Strain Fitness in Variovorax sp. SCN45 around GFF6370

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6368 and GFF6369 overlap by 4 nucleotidesGFF6369 and GFF6370 are separated by 96 nucleotidesGFF6370 and GFF6371 are separated by 68 nucleotides GFF6368 - T6SS lysozyme-like component TssE, at 38,150 to 38,698 GFF6368 GFF6369 - T6SS associated component TagJ (ImpE), at 38,695 to 39,558 GFF6369 GFF6370 - T6SS component Hcp, at 39,655 to 40,119 GFF6370 GFF6371 - T6SS component TssC (ImpC/VipB), at 40,188 to 41,696 GFF6371 Position (kb) 39 40 41Strain fitness (log2 ratio) -1 0 1at 38.795 kb on + strand, within GFF6369at 39.164 kb on - strand, within GFF6369at 39.767 kb on + strand, within GFF6370at 39.767 kb on + strand, within GFF6370at 39.767 kb on + strand, within GFF6370at 40.155 kb on - strandat 40.261 kb on + strandat 40.261 kb on + strandat 40.262 kb on - strandat 40.396 kb on + strand, within GFF6371at 40.396 kb on + strand, within GFF6371at 40.396 kb on + strand, within GFF6371at 40.483 kb on + strand, within GFF6371at 40.513 kb on + strand, within GFF6371at 40.513 kb on + strand, within GFF6371at 40.561 kb on + strand, within GFF6371at 40.561 kb on + strand, within GFF6371at 40.562 kb on - strand, within GFF6371at 40.976 kb on - strand, within GFF6371

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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38,795 + GFF6369 0.12 +0.7
39,164 - GFF6369 0.54 -0.7
39,767 + GFF6370 0.24 +0.7
39,767 + GFF6370 0.24 +0.1
39,767 + GFF6370 0.24 +0.6
40,155 - -0.8
40,261 + -0.6
40,261 + -0.2
40,262 - -1.0
40,396 + GFF6371 0.14 -0.6
40,396 + GFF6371 0.14 +1.3
40,396 + GFF6371 0.14 +0.7
40,483 + GFF6371 0.20 +1.3
40,513 + GFF6371 0.22 -0.9
40,513 + GFF6371 0.22 +0.2
40,561 + GFF6371 0.25 -1.0
40,561 + GFF6371 0.25 -0.2
40,562 - GFF6371 0.25 +0.4
40,976 - GFF6371 0.52 -0.5

Or see this region's nucleotide sequence