Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6377 and GFF6378 are separated by 52 nucleotides GFF6378 and GFF6379 are separated by 161 nucleotides GFF6379 and GFF6380 overlap by 4 nucleotides
GFF6377 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 49,107 to 50,267
GFF6377
GFF6378 - Nicotinamidase (EC 3.5.1.19), at 50,320 to 51,045
GFF6378
GFF6379 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 51,207 to 51,941
GFF6379
GFF6380 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 51,938 to 52,729
GFF6380
Position (kb) 50
51
52 Strain fitness (log2 ratio) -2
-1
0
1 at 49.325 kb on - strand, within GFF6377 at 49.366 kb on + strand, within GFF6377 at 49.366 kb on + strand, within GFF6377 at 49.496 kb on - strand, within GFF6377 at 49.496 kb on - strand, within GFF6377 at 49.545 kb on + strand, within GFF6377 at 49.546 kb on - strand, within GFF6377 at 49.669 kb on + strand, within GFF6377 at 49.670 kb on - strand, within GFF6377 at 49.729 kb on + strand, within GFF6377 at 50.006 kb on - strand, within GFF6377 at 50.077 kb on + strand, within GFF6377 at 50.078 kb on - strand, within GFF6377 at 50.290 kb on + strand at 50.290 kb on + strand at 50.291 kb on - strand at 50.595 kb on - strand, within GFF6378 at 50.724 kb on - strand, within GFF6378 at 51.060 kb on + strand at 51.208 kb on + strand at 51.298 kb on + strand, within GFF6379 at 51.298 kb on + strand, within GFF6379 at 51.298 kb on + strand, within GFF6379 at 51.298 kb on + strand, within GFF6379 at 51.299 kb on - strand, within GFF6379 at 51.299 kb on - strand, within GFF6379 at 51.451 kb on + strand, within GFF6379 at 52.006 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 49,325 - GFF6377 0.19 -0.1 49,366 + GFF6377 0.22 -0.3 49,366 + GFF6377 0.22 -0.3 49,496 - GFF6377 0.34 -1.7 49,496 - GFF6377 0.34 -0.3 49,545 + GFF6377 0.38 -0.5 49,546 - GFF6377 0.38 +0.6 49,669 + GFF6377 0.48 -1.1 49,670 - GFF6377 0.48 -0.3 49,729 + GFF6377 0.54 -0.3 50,006 - GFF6377 0.77 -0.6 50,077 + GFF6377 0.84 +0.3 50,078 - GFF6377 0.84 -0.7 50,290 + -0.4 50,290 + -0.3 50,291 - -2.0 50,595 - GFF6378 0.38 -1.4 50,724 - GFF6378 0.56 +0.3 51,060 + -0.4 51,208 + -0.3 51,298 + GFF6379 0.12 -2.0 51,298 + GFF6379 0.12 +0.7 51,298 + GFF6379 0.12 -1.4 51,298 + GFF6379 0.12 +0.9 51,299 - GFF6379 0.13 +1.0 51,299 - GFF6379 0.13 -1.8 51,451 + GFF6379 0.33 -0.4 52,006 - -0.3
Or see this region's nucleotide sequence