Strain Fitness in Variovorax sp. SCN45 around GFF5723

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5722 and GFF5723 are separated by 10 nucleotidesGFF5723 and GFF5724 are separated by 85 nucleotidesGFF5724 and GFF5725 are separated by 57 nucleotides GFF5722 - Flagellar P-ring protein FlgI, at 91,123 to 92,331 GFF5722 GFF5723 - Flagellar protein FlgJ [peptidoglycan hydrolase], at 92,342 to 93,358 GFF5723 GFF5724 - Positive regulator of CheA protein activity (CheW), at 93,444 to 93,959 GFF5724 GFF5725 - Methyl-accepting chemotaxis sensor/transducer protein, at 94,017 to 95,576 GFF5725 Position (kb) 92 93 94Strain fitness (log2 ratio) -1 0 1 2at 91.640 kb on - strand, within GFF5722at 91.640 kb on - strand, within GFF5722at 92.604 kb on - strand, within GFF5723at 92.858 kb on + strand, within GFF5723at 92.858 kb on + strand, within GFF5723at 92.859 kb on - strand, within GFF5723at 92.859 kb on - strand, within GFF5723at 92.864 kb on + strand, within GFF5723at 93.131 kb on + strand, within GFF5723at 93.132 kb on - strand, within GFF5723at 93.132 kb on - strand, within GFF5723at 93.177 kb on - strand, within GFF5723at 93.185 kb on + strand, within GFF5723at 93.186 kb on - strand, within GFF5723at 93.300 kb on - strandat 93.317 kb on + strandat 93.980 kb on - strandat 94.031 kb on + strandat 94.258 kb on - strand, within GFF5725

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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91,640 - GFF5722 0.43 -0.3
91,640 - GFF5722 0.43 +1.1
92,604 - GFF5723 0.26 -0.2
92,858 + GFF5723 0.51 +1.1
92,858 + GFF5723 0.51 +0.7
92,859 - GFF5723 0.51 -0.2
92,859 - GFF5723 0.51 +1.8
92,864 + GFF5723 0.51 +0.8
93,131 + GFF5723 0.78 -0.1
93,132 - GFF5723 0.78 -0.1
93,132 - GFF5723 0.78 +0.7
93,177 - GFF5723 0.82 -0.2
93,185 + GFF5723 0.83 -0.6
93,186 - GFF5723 0.83 +0.7
93,300 - +0.0
93,317 + -0.2
93,980 - +1.5
94,031 + -0.6
94,258 - GFF5725 0.15 -0.4

Or see this region's nucleotide sequence