Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6193 and GFF6194 are separated by 46 nucleotides GFF6194 and GFF6195 overlap by 4 nucleotides
GFF6193 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 99,567 to 101,060
GFF6193
GFF6194 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 101,107 to 101,922
GFF6194
GFF6195 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 101,919 to 102,926
GFF6195
Position (kb)
101
102 Strain fitness (log2 ratio)
-1
0
1
2 at 100.773 kb on + strand, within GFF6193 at 100.773 kb on + strand, within GFF6193 at 100.773 kb on + strand, within GFF6193 at 100.773 kb on + strand, within GFF6193 at 100.774 kb on - strand, within GFF6193 at 100.882 kb on + strand, within GFF6193 at 100.883 kb on - strand, within GFF6193 at 100.883 kb on - strand, within GFF6193 at 100.883 kb on - strand, within GFF6193 at 100.883 kb on - strand, within GFF6193 at 100.883 kb on - strand, within GFF6193 at 101.066 kb on - strand at 101.153 kb on + strand at 101.153 kb on + strand at 101.154 kb on - strand at 101.154 kb on - strand at 101.280 kb on - strand, within GFF6194 at 101.280 kb on - strand, within GFF6194 at 101.280 kb on - strand, within GFF6194 at 101.280 kb on - strand, within GFF6194 at 101.598 kb on - strand, within GFF6194 at 101.861 kb on + strand at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.248 kb on - strand, within GFF6195
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 100,773 + GFF6193 0.81 -1.0 100,773 + GFF6193 0.81 -0.2 100,773 + GFF6193 0.81 +0.7 100,773 + GFF6193 0.81 +1.4 100,774 - GFF6193 0.81 +0.1 100,882 + GFF6193 0.88 +0.8 100,883 - GFF6193 0.88 -0.5 100,883 - GFF6193 0.88 -0.4 100,883 - GFF6193 0.88 +0.2 100,883 - GFF6193 0.88 +1.3 100,883 - GFF6193 0.88 -0.0 101,066 - +1.1 101,153 + +1.1 101,153 + -0.0 101,154 - -0.1 101,154 - -0.3 101,280 - GFF6194 0.21 -1.3 101,280 - GFF6194 0.21 +1.7 101,280 - GFF6194 0.21 +0.8 101,280 - GFF6194 0.21 +1.8 101,598 - GFF6194 0.60 +0.2 101,861 + +0.9 102,247 + GFF6195 0.33 -0.7 102,247 + GFF6195 0.33 +0.1 102,247 + GFF6195 0.33 -0.7 102,247 + GFF6195 0.33 +0.6 102,247 + GFF6195 0.33 +0.9 102,248 - GFF6195 0.33 -0.0
Or see this region's nucleotide sequence