Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF575 and GFF576 are separated by 15 nucleotides GFF576 and GFF577 are separated by 3 nucleotides GFF577 and GFF578 are separated by 89 nucleotides
GFF575 - Tol-Pal system protein TolQ, at 620,197 to 620,919
GFF575
GFF576 - Tol biopolymer transport system, TolR protein, at 620,935 to 621,372
GFF576
GFF577 - TolA protein, at 621,376 to 622,299
GFF577
GFF578 - Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129), at 622,389 to 624,119
GFF578
Position (kb) 621
622
623 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 620.468 kb on - strand, within GFF575 at 620.468 kb on - strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.773 kb on + strand, within GFF575 at 620.773 kb on + strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.602 kb on + strand, within GFF577 at 622.036 kb on + strand, within GFF577 at 622.036 kb on + strand, within GFF577 at 622.037 kb on - strand, within GFF577 at 622.328 kb on + strand at 622.328 kb on + strand at 622.329 kb on - strand at 622.329 kb on - strand at 622.329 kb on - strand at 622.377 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.518 kb on + strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.519 kb on - strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.522 kb on + strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.523 kb on - strand at 622.826 kb on + strand, within GFF578 at 622.827 kb on - strand, within GFF578 at 622.827 kb on - strand, within GFF578 at 622.827 kb on - strand, within GFF578 at 622.827 kb on - strand, within GFF578 at 622.827 kb on - strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.860 kb on + strand, within GFF578 at 622.861 kb on - strand, within GFF578 at 622.861 kb on - strand, within GFF578 at 622.861 kb on - strand, within GFF578 at 622.861 kb on - strand, within GFF578 at 622.861 kb on - strand, within GFF578 at 622.861 kb on - strand, within GFF578 at 622.861 kb on - strand, within GFF578 at 623.118 kb on + strand, within GFF578 at 623.119 kb on - strand, within GFF578 at 623.217 kb on + strand, within GFF578 at 623.217 kb on + strand, within GFF578 at 623.217 kb on + strand, within GFF578 at 623.217 kb on + strand, within GFF578 at 623.218 kb on - strand, within GFF578 at 623.218 kb on - strand, within GFF578 at 623.218 kb on - strand, within GFF578 at 623.226 kb on + strand, within GFF578 at 623.226 kb on + strand, within GFF578 at 623.226 kb on + strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578 at 623.227 kb on - strand, within GFF578
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 620,468 - GFF575 0.37 -1.2 620,468 - GFF575 0.37 -0.9 620,614 + GFF575 0.58 +0.6 620,614 + GFF575 0.58 -1.4 620,614 + GFF575 0.58 -1.3 620,614 + GFF575 0.58 +1.3 620,614 + GFF575 0.58 -1.3 620,614 + GFF575 0.58 -2.0 620,614 + GFF575 0.58 -0.5 620,614 + GFF575 0.58 -2.1 620,614 + GFF575 0.58 +0.6 620,614 + GFF575 0.58 -1.5 620,614 + GFF575 0.58 -1.0 620,614 + GFF575 0.58 -1.7 620,615 - GFF575 0.58 -1.8 620,615 - GFF575 0.58 -1.0 620,615 - GFF575 0.58 -2.3 620,615 - GFF575 0.58 -0.6 620,615 - GFF575 0.58 -1.1 620,615 - GFF575 0.58 -1.0 620,615 - GFF575 0.58 -0.5 620,615 - GFF575 0.58 -2.8 620,615 - GFF575 0.58 -2.1 620,615 - GFF575 0.58 +0.1 620,615 - GFF575 0.58 -0.0 620,615 - GFF575 0.58 -1.2 620,615 - GFF575 0.58 -1.3 620,615 - GFF575 0.58 -3.0 620,615 - GFF575 0.58 +0.3 620,615 - GFF575 0.58 -0.1 620,615 - GFF575 0.58 -2.7 620,615 - GFF575 0.58 -1.0 620,615 - GFF575 0.58 -1.7 620,773 + GFF575 0.80 -2.2 620,773 + GFF575 0.80 -0.7 620,774 - GFF575 0.80 -0.5 620,774 - GFF575 0.80 -3.2 620,774 - GFF575 0.80 -0.7 620,824 + GFF575 0.87 -1.0 620,824 + GFF575 0.87 -1.7 620,824 + GFF575 0.87 -1.9 620,824 + GFF575 0.87 -2.8 620,824 + GFF575 0.87 -1.7 620,824 + GFF575 0.87 -3.5 620,824 + GFF575 0.87 -0.3 620,824 + GFF575 0.87 +0.3 620,825 - GFF575 0.87 -0.3 620,825 - GFF575 0.87 -1.7 620,825 - GFF575 0.87 -2.8 620,825 - GFF575 0.87 -1.9 620,825 - GFF575 0.87 -2.0 620,825 - GFF575 0.87 -1.0 620,825 - GFF575 0.87 -0.4 620,825 - GFF575 0.87 -0.5 621,283 + GFF576 0.79 -2.1 621,283 + GFF576 0.79 -1.7 621,283 + GFF576 0.79 -0.3 621,283 + GFF576 0.79 -0.1 621,283 + GFF576 0.79 -3.7 621,284 - GFF576 0.80 -0.7 621,284 - GFF576 0.80 +0.2 621,284 - GFF576 0.80 -0.8 621,370 + -1.0 621,370 + -0.9 621,370 + +0.5 621,370 + -0.3 621,370 + +0.5 621,370 + -0.5 621,370 + -0.2 621,370 + -1.2 621,370 + +0.5 621,371 - +0.0 621,371 - -0.7 621,371 - -0.5 621,371 - -0.7 621,371 - -0.6 621,371 - -0.7 621,371 - +1.3 621,371 - +0.8 621,371 - +0.3 621,371 - +0.1 621,371 - -0.4 621,371 - -0.9 621,602 + GFF577 0.24 -0.7 622,036 + GFF577 0.71 -0.8 622,036 + GFF577 0.71 -1.7 622,037 - GFF577 0.72 -2.3 622,328 + -1.1 622,328 + -0.7 622,329 - +0.6 622,329 - +0.6 622,329 - -0.4 622,377 + -0.1 622,518 + +1.1 622,518 + +0.1 622,518 + -0.5 622,518 + -0.2 622,518 + -0.2 622,518 + -0.9 622,518 + -0.3 622,518 + +1.3 622,518 + -1.5 622,519 - -0.3 622,519 - -1.6 622,519 - +1.3 622,519 - -0.5 622,519 - +0.4 622,519 - -1.1 622,519 - -0.3 622,519 - -0.5 622,519 - +0.3 622,519 - -0.1 622,519 - -0.4 622,519 - -1.3 622,519 - +1.8 622,519 - +0.1 622,522 + -0.3 622,522 + +0.1 622,522 + +0.6 622,522 + -0.1 622,522 + +1.0 622,522 + -0.4 622,522 + -0.9 622,522 + -0.5 622,522 + +1.3 622,522 + -1.1 622,523 - +1.8 622,523 - +0.0 622,523 - -0.4 622,523 - -0.1 622,523 - +0.5 622,523 - +1.3 622,523 - -0.7 622,523 - -0.4 622,523 - +0.1 622,523 - +0.9 622,523 - -0.1 622,523 - -0.5 622,523 - +0.9 622,523 - +0.7 622,523 - -0.2 622,826 + GFF578 0.25 -1.5 622,827 - GFF578 0.25 -0.1 622,827 - GFF578 0.25 -2.1 622,827 - GFF578 0.25 -0.1 622,827 - GFF578 0.25 -1.4 622,827 - GFF578 0.25 +0.3 622,860 + GFF578 0.27 -0.6 622,860 + GFF578 0.27 +0.1 622,860 + GFF578 0.27 -1.0 622,860 + GFF578 0.27 -0.9 622,860 + GFF578 0.27 +0.6 622,860 + GFF578 0.27 -2.3 622,860 + GFF578 0.27 -0.5 622,860 + GFF578 0.27 +1.9 622,860 + GFF578 0.27 +0.0 622,861 - GFF578 0.27 -1.0 622,861 - GFF578 0.27 +1.5 622,861 - GFF578 0.27 -1.0 622,861 - GFF578 0.27 +0.4 622,861 - GFF578 0.27 -0.5 622,861 - GFF578 0.27 -0.1 622,861 - GFF578 0.27 +0.3 623,118 + GFF578 0.42 +0.7 623,119 - GFF578 0.42 -2.2 623,217 + GFF578 0.48 +0.3 623,217 + GFF578 0.48 -0.6 623,217 + GFF578 0.48 -0.7 623,217 + GFF578 0.48 +0.3 623,218 - GFF578 0.48 +0.1 623,218 - GFF578 0.48 +2.3 623,218 - GFF578 0.48 -1.0 623,226 + GFF578 0.48 -1.0 623,226 + GFF578 0.48 -0.0 623,226 + GFF578 0.48 +0.3 623,227 - GFF578 0.48 +1.0 623,227 - GFF578 0.48 +0.0 623,227 - GFF578 0.48 +0.3 623,227 - GFF578 0.48 -0.3 623,227 - GFF578 0.48 +0.2 623,227 - GFF578 0.48 -0.3 623,227 - GFF578 0.48 +0.2 623,227 - GFF578 0.48 -0.7 623,227 - GFF578 0.48 -0.0 623,227 - GFF578 0.48 +0.5 623,227 - GFF578 0.48 -1.2 623,227 - GFF578 0.48 -0.5 623,227 - GFF578 0.48 -0.5
Or see this region's nucleotide sequence