Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF597 and GFF598 are separated by 116 nucleotides GFF598 and GFF599 are separated by 46 nucleotides GFF599 and GFF600 are separated by 60 nucleotides GFF600 and GFF601 are separated by 22 nucleotides
GFF597 - Uncharacterized MFS-type transporter, at 643,164 to 644,714
GFF597
GFF598 - no description, at 644,831 to 645,157
GFF598
GFF599 - no description, at 645,204 to 645,434
GFF599
GFF600 - Metal-dependent phosphohydrolase, HD subdomain, at 645,495 to 646,085
GFF600
GFF601 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 646,108 to 646,875
GFF601
Position (kb) 644
645
646 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 643.853 kb on - strand, within GFF597 at 643.853 kb on - strand, within GFF597 at 643.951 kb on - strand, within GFF597 at 643.951 kb on - strand, within GFF597 at 644.355 kb on + strand, within GFF597 at 644.355 kb on + strand, within GFF597 at 644.355 kb on + strand, within GFF597 at 644.355 kb on + strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.356 kb on - strand, within GFF597 at 644.412 kb on + strand, within GFF597 at 644.412 kb on + strand, within GFF597 at 644.412 kb on + strand, within GFF597 at 644.412 kb on + strand, within GFF597 at 644.412 kb on + strand, within GFF597 at 644.712 kb on + strand at 644.713 kb on - strand at 644.750 kb on + strand at 644.830 kb on + strand at 644.830 kb on + strand at 644.928 kb on + strand, within GFF598 at 644.928 kb on + strand, within GFF598 at 644.928 kb on + strand, within GFF598 at 645.186 kb on + strand at 645.186 kb on + strand at 645.186 kb on + strand at 645.187 kb on - strand at 645.406 kb on - strand, within GFF599 at 645.458 kb on + strand at 645.458 kb on + strand at 645.458 kb on + strand at 645.458 kb on + strand at 645.458 kb on + strand at 645.458 kb on + strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.459 kb on - strand at 645.633 kb on + strand, within GFF600 at 645.634 kb on - strand, within GFF600 at 645.634 kb on - strand, within GFF600 at 645.634 kb on - strand, within GFF600 at 645.657 kb on + strand, within GFF600 at 645.658 kb on - strand, within GFF600 at 645.658 kb on - strand, within GFF600 at 645.658 kb on - strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.714 kb on + strand, within GFF600 at 645.715 kb on - strand, within GFF600 at 645.715 kb on - strand, within GFF600 at 645.715 kb on - strand, within GFF600 at 645.715 kb on - strand, within GFF600 at 645.715 kb on - strand, within GFF600 at 645.715 kb on - strand, within GFF600 at 645.715 kb on - strand, within GFF600 at 645.858 kb on + strand, within GFF600 at 645.858 kb on + strand, within GFF600 at 645.858 kb on + strand, within GFF600 at 645.858 kb on + strand, within GFF600 at 645.859 kb on - strand, within GFF600 at 645.859 kb on - strand, within GFF600 at 645.859 kb on - strand, within GFF600 at 645.922 kb on - strand, within GFF600 at 645.951 kb on + strand, within GFF600 at 646.050 kb on + strand at 646.050 kb on + strand at 646.050 kb on + strand at 646.050 kb on + strand at 646.050 kb on + strand at 646.050 kb on + strand at 646.050 kb on + strand at 646.050 kb on + strand at 646.051 kb on - strand at 646.051 kb on - strand at 646.051 kb on - strand at 646.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 643,853 - GFF597 0.44 -1.0 643,853 - GFF597 0.44 -1.9 643,951 - GFF597 0.51 -0.6 643,951 - GFF597 0.51 +0.1 644,355 + GFF597 0.77 +1.4 644,355 + GFF597 0.77 +0.2 644,355 + GFF597 0.77 -0.3 644,355 + GFF597 0.77 -0.2 644,356 - GFF597 0.77 +0.3 644,356 - GFF597 0.77 +0.8 644,356 - GFF597 0.77 -1.0 644,356 - GFF597 0.77 -0.6 644,356 - GFF597 0.77 -0.2 644,356 - GFF597 0.77 -0.4 644,356 - GFF597 0.77 -0.1 644,356 - GFF597 0.77 -0.5 644,412 + GFF597 0.80 -0.4 644,412 + GFF597 0.80 -0.5 644,412 + GFF597 0.80 +0.5 644,412 + GFF597 0.80 -0.0 644,412 + GFF597 0.80 -1.1 644,712 + -1.0 644,713 - -1.7 644,750 + +0.0 644,830 + -0.7 644,830 + +1.2 644,928 + GFF598 0.30 -1.3 644,928 + GFF598 0.30 -1.5 644,928 + GFF598 0.30 -0.9 645,186 + -0.1 645,186 + -1.0 645,186 + -0.3 645,187 - -0.3 645,406 - GFF599 0.87 -1.3 645,458 + -0.3 645,458 + +0.2 645,458 + -0.0 645,458 + -0.9 645,458 + +1.3 645,458 + -0.2 645,459 - -0.9 645,459 - -2.9 645,459 - +0.3 645,459 - +0.2 645,459 - -0.0 645,459 - +0.3 645,459 - +0.3 645,459 - -1.0 645,459 - -1.1 645,459 - +1.3 645,459 - -1.4 645,459 - -1.2 645,633 + GFF600 0.23 -1.0 645,634 - GFF600 0.24 -2.2 645,634 - GFF600 0.24 +0.1 645,634 - GFF600 0.24 -1.0 645,657 + GFF600 0.27 -0.9 645,658 - GFF600 0.28 +0.6 645,658 - GFF600 0.28 +0.1 645,658 - GFF600 0.28 -0.0 645,714 + GFF600 0.37 -0.2 645,714 + GFF600 0.37 -0.1 645,714 + GFF600 0.37 -1.5 645,714 + GFF600 0.37 +1.6 645,714 + GFF600 0.37 +1.3 645,714 + GFF600 0.37 -0.9 645,714 + GFF600 0.37 -0.1 645,714 + GFF600 0.37 -0.6 645,714 + GFF600 0.37 +0.5 645,714 + GFF600 0.37 +0.1 645,714 + GFF600 0.37 -0.5 645,715 - GFF600 0.37 -0.5 645,715 - GFF600 0.37 +0.0 645,715 - GFF600 0.37 -0.3 645,715 - GFF600 0.37 -2.7 645,715 - GFF600 0.37 -0.8 645,715 - GFF600 0.37 -1.0 645,715 - GFF600 0.37 -0.4 645,858 + GFF600 0.61 -0.3 645,858 + GFF600 0.61 +0.2 645,858 + GFF600 0.61 +0.5 645,858 + GFF600 0.61 +0.4 645,859 - GFF600 0.62 +1.2 645,859 - GFF600 0.62 +0.3 645,859 - GFF600 0.62 -0.3 645,922 - GFF600 0.72 -0.1 645,951 + GFF600 0.77 -1.1 646,050 + -0.5 646,050 + +0.9 646,050 + +0.4 646,050 + -0.9 646,050 + +0.3 646,050 + -1.5 646,050 + -0.2 646,050 + -0.1 646,051 - -0.6 646,051 - -1.2 646,051 - -1.9 646,051 - -0.0
Or see this region's nucleotide sequence