Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6160 and GFF6161 are separated by 19 nucleotides GFF6161 and GFF6162 overlap by 4 nucleotides GFF6162 and GFF6163 are separated by 11 nucleotides GFF6163 and GFF6164 are separated by 4 nucleotides
GFF6160 - Dehydrogenase molybdenum cofactor insertion protein, XdhC/CoxF family, at 65,911 to 66,933
GFF6160
GFF6161 - Hydroxymethylpyrimidine ABC transporter, ATPase component, at 66,953 to 67,756
GFF6161
GFF6162 - Dihydrodipicolinate synthase family protein RB7999, at 67,753 to 68,820
GFF6162
GFF6163 - hypothetical protein, at 68,832 to 69,686
GFF6163
GFF6164 - FIG01094124: hypothetical protein, at 69,691 to 70,740
GFF6164
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 66.796 kb on + strand, within GFF6160 at 66.796 kb on + strand, within GFF6160 at 66.796 kb on + strand, within GFF6160 at 66.796 kb on + strand, within GFF6160 at 66.797 kb on - strand, within GFF6160 at 66.797 kb on - strand, within GFF6160 at 66.797 kb on - strand, within GFF6160 at 66.975 kb on + strand at 66.975 kb on + strand at 66.975 kb on + strand at 66.975 kb on + strand at 66.975 kb on + strand at 66.976 kb on - strand at 66.976 kb on - strand at 66.976 kb on - strand at 66.976 kb on - strand at 66.990 kb on + strand at 66.990 kb on + strand at 66.991 kb on - strand at 67.311 kb on + strand, within GFF6161 at 67.312 kb on - strand, within GFF6161 at 67.312 kb on - strand, within GFF6161 at 67.677 kb on + strand at 67.678 kb on - strand at 67.820 kb on + strand at 67.820 kb on + strand at 67.821 kb on - strand at 67.822 kb on + strand at 67.822 kb on + strand at 67.823 kb on - strand at 67.823 kb on - strand at 68.122 kb on + strand, within GFF6162 at 68.122 kb on + strand, within GFF6162 at 68.122 kb on + strand, within GFF6162 at 68.122 kb on + strand, within GFF6162 at 68.123 kb on - strand, within GFF6162 at 68.370 kb on - strand, within GFF6162 at 68.475 kb on - strand, within GFF6162 at 68.606 kb on + strand, within GFF6162 at 68.607 kb on - strand, within GFF6162 at 68.684 kb on + strand, within GFF6162 at 68.685 kb on - strand, within GFF6162 at 69.343 kb on + strand, within GFF6163 at 69.343 kb on + strand, within GFF6163 at 69.343 kb on + strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.695 kb on + strand at 69.695 kb on + strand at 69.695 kb on + strand at 69.696 kb on - strand at 69.696 kb on - strand at 69.734 kb on + strand at 69.735 kb on - strand at 69.735 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 66,796 + GFF6160 0.87 -1.0 66,796 + GFF6160 0.87 +0.3 66,796 + GFF6160 0.87 +0.1 66,796 + GFF6160 0.87 +0.2 66,797 - GFF6160 0.87 -0.3 66,797 - GFF6160 0.87 -0.6 66,797 - GFF6160 0.87 -0.8 66,975 + +0.2 66,975 + +0.3 66,975 + -1.5 66,975 + -0.2 66,975 + +1.0 66,976 - -1.4 66,976 - +2.3 66,976 - -0.2 66,976 - -0.3 66,990 + +0.5 66,990 + -0.2 66,991 - +0.2 67,311 + GFF6161 0.45 -1.0 67,312 - GFF6161 0.45 -0.8 67,312 - GFF6161 0.45 -0.5 67,677 + -0.3 67,678 - +0.8 67,820 + -0.3 67,820 + -0.2 67,821 - -1.9 67,822 + -1.5 67,822 + -0.9 67,823 - -0.2 67,823 - +0.9 68,122 + GFF6162 0.35 +0.1 68,122 + GFF6162 0.35 -2.4 68,122 + GFF6162 0.35 +0.3 68,122 + GFF6162 0.35 +1.2 68,123 - GFF6162 0.35 -0.2 68,370 - GFF6162 0.58 +0.1 68,475 - GFF6162 0.68 -0.9 68,606 + GFF6162 0.80 +0.3 68,607 - GFF6162 0.80 -2.9 68,684 + GFF6162 0.87 -1.5 68,685 - GFF6162 0.87 +0.2 69,343 + GFF6163 0.60 +0.0 69,343 + GFF6163 0.60 -1.5 69,343 + GFF6163 0.60 -0.2 69,344 - GFF6163 0.60 -0.1 69,344 - GFF6163 0.60 -1.6 69,344 - GFF6163 0.60 +0.0 69,695 + +2.3 69,695 + -0.8 69,695 + -0.6 69,696 - -0.3 69,696 - -0.6 69,734 + -0.3 69,735 - +0.9 69,735 - -2.6
Or see this region's nucleotide sequence