Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6113 and GFF6114 are separated by 19 nucleotides GFF6114 and GFF6115 are separated by 30 nucleotides GFF6115 and GFF6116 are separated by 50 nucleotides
GFF6113 - Dipeptide ABC transporter, permease protein DppC (TC 3.A.1.5.2), at 12,204 to 13,106
GFF6113
GFF6114 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 13,126 to 14,061
GFF6114
GFF6115 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 14,092 to 15,681
GFF6115
GFF6116 - MlrC, at 15,732 to 17,186
GFF6116
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1 at 13.131 kb on - strand at 13.131 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.430 kb on + strand, within GFF6114 at 13.430 kb on + strand, within GFF6114 at 13.431 kb on - strand, within GFF6114 at 13.431 kb on - strand, within GFF6114 at 13.490 kb on + strand, within GFF6114 at 13.491 kb on - strand, within GFF6114 at 13.491 kb on - strand, within GFF6114 at 13.491 kb on - strand, within GFF6114 at 13.566 kb on - strand, within GFF6114 at 13.566 kb on - strand, within GFF6114 at 13.607 kb on + strand, within GFF6114 at 14.054 kb on + strand at 14.055 kb on - strand at 14.093 kb on + strand at 14.094 kb on - strand at 14.168 kb on + strand at 14.198 kb on + strand at 14.199 kb on - strand at 14.199 kb on - strand at 14.199 kb on - strand at 14.213 kb on + strand at 14.351 kb on + strand, within GFF6115 at 14.352 kb on - strand, within GFF6115 at 14.402 kb on + strand, within GFF6115 at 14.402 kb on + strand, within GFF6115 at 14.402 kb on + strand, within GFF6115 at 14.402 kb on + strand, within GFF6115 at 14.403 kb on - strand, within GFF6115 at 14.403 kb on - strand, within GFF6115 at 14.403 kb on - strand, within GFF6115 at 14.543 kb on + strand, within GFF6115 at 14.544 kb on - strand, within GFF6115 at 14.589 kb on - strand, within GFF6115 at 14.687 kb on + strand, within GFF6115 at 14.687 kb on + strand, within GFF6115 at 14.688 kb on - strand, within GFF6115 at 14.688 kb on - strand, within GFF6115 at 14.688 kb on - strand, within GFF6115 at 14.688 kb on - strand, within GFF6115 at 14.688 kb on - strand, within GFF6115 at 14.699 kb on + strand, within GFF6115 at 14.699 kb on + strand, within GFF6115 at 14.889 kb on - strand, within GFF6115 at 14.966 kb on + strand, within GFF6115 at 14.967 kb on - strand, within GFF6115 at 15.000 kb on - strand, within GFF6115 at 15.041 kb on + strand, within GFF6115 at 15.041 kb on + strand, within GFF6115 at 15.041 kb on + strand, within GFF6115 at 15.041 kb on + strand, within GFF6115 at 15.041 kb on + strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.042 kb on - strand, within GFF6115 at 15.435 kb on - strand, within GFF6115 at 15.435 kb on - strand, within GFF6115 at 15.504 kb on - strand, within GFF6115 at 15.524 kb on + strand at 15.524 kb on + strand at 15.524 kb on + strand at 15.525 kb on - strand at 15.781 kb on + strand at 15.829 kb on + strand at 15.865 kb on + strand at 15.865 kb on + strand at 15.865 kb on + strand at 15.865 kb on + strand at 15.865 kb on + strand at 15.865 kb on + strand at 15.865 kb on + strand at 15.866 kb on - strand at 15.866 kb on - strand at 15.866 kb on - strand at 16.357 kb on + strand, within GFF6116 at 16.357 kb on + strand, within GFF6116 at 16.447 kb on + strand, within GFF6116 at 16.447 kb on + strand, within GFF6116
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 13,131 - +0.0 13,131 - -0.1 13,191 - +0.1 13,191 - +0.9 13,191 - -2.4 13,430 + GFF6114 0.32 -1.0 13,430 + GFF6114 0.32 -0.0 13,431 - GFF6114 0.33 +0.5 13,431 - GFF6114 0.33 -0.2 13,490 + GFF6114 0.39 -1.0 13,491 - GFF6114 0.39 -0.1 13,491 - GFF6114 0.39 +0.2 13,491 - GFF6114 0.39 +0.2 13,566 - GFF6114 0.47 -0.2 13,566 - GFF6114 0.47 -0.4 13,607 + GFF6114 0.51 +0.1 14,054 + -0.8 14,055 - -1.0 14,093 + +0.3 14,094 - -0.0 14,168 + -0.3 14,198 + +0.6 14,199 - -0.4 14,199 - -0.8 14,199 - -0.0 14,213 + +0.2 14,351 + GFF6115 0.16 -0.2 14,352 - GFF6115 0.16 -0.1 14,402 + GFF6115 0.19 -0.2 14,402 + GFF6115 0.19 +1.0 14,402 + GFF6115 0.19 -0.7 14,402 + GFF6115 0.19 -0.6 14,403 - GFF6115 0.20 +0.5 14,403 - GFF6115 0.20 -0.9 14,403 - GFF6115 0.20 +1.1 14,543 + GFF6115 0.28 -1.0 14,544 - GFF6115 0.28 -0.3 14,589 - GFF6115 0.31 -0.3 14,687 + GFF6115 0.37 -0.7 14,687 + GFF6115 0.37 -1.6 14,688 - GFF6115 0.37 -0.7 14,688 - GFF6115 0.37 -0.1 14,688 - GFF6115 0.37 -0.0 14,688 - GFF6115 0.37 -0.7 14,688 - GFF6115 0.37 +0.1 14,699 + GFF6115 0.38 +0.0 14,699 + GFF6115 0.38 +1.2 14,889 - GFF6115 0.50 -0.5 14,966 + GFF6115 0.55 -0.5 14,967 - GFF6115 0.55 -0.5 15,000 - GFF6115 0.57 +0.1 15,041 + GFF6115 0.60 -0.9 15,041 + GFF6115 0.60 -0.3 15,041 + GFF6115 0.60 -0.0 15,041 + GFF6115 0.60 -0.4 15,041 + GFF6115 0.60 -2.0 15,042 - GFF6115 0.60 -0.1 15,042 - GFF6115 0.60 -1.2 15,042 - GFF6115 0.60 +0.3 15,042 - GFF6115 0.60 +1.5 15,042 - GFF6115 0.60 -0.4 15,042 - GFF6115 0.60 +0.8 15,042 - GFF6115 0.60 -0.5 15,042 - GFF6115 0.60 -0.5 15,435 - GFF6115 0.84 -0.6 15,435 - GFF6115 0.84 -1.5 15,504 - GFF6115 0.89 -2.4 15,524 + -0.0 15,524 + +0.0 15,524 + +0.2 15,525 - -0.1 15,781 + +0.4 15,829 + -0.2 15,865 + -1.0 15,865 + +1.5 15,865 + +0.3 15,865 + +0.1 15,865 + +0.1 15,865 + -0.9 15,865 + -0.5 15,866 - +0.2 15,866 - -0.6 15,866 - -0.9 16,357 + GFF6116 0.43 -0.4 16,357 + GFF6116 0.43 -0.6 16,447 + GFF6116 0.49 -0.4 16,447 + GFF6116 0.49 -1.5
Or see this region's nucleotide sequence