Strain Fitness in Variovorax sp. SCN45 around GFF6330

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6329 and GFF6330 are separated by 148 nucleotidesGFF6330 and GFF6331 overlap by 8 nucleotides GFF6329 - Leucyl-tRNA synthetase (EC 6.1.1.4), at 121,832 to 124,462 GFF6329 GFF6330 - BUG/TctC family periplasmic protein, at 124,611 to 125,585 GFF6330 GFF6331 - D-octopine dehydrogenase (EC 1.5.1.11), at 125,578 to 126,648 GFF6331 Position (kb) 124 125 126Strain fitness (log2 ratio) -2 -1 0 1at 124.546 kb on - strandat 124.546 kb on - strandat 124.560 kb on + strandat 124.560 kb on + strandat 124.560 kb on + strandat 124.560 kb on + strandat 124.561 kb on - strandat 124.561 kb on - strandat 124.561 kb on - strandat 124.561 kb on - strandat 124.935 kb on + strand, within GFF6330at 124.935 kb on + strand, within GFF6330at 124.936 kb on - strand, within GFF6330at 124.936 kb on - strand, within GFF6330at 124.936 kb on - strand, within GFF6330at 125.016 kb on + strand, within GFF6330at 125.016 kb on + strand, within GFF6330at 125.017 kb on - strand, within GFF6330at 125.073 kb on + strand, within GFF6330at 125.073 kb on + strand, within GFF6330at 125.073 kb on + strand, within GFF6330at 125.073 kb on + strand, within GFF6330at 125.073 kb on + strand, within GFF6330at 125.073 kb on + strand, within GFF6330at 125.074 kb on - strand, within GFF6330at 125.074 kb on - strand, within GFF6330at 125.074 kb on - strand, within GFF6330at 125.074 kb on - strand, within GFF6330at 125.074 kb on - strand, within GFF6330at 125.074 kb on - strand, within GFF6330at 125.074 kb on - strand, within GFF6330at 125.583 kb on + strandat 125.584 kb on - strandat 125.584 kb on - strandat 125.584 kb on - strandat 125.593 kb on + strandat 125.593 kb on + strandat 125.594 kb on - strandat 125.594 kb on - strandat 125.807 kb on + strand, within GFF6331at 125.807 kb on + strand, within GFF6331at 125.807 kb on + strand, within GFF6331at 125.807 kb on + strand, within GFF6331at 125.807 kb on + strand, within GFF6331at 125.807 kb on + strand, within GFF6331at 125.807 kb on + strand, within GFF6331at 125.807 kb on + strand, within GFF6331at 125.808 kb on - strand, within GFF6331at 125.808 kb on - strand, within GFF6331at 125.808 kb on - strand, within GFF6331at 125.808 kb on - strand, within GFF6331at 125.808 kb on - strand, within GFF6331at 125.808 kb on - strand, within GFF6331at 125.808 kb on - strand, within GFF6331at 125.920 kb on + strand, within GFF6331at 125.920 kb on + strand, within GFF6331at 125.920 kb on + strand, within GFF6331at 125.920 kb on + strand, within GFF6331at 125.920 kb on + strand, within GFF6331at 125.921 kb on - strand, within GFF6331at 125.921 kb on - strand, within GFF6331at 125.921 kb on - strand, within GFF6331at 125.921 kb on - strand, within GFF6331at 125.921 kb on - strand, within GFF6331at 126.069 kb on + strand, within GFF6331at 126.070 kb on - strand, within GFF6331at 126.235 kb on + strand, within GFF6331at 126.236 kb on - strand, within GFF6331at 126.318 kb on - strand, within GFF6331at 126.318 kb on - strand, within GFF6331at 126.448 kb on + strand, within GFF6331at 126.448 kb on + strand, within GFF6331at 126.448 kb on + strand, within GFF6331at 126.449 kb on - strand, within GFF6331at 126.449 kb on - strand, within GFF6331at 126.449 kb on - strand, within GFF6331at 126.449 kb on - strand, within GFF6331at 126.469 kb on + strand, within GFF6331at 126.469 kb on + strand, within GFF6331

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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124,546 - -0.5
124,546 - -1.9
124,560 + +1.2
124,560 + -0.1
124,560 + -1.3
124,560 + -0.2
124,561 - +1.0
124,561 - +0.1
124,561 - +0.4
124,561 - -0.6
124,935 + GFF6330 0.33 +0.9
124,935 + GFF6330 0.33 +0.3
124,936 - GFF6330 0.33 -0.4
124,936 - GFF6330 0.33 +1.3
124,936 - GFF6330 0.33 -0.0
125,016 + GFF6330 0.42 -0.1
125,016 + GFF6330 0.42 +1.1
125,017 - GFF6330 0.42 -0.1
125,073 + GFF6330 0.47 -0.4
125,073 + GFF6330 0.47 -0.4
125,073 + GFF6330 0.47 +0.7
125,073 + GFF6330 0.47 +0.1
125,073 + GFF6330 0.47 +0.1
125,073 + GFF6330 0.47 +0.3
125,074 - GFF6330 0.47 -0.3
125,074 - GFF6330 0.47 +0.1
125,074 - GFF6330 0.47 -0.3
125,074 - GFF6330 0.47 +0.2
125,074 - GFF6330 0.47 +0.2
125,074 - GFF6330 0.47 -0.0
125,074 - GFF6330 0.47 +0.3
125,583 + -0.1
125,584 - -0.9
125,584 - -0.6
125,584 - +0.2
125,593 + -0.1
125,593 + -1.6
125,594 - +0.2
125,594 - -2.0
125,807 + GFF6331 0.21 -0.0
125,807 + GFF6331 0.21 -0.2
125,807 + GFF6331 0.21 +0.3
125,807 + GFF6331 0.21 -1.5
125,807 + GFF6331 0.21 -1.0
125,807 + GFF6331 0.21 +0.1
125,807 + GFF6331 0.21 -0.7
125,807 + GFF6331 0.21 -0.3
125,808 - GFF6331 0.21 -1.2
125,808 - GFF6331 0.21 +0.9
125,808 - GFF6331 0.21 +0.8
125,808 - GFF6331 0.21 -0.1
125,808 - GFF6331 0.21 +0.4
125,808 - GFF6331 0.21 +0.1
125,808 - GFF6331 0.21 +1.0
125,920 + GFF6331 0.32 +0.9
125,920 + GFF6331 0.32 -2.4
125,920 + GFF6331 0.32 -0.1
125,920 + GFF6331 0.32 -0.7
125,920 + GFF6331 0.32 -1.0
125,921 - GFF6331 0.32 +0.1
125,921 - GFF6331 0.32 -0.7
125,921 - GFF6331 0.32 -0.6
125,921 - GFF6331 0.32 -0.5
125,921 - GFF6331 0.32 +1.2
126,069 + GFF6331 0.46 +0.3
126,070 - GFF6331 0.46 +0.4
126,235 + GFF6331 0.61 -1.8
126,236 - GFF6331 0.61 +0.5
126,318 - GFF6331 0.69 -0.1
126,318 - GFF6331 0.69 -0.1
126,448 + GFF6331 0.81 +0.9
126,448 + GFF6331 0.81 +1.2
126,448 + GFF6331 0.81 +0.5
126,449 - GFF6331 0.81 -0.7
126,449 - GFF6331 0.81 -0.1
126,449 - GFF6331 0.81 +0.2
126,449 - GFF6331 0.81 -0.3
126,469 + GFF6331 0.83 +1.3
126,469 + GFF6331 0.83 +0.2

Or see this region's nucleotide sequence