb on - strand, within GFF6496 at 72.737 kb on - strand, within GFF6496 at 72.921 kb on + strand, within GFF6496 at 72.921 kb on + strand, within GFF6496 at 72.922 kb on - strand, within GFF6496 at 72.922 kb on - strand, within GFF6496 at 72.973 kb on + strand, within GFF6496 at 72.973 kb on + strand, within GFF6496 at 72.973 kb on + strand, within GFF6496 at 72.974 kb on - strand, within GFF6496 at 72.995 kb on + strand, within GFF6496 at 72.996 kb on - strand, within GFF6496 at 72.999 kb on + strand, within GFF6496 at 72.999 kb on + strand, within GFF6496 at 73.008 kb on + strand, within GFF6496 at 73.008 kb on + strand, within GFF6496 at 73.008 kb on + strand, within GFF6496 at 73.008 kb on + strand, within GFF6496 at 73.008 kb on + strand, within GFF6496 at 73.009 kb on - strand, within GFF6496 at 73.009 kb on - strand, within GFF6496 at 73.009 kb on - strand, within GFF6496 at 73.263 kb on + strand at 73.264 kb on - strand at 73.264 kb on - strand at 73.264 kb on - strand at 73.311 kb on + strand at 73.343 kb on - strand at 73.458 kb on + strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.543 kb on + strand, within GFF6497 at 73.544 kb on - strand, within GFF6497 at 73.544 kb on - strand, within GFF6497 at 73.643 kb on - strand, within GFF6497 at 73.643 kb on - strand, within GFF6497 at 73.807 kb on + strand, within GFF6497 at 73.808 kb on - strand, within GFF6497 at 73.808 kb on - strand, within GFF6497 at 73.808 kb on - strand, within GFF6497 at 73.808 kb on - strand, within GFF6497 at 73.808 kb on - strand, within GFF6497 at 74.038 kb on + strand, within GFF6497 at 74.038 kb on + strand, within GFF6497 at 74.038 kb on + strand, within GFF6497 at 74.038 kb on + strand, within GFF6497 at 74.039 kb on - strand, within GFF6497 at 74.039 kb on - strand, within GFF6497 at 74.039 kb on - strand, within GFF6497 at 74.039 kb on - strand, within GFF6497 at 74.230 kb on + strand, within GFF6497
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 70,547 + -1.6 70,548 - +0.2 70,548 - +2.4 70,605 + -0.3 70,605 + +0.7 70,605 + -1.2 70,605 + +1.6 70,605 + -0.5 70,605 + +0.1 70,605 + -1.1 70,605 + +0.4 70,606 - -0.6 70,606 - -0.4 70,606 - +1.6 70,606 - -0.0 70,606 - -1.0 70,606 - -0.8 70,606 - -0.2 70,606 - -0.1 70,606 - -0.2 70,606 - -0.3 70,606 - -0.0 70,719 + GFF6494 0.22 -0.4 70,719 + GFF6494 0.22 -0.9 70,719 + GFF6494 0.22 -0.2 70,720 - GFF6494 0.22 +1.1 70,729 + GFF6494 0.24 +1.6 70,762 + GFF6494 0.33 -1.1 70,762 - GFF6494 0.33 +0.6 70,763 - GFF6494 0.33 +0.6 70,813 + GFF6494 0.46 -0.3 70,814 - GFF6494 0.46 -1.4 70,829 + GFF6494 0.50 -0.6 70,829 + GFF6494 0.50 -0.8 70,829 + GFF6494 0.50 -0.8 70,829 + GFF6494 0.50 -1.3 70,829 + GFF6494 0.50 -0.0 70,829 + GFF6494 0.50 -0.6 70,829 - GFF6494 0.50 -0.4 70,830 - GFF6494 0.50 -1.3 70,830 - GFF6494 0.50 +0.6 70,830 - GFF6494 0.50 +0.4 71,143 + -0.2 71,143 + -0.1 71,143 + -0.0 71,143 + -0.9 71,143 + +0.6 71,143 + +0.4 71,144 - -1.8 71,144 - +2.9 71,144 - +0.8 71,209 + GFF6495 0.23 -0.0 71,209 + GFF6495 0.23 +0.3 71,239 + GFF6495 0.30 +0.6 71,239 + GFF6495 0.30 -1.2 71,239 + GFF6495 0.30 +1.1 71,239 + GFF6495 0.30 -0.3 71,239 + GFF6495 0.30 +0.4 71,239 + GFF6495 0.30 -0.5 71,239 + GFF6495 0.30 -0.0 71,240 - GFF6495 0.31 +1.2 71,240 - GFF6495 0.31 -0.2 71,240 - GFF6495 0.31 -0.1 71,240 - GFF6495 0.31 -0.2 71,240 - GFF6495 0.31 +0.2 71,240 - GFF6495 0.31 -2.7 71,240 - GFF6495 0.31 -0.6 71,311 + GFF6495 0.48 -0.2 71,311 + GFF6495 0.48 +0.3 71,311 + GFF6495 0.48 -0.2 71,311 + GFF6495 0.48 +0.9 71,311 + GFF6495 0.48 +1.6 71,312 - GFF6495 0.49 -0.1 71,312 - GFF6495 0.49 +0.6 71,326 - GFF6495 0.52 +0.5 71,392 + GFF6495 0.69 -0.5 71,392 + GFF6495 0.69 +0.1 71,393 - GFF6495 0.69 -0.5 71,458 + GFF6495 0.86 -0.6 71,458 + GFF6495 0.86 -0.8 71,458 + GFF6495 0.86 -0.4 71,458 + GFF6495 0.86 -0.5 71,458 + GFF6495 0.86 -0.0 71,459 - GFF6495 0.86 -1.3 71,459 - GFF6495 0.86 -1.4 71,459 - GFF6495 0.86 +0.2 71,459 - GFF6495 0.86 -0.1 71,459 - GFF6495 0.86 -0.5 71,459 - GFF6495 0.86 -0.4 71,542 + -0.7 71,543 - -0.7 71,575 + -0.2 71,575 + -0.6 71,575 + +0.0 71,575 + -0.1 71,575 + -1.9 71,575 + -0.5 71,576 - +0.5 71,585 - +0.1 71,585 - -0.4 71,763 - GFF6496 0.14 +0.7 71,791 + GFF6496 0.16 -0.8 71,791 + GFF6496 0.16 +0.2 71,791 + GFF6496 0.16 +0.1 71,791 + GFF6496 0.16 +0.2 71,792 - GFF6496 0.16 -0.0 71,792 - GFF6496 0.16 -1.3 71,848 + GFF6496 0.19 +0.6 71,848 + GFF6496 0.19 +1.2 71,849 - GFF6496 0.19 -0.6 71,851 + GFF6496 0.19 -1.3 71,851 + GFF6496 0.19 -0.5 71,851 + GFF6496 0.19 -0.6 71,851 + GFF6496 0.19 -0.7 71,851 + GFF6496 0.19 +0.7 71,851 + GFF6496 0.19 +1.0 71,851 + GFF6496 0.19 +0.9 71,851 + GFF6496 0.19 +2.0 72,046 + GFF6496 0.31 +0.4 72,047 - GFF6496 0.31 -0.9 72,055 + GFF6496 0.31 -0.1 72,055 + GFF6496 0.31 +0.1 72,055 + GFF6496 0.31 -1.0 72,055 + GFF6496 0.31 +0.6 72,055 + GFF6496 0.31 -0.4 72,056 - GFF6496 0.31 -0.0 72,056 - GFF6496 0.31 -1.4 72,056 - GFF6496 0.31 -0.4 72,056 - GFF6496 0.31 -0.5 72,056 - GFF6496 0.31 -0.4 72,056 - GFF6496 0.31 -0.2 72,067 + GFF6496 0.32 -1.0 72,067 + GFF6496 0.32 +0.2 72,068 - GFF6496 0.32 -1.1 72,068 - GFF6496 0.32 +0.3 72,172 + GFF6496 0.38 -2.4 72,172 + GFF6496 0.38 -1.0 72,236 - GFF6496 0.42 +0.7 72,346 + GFF6496 0.48 -0.2 72,346 + GFF6496 0.48 -1.4 72,347 - GFF6496 0.48 +0.7 72,347 - GFF6496 0.48 +0.6 72,347 - GFF6496 0.48 +0.4 72,347 - GFF6496 0.48 -1.0 72,347 - GFF6496 0.48 -0.2 72,347 - GFF6496 0.48 +0.3 72,466 + GFF6496 0.55 -2.1 72,466 + GFF6496 0.55 +0.7 72,467 - GFF6496 0.55 +1.6 72,467 - GFF6496 0.55 -0.2 72,467 - GFF6496 0.55 -1.9 72,592 + GFF6496 0.63 +1.2 72,592 + GFF6496 0.63 +0.1 72,592 + GFF6496 0.63 +0.1 72,592 + GFF6496 0.63 +0.6 72,592 + GFF6496 0.63 -0.1 72,592 + GFF6496 0.63 -0.8 72,592 + GFF6496 0.63 -0.1 72,593 - GFF6496 0.63 -1.6 72,593 - GFF6496 0.63 +0.5 72,593 - GFF6496 0.63 +0.1 72,593 - GFF6496 0.63 +0.6 72,593 - GFF6496 0.63 -0.0 72,598 + GFF6496 0.63 +1.2 72,598 + GFF6496 0.63 +0.2 72,598 + GFF6496 0.63 -1.3 72,598 + GFF6496 0.63 -0.4 72,598 + GFF6496 0.63 -0.4 72,599 - GFF6496 0.63 -0.6 72,599 - GFF6496 0.63 -0.2 72,599 - GFF6496 0.63 -0.8 72,599 - GFF6496 0.63 +0.1 72,599 - GFF6496 0.63 +0.9 72,599 - GFF6496 0.63 -0.1 72,599 - GFF6496 0.63 +0.7 72,622 + GFF6496 0.64 +1.6 72,622 + GFF6496 0.64 -0.4 72,622 + GFF6496 0.64 +0.7 72,622 + GFF6496 0.64 +1.1 72,622 + GFF6496 0.64 +0.6 72,623 - GFF6496 0.64 +0.1 72,623 - GFF6496 0.64 -0.9 72,625 + GFF6496 0.64 +1.2 72,625 + GFF6496 0.64 -0.1 72,625 + GFF6496 0.64 -0.0 72,625 + GFF6496 0.64 -0.6 72,626 - GFF6496 0.65 -0.5 72,626 - GFF6496 0.65 +0.6 72,626 - GFF6496 0.65 -0.4 72,626 - GFF6496 0.65 +0.4 72,626 - GFF6496 0.65 +0.6 72,626 - GFF6496 0.65 -0.5 72,667 + GFF6496 0.67 -0.7 72,667 + GFF6496 0.67 +0.3 72,668 - GFF6496 0.67 -0.3 72,668 - GFF6496 0.67 -0.0 72,668 - GFF6496 0.67 -1.3 72,668 - GFF6496 0.67 +0.7 72,668 - GFF6496 0.67 +2.6 72,737 - GFF6496 0.71 +0.1 72,737 - GFF6496 0.71 -0.4 72,737 - GFF6496 0.71 -0.4 72,737 - GFF6496 0.71 +0.7 72,921 + GFF6496 0.82 -0.0 72,921 + GFF6496 0.82 -0.3 72,922 - GFF6496 0.82 -0.4 72,922 - GFF6496 0.82 -1.4 72,973 + GFF6496 0.85 -0.1 72,973 + GFF6496 0.85 +0.6 72,973 + GFF6496 0.85 -0.4 72,974 - GFF6496 0.85 -0.4 72,995 + GFF6496 0.86 -1.4 72,996 - GFF6496 0.86 +0.2 72,999 + GFF6496 0.86 +0.1 72,999 + GFF6496 0.86 +0.7 73,008 + GFF6496 0.87 -0.6 73,008 + GFF6496 0.87 -0.6 73,008 + GFF6496 0.87 -1.0 73,008 + GFF6496 0.87 -0.6 73,008 + GFF6496 0.87 -0.9 73,009 - GFF6496 0.87 -0.4 73,009 - GFF6496 0.87 +0.0 73,009 - GFF6496 0.87 +1.6 73,263 + -0.2 73,264 - -0.7 73,264 - -0.4 73,264 - -1.9 73,311 + +0.6 73,343 - +0.2 73,458 + -0.5 73,489 + -0.2 73,489 + +0.0 73,489 + -0.3 73,489 + -0.0 73,489 + -0.7 73,489 + -1.0 73,490 - +0.0 73,490 - +1.0 73,490 - +1.3 73,490 - +2.0 73,490 - -0.9 73,490 - -0.7 73,490 - -0.4 73,543 + GFF6497 0.10 +0.4 73,544 - GFF6497 0.10 -0.7 73,544 - GFF6497 0.10 +0.5 73,643 - GFF6497 0.18 -1.1 73,643 - GFF6497 0.18 -1.4 73,807 + GFF6497 0.32 +0.2 73,808 - GFF6497 0.32 +0.2 73,808 - GFF6497 0.32 +0.2 73,808 - GFF6497 0.32 -0.8 73,808 - GFF6497 0.32 +0.6 73,808 - GFF6497 0.32 -0.9 74,038 + GFF6497 0.51 +0.7 74,038 + GFF6497 0.51 -0.5 74,038 + GFF6497 0.51 -0.1 74,038 + GFF6497 0.51 +2.6 74,039 - GFF6497 0.51 -1.0 74,039 - GFF6497 0.51 +1.3 74,039 - GFF6497 0.51 -0.6 74,039 - GFF6497 0.51 +0.3 74,230 + GFF6497 0.67 -0.2
Or see this region's nucleotide sequence